腸桿菌目 的英文怎麼說

中文拼音 [chánggǎnjūnmù]
腸桿菌目 英文
enterobacteriales
  • 腸 : 名詞1. （消化器官的一部分, 通稱腸子） intestines 2. （用腸衣塞肉、魚等製成的食品） sausage 3. （感情; 情緒; 情感） heart
  • 桿 : 桿名詞（桿子） pole; staff
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 目 : Ⅰ名詞1 （眼睛） eye 2 （大項中再分的小項） item 3 [生物學] （把同一綱的生物按彼此相似的特徵分為幾...
  • 桿菌 : [微生物學] bacillus
  1. Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )

    目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有（ 6 - 4 ）光裂合酶活性的基因，本研究從鹽生杜氏藻dunaliellasalina中克隆到（ 6 - 4 ）光裂合酶的基因，並將該基因在大腸桿菌中得以表達，這是首次在藻類中克隆到（ 6 - 4 ）光裂合酶基因，對光裂合酶的研究具有重要意義。
  2. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  5. Objective : to examine the contamination of e. sakazakii in imported raw milk powders

    摘要目的：檢測進口原料奶粉中是否污染坂崎腸桿菌。
  6. Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2

    含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。
  7. P - galactosidase of e. coli which is scarce in human blood plasma and has a variety of substrates, is one of the most frequently used enzyme labels. it is used both in heterogeneous and homogeneous enzyme immunoassay

    大腸桿菌的-半乳糖苷酶，因人血漿中缺乏此酶，並具有大量易得的底物，被用於均相及非均相免疫分析，是目前最常用的標記酶之一。
  8. Minnesota meat packer is recalling about 188, 000 pounds of beef that may be contaminated with e. coli

    一明尼蘇達肉類加工商目前正招回可能感染大腸桿菌的18萬8千磅牛肉。
  9. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  10. Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta. after sonication, debris was removed by centrifugation at 10000 x g for 15 min at 4

    挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。
  11. Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen, and express the target proteins by iptg induced in escherichia coli

    目的：構建截短的乙型肝炎表面抗原分子的原核和真核表達重組質粒，然後分別在大腸桿菌中誘導表達並純化表達蛋白及在真核細胞中表達目的基因，並檢測其抗原特性。
  12. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  13. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  14. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  15. In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase

    本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。
  16. In 2005, the number of isolates of enterobacter spp. tested was 1

    二零零五年，腸桿菌的測試樣本數目有1個。
  17. The full coding regions of bdnf genes were amplified from the genome of tylototriton taliangensis, phrynocephalus hongyuanensis, japalura splendida and cyclophiops major, respectively by pcr with the primers designed on the sequence of human bdnf gene. the pcr products were cloned into the vector pucis of esherichia coli. sequence analysis showed that the coding regions of three reptiles are the same ( 741 bp ) in length and these bdnf genes encode a peptide of 246 amino acid residues while that of tylototriton taliangensis is 744 bp in length and encodes a peptide of 247 amino acid residues

    根據已有的人bdnf結構基因的全長序列設計了一對引物，利用pcr技術分別從大涼疣螈（ tylototritontaliangensis ） 、紅原沙蜥（ phrynocephalushongyuanensis ） 、麗紋龍蜥（ japalurasplendida ）和翠青蛇（ cyclophiopsmajor ）的基因組dna中擴增到目的dna片段，並將其分別克隆到大腸桿菌載體中，然後對所獲得的陽性克隆進行測序。
  18. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  19. The present investigation is favorable for screening the new generation of anti - tumor peptides. [ objective ] ( 1 ) to clone and express fasl - ecd in e. coli

    [目的] （ 1 ）分析人黑色素瘤細胞（ a375 ）中fasl基因的表達，克隆人fasl胞外區的編碼基因，並在大腸桿菌bl21中進行表達。
  20. Schubert et al reported that hpi was found in 93 % eaggec, 27 % enteroinvasive e. coli, 5 % enteropathogenic e. coli and enterotoxigenic e. coli

    在不同類致瀉性大腸桿菌中， hpi毒力島在eaggec中高頻分佈的原因，目前也不清楚。
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