腖酶 的英文怎麼說
中文拼音 []
腖酶
英文
peptolytic eozymes-
It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖，最佳比例為2 ： 3 ，最佳濃度為1 . 6 ；加入其它碳源時有助於菌株穩定產酶； 100ml搖瓶的最佳裝液量為15ml ；肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶，其中肌酸誘導產酶的效果最好，而肌酐和尿素不能誘導菌株產酶；誘導物肌酸的最適加入時間為接種培養12小時后，最適加入量為0 . 75 。The growth of f - 16 and the production of enzyme were affected by microbial medium, including c source, n source, mineral, initial ph of medium, rotating rate, culture time and culture temperature. the results showed that the optimal n sources were peptone, corn steep liquor and yeast extract ; the optimal c sources were sucrose, glucose and maltose ; the optimal minerals were mgso4 7h2o, khpcu and cuso4 5h2o
實驗表明，氮源中蛋白腖、玉米漿、酵母粉比較好；碳源中蔗糖、葡萄糖、麥芽糖這三種糖對產酶和生物量提高效果顯著；無機鹽中mgso _ 4 ? 7h _ 2o ， k _ 2hpo _ 4 ， cuso _ 4 ? 5h _ 2o對產酯酶的酶活及其反應后所得水解液的光學純度有較好的作用。
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