脾刺激法 的英文怎麼說
中文拼音 [bìcìjīfǎ]
脾刺激法
英文
splenocleisis-
These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria. the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit, and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128, 000
從制備性聚丙烯酰胺凝膠中回收融合蛋白,用mtt法測定重組蛋白刺激小鼠脾淋巴母細胞增殖反應活性,並以重組蛋白免疫家兔制備抗豬il - 6滴度為128 , 000的高免兔血清。To evaluate cell - mediated immune response, level of ifny secreted by splenocytes of immunized mice which upon antigen - specific stimulation was detected by using a cytopathic effect reduction assay and splenocytes proliferation of immunized mice in response to antigen restimulation was measured by mtt method
為了檢測mpt64免疫小鼠體內細胞免疫反應,免疫完成後第2 ) hj ,分離免疫小鼠的脾淋巳細胞,體外) fj抗原冉刺激,生物學方法檢測11 。Design : viable mtb were detected in mesenteric lymph nodes, spleen and lungs at day 15, 30and 60 after intragastric administration of bcg to mice : the proliferation of t - lymphocyte with the stimulus of the purified protein derivative ( ppd ) was measured at day 60 and 90 after intragastric administration of bcg to mice ; the expression of interleukin - 2 ( il - 2 ) of t - lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of bcg
方法:在小鼠胃內接種bcg的第15 , 30 , 60天,檢測結核桿菌在小鼠體內臟器的定植;接種bcg的第60 , 90天,檢測小鼠脾淋巴細胞受到ppd刺激后轉化試驗小鼠脾淋巴細胞受到ppd刺激后il - 2表達量。
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