能受精卵 的英文怎麼說
中文拼音 [néngshòujīngluǎn]
能受精卵
英文
permanent ovum-
Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。During male gametogenesis, the spermatogonia became primary spermatozoa through mitosis, and then became fourth equivalent cytoplasm, round sperm through meiosis. after maturation, they participated in embryo development with the mature oocyte through fertilization. but in female gametogenesis, the primary oocyte formed a matured egg and 1 ~ 3 polar bodies ( pb )
雄性動物精子發生過程中精原細胞最後一次有絲分裂形成二倍體精母細胞后,經過兩次減數分裂形成了四個含等量細胞質、圓形的精子細胞,最終發育成四個具有正常生理功能的精子,與成熟卵母細胞受精后參與胚胎發育。Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵,實驗結果如下: ( 1 )顯微注射后,根據胚胎發育分期,胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光,原腸期發熒光的胚胎比例為25 ,後期發育熒光率逐漸下降,肌肉效應期后又相對穩定。Background : in - vitro maturation ( ivm ) of human oocytes would be an attractive alternative to gonadotrophin stimulation for in - vitro fertilization ( ivf ). it could avoid the complications of gonadotrophin stimulation and reduce the cost of drugs. however, the efficiency of existing ivm technique is suboptimal because embryos resulting from ivm oocytes exhibit frequent cleavage blocks and low implantation rate
研究背景:近20年來,輔助生育技術有了突飛猛進的發展,體外受精、卵漿內單精子注射技術( intracytoplasmicsperminjection , icsl )和種植前遺傳學診斷技術的成熟標志著人類生殖醫學進入了嶄新的領域,但同時也不能否認,在這一領域中還存在諸如妊娠率低、藥物副作用和多胎率高等問題。" either female komodo dragons are spermthecal, meaning that they have the ability to store sperm or fertilised eggs for long periods, in this case for two years, or they are parthogenetic, meaning that they are self - reproductive - they produce clones of themselves, " de la panouse said
其一,每隻雌性科莫多巨蜥都有受精囊,這意味著它們有能力在很長一段時間存儲精子或受精卵。由此看來, sungai可能就將精子或受精卵存儲了兩年之久。Because the likelihood wants eduction oosperm, the 3rd day goes to a hospital is, this is medicaments when abortion is the most crucial, and also be the gives an issue the most easily level in whole abortion process, want to arrange you to go to a hospital take the drug of the last day so
第三天去醫院是因為可能要排出受精卵了,這是藥物流產最要害的時候,而且也是整個流產過程中最輕易出問題的階段,所以要安排你去醫院去吃最後一天的藥。The mice showed also ageing - associated decline in oocyte quality, as indicated by increased frequency of premature chromatid separation, spindle disruption and / or chromosome misalignments in mii oocytes of aged mice. the gv oocytes from aged mice normally matured in vitro. the percentages of pronuclear - stage embryos, 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice, but pronuclei formed 1 h later in aged mice than in young mice
老齡小鼠gv期卵母細胞能正常體外成熟;老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異,但原核形成時間延遲1小時;在老齡小鼠卵母細胞,注射牛精子抽提物或sr ~ ( 2 + )處理時所誘發的ca ~ ( 2 + )振蕩以及在減數分裂成熟及早期胚胎中的pkc分佈未發生改變。We should try our best to reserve the salpinx during tubal pregnancy if the patients need to keep their reproductive capabilities
對于輸卵管積水行體外受精-胚胎移植者,如對側卵巢缺如或卵巢功能低下,不宜做預防性輸卵管切除術。If an egg is fertilized, the body automatically absorbs it and the extra tissue from the uterus.
