脫氨基酶 的英文怎麼說
中文拼音 [tuōānjī]
脫氨基酶
英文
deaminase-
Cloning and expression of l - cysteine desulfhydrase from pseudomonas sp. ts
半胱氨酸脫巰基酶基因的克隆與表達Decarboxylase an enzyme that catalyzes the decarboxylation of carboxylic acids, including the conversion of amino acids to amines
脫羧酶:能夠催化羧酸進行脫羧反應的酶,包括氨基酸轉化為胺的反應。Other : the dyewood is known as the suppression histidine decarboxylase, the catecha phenol - o - methyl shift enzyme action
其它:染料木素有抑制組氨酸脫羧酶,兒茶酚- o -甲基轉移酶作用4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk ( arod : : arobkan ~ r )為了改變代謝途徑脫氫奎尼酸( dhq )分支點上的代謝流量,使之充分流向目的產物奎尼酸合成方向,利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體,使dhq脫水酶( dhqase )失活,阻斷了碳代謝流流向芳香氨基酸生成的方向,同時用同源重組的方法將arob基因定位整合入染色體上,解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響,並減輕代謝負擔。The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
(二)寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析,在檢測的9個酶系統中,共檢測到11個酶位點:天冬氨酸轉氨酶( l個位點, 2個等位基因) ,堿性磷酸酶( 2個位點, a加了和a加2各有2個等位基因) , r澱粉酶( l個位點, 2個等位基因) ,醋酶( 2個位點, es巧和est7各有2個等位基因) ,蘋果酸脫氫酶( l個位點, 3個等位基因) ,蘋果酸酶( l個位點, 2個等位基因) ,乳酸脫氫酶( l個位點, 4個等位基因) ,磷酸葡萄糖轉氨酶( l個位點, 3個等位基因) ; a澱粉酶為單態。However, glybetaine does not accumulate in many important crop plants such as potato, tomato or rice. among plants glybetaine biosynthetic pathway has been thoroughly characterized in sugar beet and spinach, and biosynthesis is localized in the chloroplast by a two - step oxidation of choline via the intermediate betaine aldehyde. the first enzyme, choline monooxygenase ( cmo ) and the second enzyme are all localized in chloroplast
甘氨酸甜菜堿是由膽堿起始的兩部催化反應完成的,第一個酶是膽堿單氧化酶( cmo ) ,已經被純化,並且它是定位在葉綠體基質中;第二個酶是甜菜堿醛脫氫酶( badh ) ,它也主要定位於葉綠體中。The common biosynthesis pathway of aromatic amino acids includes seven steps from dahp to chorismate acid. for the common pathway, 3 - dehydroquinate ( dhq ) synthase ( encoded by arob ), 5 - enolpyruv - oylshikimate s - phosphate ( epsp ) synthase ( encoded by aroa ), and chorisma - te synthase ( encoded by aroc ] are rate - limiting enzymes
芳香族氨基酸的合成步驟有七步是共同的,亦即從dahp到分支酸的合成步驟,其中脫氫奎寧酸合成酶( arob ) 、 5 -烯醇式丙酮酰莽草酸合成酶( aroa )和分支酸合成酶( aroc )是此代謝途徑的關鍵酶。The association of gene polymorphisms of mgp and alad with lead blood level in children
羧基谷氨酸蛋白和氨基乙酰丙酸脫水酶基因多態性與兒童血鉛的關系Badh cdna ( 1901bp ) included a 66 bp 5 " utr, a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach, sugar beet and atriplex hortensis respectively. the deduced amino acid sequence included a decapeptide sequence " vtlelggksp ", which is highly conserved among general aldehyde dehydrogenases ( aldh ), and a cysteine residue
Badhcdna全長1901bp , 5端非編碼區66bp , 3端非編碼區329bp ,含有2個可能的加polya信號: aataa ,開放閱讀框架1506bp ,編碼一個由501個氨基酸構成的多肽,與菠菜、甜菜、山菠菜badh的氨基酸序列同源性均為88 ,其中有醛脫氫酶的保守序列vtlelggksp和半胱氨酸殘基。Chorismate acid is branch point in aromatic amino acids biosynthesis, related to phenylalanine, bifunctional enzymes chorismate mutase / prephenate dehydratase ( encoded by phea ) is rate - limiting enzyme
分支酸是芳香族氨基酸合成途徑的分支點,與苯丙氨酸合成有關,雙功能酶分支酸變位酶-預苯酸脫水酶( phea基因編碼)是關鍵酶。The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外,本文還選用咖啡酸純品來突破切割體系與識別體系(用氨基臂修飾的寡聚脫氧核苷酸)的連接,並用高效液相色譜法分析其偶聯產物,為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟,得到sds - paeg電泳純,通過n末端氨基酸序列分析驗證酶蛋白的純度。Inhibitor of aromatic l amino acid decarboxylase
芳香氨基酸脫羧酶抑制劑Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株,在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術,通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ,將其克隆到puc19載體中,經酶切鑒定、 pcr鑒定篩選出重組陽性質粒,並測序鑒定,通過美國blast程序進行了基因數據庫相似性比較分析。It ' s pi is 5. 2 as determined with ief. amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala, asp, leu, ser, glu, thr, phe, lys in it
氨基酸組成分析表明:枯草芽孢桿菌6 -磷酸葡萄糖酸脫氫酶的亞基由約480個氨基酸殘基組成,富含丙氨酸、門冬氨酸、亮氨酸、絲氨酸、谷氨酸、蘇氨酸、苯丙氨酸、賴氨酸。Gene expression of neural related genes was identified by semi - quanti - tive rt - pcr analysis and genechip assay. 4 and 10 days after neural induction, gene expression pattern was analysed by genechips, which showed the expression of some neural stem cells and mature neurons specific and related genes, repectively. especially the expression of gabar and glutamate dehydrogenase ( gad ), which meant the induced cells could be gabanergic neurons
2 .基因晶元檢測到未分化escs 、神經幹細胞及成熟神經細胞的相關基因,並由半定量rt一pcr證實基因晶元檢測未分化細胞表達胚胎幹細胞特異基因;誘導后第4天細胞高表達神經幹細胞特異性基因;誘導后第10天細胞高表達成熟神經細胞特異性基因,且有gaba受體、谷氨酸脫梭酶( gad )表達,說明誘導后細胞大多為以ba能神經元。分享友人