腸溶性 的英文怎麼說
中文拼音 [chángróngxìng]
腸溶性
英文
enteric solubility-
The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的內容包括:一、在前人對抗菌肽cmiv研究的基礎上,對n端和c端進行氨基酸保守變換,設計和合成了該基因,充分使用大腸桿菌偏愛的密碼子,並將該基因5端與硫氧還蛋白基因3端融合,通過ptxfus表達載體獲得較高可溶性表達(在15 sds - page膠上可見明顯的表達蛋白帶) 。In - soluble fibre helps solid wastes pass through the body more efficiently, and thereby reduces exposure of the intestines to toxic substances that can cause intestinal cancer. this kind of fibre also prevents or reduces the severity of constipation, hemorrhoids, and diverticulosis
非水溶性食物纖維能加速使糞便排出體外,直接減少廢物在腸道停留時間,減低有害致癌物質在腸臟積聚,能預防腸道癌,避免便秘、痔瘡及憩室病的出現或惡化。Guan et al ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 ofschistosomajaponicum, which is an internal image anti - idiotypic antibody of gut associated antigen ( gaa ), its antibody isotype is igm. np30 has a partial cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ). np30 molecule has been researched on many aspects
管曉虹等( 1991 )建立了一株日本血吸蟲單克隆抗獨特型抗體np30 ,已證實為腸相關抗原( gaa )的內影像抗獨特型抗體,其抗體同型為igm ,與可溶性蟲卵抗原( sea )及膜相關抗原( maa )有部分交叉反應。Guan xiaohong, et al. ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum, whice is an internal image antigen of gut associated antigen ( gaa ), its antibody isotype is igm. np30 has a partial cross - reaction with soluble egg antigen ( sba ) and membrane associated antigen ( maa )
管曉虹等建立的日本血吸蟲單克隆抗獨特型抗體( anti - id , ab _ 2 ) np30是腸相關抗原( gaa )的內影像anti - id ,與可溶性蟲卵抗原( sea )有交叉反應,可替代蟲源性抗原用於血吸蟲病免疫診斷和抗病疫苗的研究。Interestingly, feeding a high fibre diet, based predominantly on soluble fibre, has been shown in to increase subsequent lactation feed intakes possibly due to increased gut capacity ( table 3 )
一個很有意思的事實是:妊娠期採用可溶性纖維配製的高纖維日糧可以增加泌乳期的採食量,這可能與高纖維增大了腸道容量有關。Stability of mesalazine enter - coated tablets
美沙拉嗪腸溶片穩定性的研究Activity evaluation assay proved that ultrafilter graft was better than dialysis. 6. the comparison of gpif immunobiologic activity with the similar drugs. when we compared the immunobiologic activity of gpif to thymopolypeptides solution, thymopolypeptides enteric - coated tablets and tansfer factor solution, the checking results indicated that immunobiologic activity of gpif was higher than the similar drugs, which were produced and saled in chongqing
6 、 gpif與同類藥品免疫生物活性比較研究我們在對比gpif與所有重慶市制藥企業生產的以及在重慶市銷售的同類藥品胸腺膚注射液、胸腺膚腸溶片(膠囊)和轉移因子注射液免疫生物活性時發現,採用此標準制備的gp工f免疫生物活性高於同類產品免疫生物活性。Coli have been performed in this study. engineering bacteria, screened for efficient expression of pheromone 3, express the proteins in type of insoluble ( inclusion ) and soluble forms, respectively
本實驗對游仆蟲信息素在大腸桿菌中的表達進行了系統的比較與分析,篩選獲得了有效表達信息素的工程菌株,表達產物分別為包涵體與可溶性蛋白。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 , 4 -糖苷鍵連接而成的寡糖,它具有良好的水溶性、組織兼容性,在生物體內易降解,同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能,因此已成為眾多領域的研究熱點。The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e. coli culture contained protein 3a. the results of the study indicated that protein 3a existed in the form of inclusion body. the content of the expressed protein in the induced bacteria protein was 29. 2 % and 22. 1 % respectively
Sds - page和westernbolt結果證實,大腸桿菌菌體不可溶性蛋白中富含3a蛋白,且此融合蛋白的分子量符合預期設計,說明3a蛋白在表達產物中以包涵體的形式存在,所表達的蛋白含量分別占菌體蛋白的29 . 2和22 . 1 。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上,對cmiv抗菌肽的c端進行較大的改造,即將與腫瘤細胞過度表達的表皮生長因子受體( egfr )具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端,設計完整的基因,並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。The antiplatelet aggregation of micro capsule powder of enteric - characteristic plg
腸溶性微囊粉的抗血小板聚集作用These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 ( humanplasminogenkringle5 , hpk - 5 )因子的基因序列和蛋白質序列進行分析的基礎上,利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體;用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒,用不同的感受態大腸桿菌( eBecause of its incredible toxicity and stability, staphylococcal enterotoxin b ( seb ) is considered as a potential threat as a biological weapon of mass destruction
金葡菌腸毒素是一種可溶性耐高熱蛋白,是引起食物中毒頻率較高的毒素,也是生物戰劑中的主要毒素。The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明,融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上,融合蛋白主要以不溶性包涵體的形式存在於細胞中,少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ,並在大腸桿菌bl21 ( de3 )中誘導表達,但過量表達的蛋白主要是以不溶性蛋白形式存在。And the fusion protein could been purified quickly by thiobond ? resin. this was key step to produce cmiv largely by fermentation. meanwhile, the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell
以上研究的意義: 1 、初步解決了抗菌肽cmiv突變體在大腸桿菌中高效可溶性表達的問題,為進一步大量生產cmiv基因突變體打下基礎; 2 、為進一步設計具有特異導向性的抗菌抗腫瘤cmiv突變體基因和表達以及以後進行動植物轉化打下基礎。Objectives : in our previous study, to improve the expression efficiency of human basic fibroblast growth factor ( hbfgf ) expressed in escherichia coli, we lowered the g + c content in hbfgf ' s tir region and used more preferred codons by e. coli. meanwhile we found the low insoluble product with a large quantity of dimer and higher multimer in the protein solution. it greatly decrease the bioactivity of hbfgf
目的:我們在長期實踐中發現,大腸桿菌中表達的人堿性成纖維細胞生長因子( humanbasicfibroblastgrowthfactor , hbfgf )在e . coli中表達量不高,可溶性低,蛋白溶液中大量存在二聚體,三聚體以至多聚體,使得hbfgf的生物活性大大降低。All above showed that peacl preserved the polymerization activities of actin, and gfp fusion gave facilities for the study of microfilaments proper ties in vitro. monomer his - tagged peac1 - gfp could notably inhibit dnase i activities
上述結果表明,通過gfp的融合而在大腸桿菌中可溶性表達的peac1 ,不僅很好保持了肌動蛋白的聚合活性,而且gfp的熒光特性也方便了微絲體外特性的研究。Insoluble fibre comes mostly from wheat husks and vegetables. after absorbing water it expands and becomes soft, aids the movement of the bowels and acts as an intestinal cleanser
非水溶性纖維主要來自全麥谷類及蔬菜,吸收水份后會變軟發大,幫助腸臟蠕動,是腸道的清道夫。分享友人