如果卵子未能受精,它和子宮內的其他組織則自動地被身體所吸收。2. the gynogenesis of allotetraploid hybrids without the treatment to double the chromosome number, the diploid eggs produced by the females of tetraploid hybrids of red crucian carpx common carp were able to develop to living diploid fish after subjecting to activation of the uv - irradiated sperm
人工誘導異源四倍體鯽鯉的雌核發育異源四倍體鯽鯉能產生二倍體卵子,用uv處理鏡鯉的精子,不進行染色體的加倍處理,能獲得二倍體雌核發育魚,通過冷休克對「受精卵」進行處理,仍然獲得了二倍體雌核發育魚。Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control
目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響,探討內毒素影響體外受精結局的環節及可能的機制,確定三個體外培養系統對內毒素的敏感性,以期為臨床和實驗室質量控制提供參考。Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。If a woman does not oulate there is no egg for sperm to fertilise and so she cannot become pregnant
如果一位婦女不能排卵,那麼就不能形成受精卵,也就不會懷孕。It is also seen in in vitro fertilization as several fertilised eggs are implanted into the body to increase the chance of conception
多胎妊娠可能是使用促性腺激素治療不育,或在進行體外受精時,為了增加受孕機會而將多個受精卵植入體內的結果。A human egg, with its nucleus swapped for that of a patient ' s skin cell, can behave as if it has just been fertilised by a sperm and grow into a blastocyst
如果將一個人類卵細胞的細胞核換成病人的表皮細胞,它就能像剛經過精子受精一樣發育並成長為胚泡。The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共顯微注射32197粒草魚受精卵,出苗12945尾草魚魚苗,經孵化培育最後獲得1120尾五寸左右草魚,對1120尾草魚提取血液總dna進行pcr和southern雜交檢測, 2000年轉導的草魚的pcr檢測結果,有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶,說明轉化個體整合有huj ifn基因,經southern雜交進一步證實,轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合,但整合位點沒有固定區域,整合的拷貝數存在較大差異。In this study, we showed that the pdk inhibitor ly294002 regulated the cellular localization of materials 1. kunming mice were supplied from the department of laboratory animals, china medical university 2. pregnant mare serum gonadotropin ( pmsg ) and human chorionic gona - dotropin ( hcg ) were obtained from tianjin huafu biological products research institute and shanghai products research insititute, respectively
為了研究在小鼠受精卵中pkc 、 pkb是否也通過p21蛋白參與調控g2 m轉換,本實驗應用了pma和ly294002作為pkc 、 pkb的抑制劑,研究它們對小鼠受精卵中p21蛋白表達和定位,進一步探討可能的調控機制。These suggest the bsp proteins and its homologous proteins are ubiquitous in mammals and must have some physical significances. in our research, to find and study the important role of bsp proteinsand their related proteins in the development of zygotes and to find the new gene related to bsp, we cloned a cdna gene sequence related to bsp proteins and make it express in eukaratic cells. the fusion protein acquired are purified are used to deduce its biological functions
而在我們的實驗中為了發現和研究bsp及其相關蛋白在受精卵的發育中的重要作用,尋找bsp相關的新基因,克隆了一個與bsp蛋白相關基因的山一na序列並將該基因編碼的蛋白質在原核細胞中進行了表達和純化,將純化后的融合蛋白作了一些生物學功能方面的初步工作,發現新蛋白對tpk有一定的抑制作用,而tpk是細胞生長和代謝中十分重要的關鍵酶,這對于進一步研究新蛋白的生物學功能具有十分重要的意義。At water temperature of 23, the fish larvae cannot be hatched from the fertilized eggs in water with a salinity below 16 or above 48, the fish larvae can be hatched from the fertilized eggs in the salinity range of 18 to 46, the fish larvae were all abnormal in water with a salinity above 38, the salinity has no effect on the hatch time, and the suitable hatch salinity range is 28 to 32
在水溫23下,受精卵在鹽度16以下及48以上均不能孵化出仔魚,在鹽度18 ~ 46的范圍內,受精卵均可以孵化出仔魚,鹽度38以上均為畸形,且孵化時間不受鹽度的影響,其適宜孵化鹽度為28 ~ 32 。The fish larvae cannot be hatched from the fertilized eggs at temperature below 18 or above 28, the fertilized eggs can be hatched at temperatures 18 to 28, the suitable hatch temperatures are 22 to 23, and the hatch time of fertilized eggs gradually decreased with increasing temperature
受精卵在18以下及28以上均不能孵化出仔魚,在18 ~ 28水溫條件下,受精卵可以孵化,其適宜的孵化水溫為22 ~ 23 ,且隨著溫度升高受精卵孵化時間逐漸縮短。分享友人