脫毒肽 的英文怎麼說
中文拼音 [tuōdútài]
脫毒肽
英文
peptide-
The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板,加入制備好的肽庫,用tbst洗去非特異結合的噬菌體,加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ,室溫放置10min以洗脫特異結合的噬菌體, 2mol ltris - hcl ( ph9 . 1 )中和后,取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定,其餘噬菌體擴增後用于下一輪篩選,共重復3輪淘洗。This study was to investigate the effects of sulfur dioxide inhalation at different concentrations on some glutathione - related enzymes such as glutathione s - transferase ( gst ), glucose 6 - phosphate dehydrogenase ( g6pd ) and glutathione reductase ( gred ) in brain, lung, heart, liver, kidney and spleen of mice by the technology of biochemical toxicology. the results were showed as follows, so2 exposure at different concentrations caused the changes of glutathione redox system. moreover, the activities of antioxidative enzymes and the contents of reduced glutathione ( gsh ) were decreased significantly in different tissues at higher concentrations of soa
本研究利用生化毒理學技術研究了不同濃度二氧化硫吸入( 22 2mg m ~ 3 , 64 3mg m ~ 3 , 148 23mg m ~ 3 )對純系昆明小鼠腦、肺、心、肝、腎、脾六種組織的谷胱甘肽還原酶( glutathionereductase , gred ) 、谷胱甘肽硫轉移酶( glutathiones - transferase , gst )和葡萄糖- 6 -磷酸脫氫酶( glucose6 - phosphmedehydrogenase , g6pd )活性的影響,結果表明so _ 2吸入使小鼠不同組織的谷胱甘肽氧化還原系統發生了改變,表現為隨著so _ 2吸入濃度的增加,該系統中的抗氧化酶活性的顯著變化和抗氧化物質水平的顯著降低,且存在著組織差異性。
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