花粉基因表達 的英文怎麼說

中文拼音 [huāfěnjīyīnbiǎodá]
花粉基因表達 英文
pollen gene expression
  • 花 : Ⅰ名詞1 （種子植物的有性繁殖器官） flower; blossom; bloom 2 （可供觀賞的植物） flower 3 （形狀像花...
  • 粉 : Ⅰ名詞1 （粉末） powder 2 （用澱粉製成的粉條或粉絲） noodles or vermicelli made from bean potato o...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 花粉 : [植物學] pollen花粉孢子 pollen spore; 花粉病 pollinosis; pollenosis; hay fever; 花粉蓋 stopples; ...
  • 表達 : deliver; express; show; voice; convey; communicate
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. These results show that coda gene cloned from e. coli can express specifically in the anther of both monocotyledon and dicotyledon plant species upon induction of 5 - fc, and the in vivo expression product of the gene can transform the 5 - fc used externally into 5 - fu, which leads to all or partial inactivity of anthers and / or pollens

    以上結果表明， coda基因在單子葉植物和雙子葉植物花藥中的特異性表達，能將外用的5 - fc在體內轉化為5 - fu ，導致花藥或花粉的部分和全部失活。
  3. To verify the integration of st901 gene into transgenic plants genome, the stable transgenic plants were analyzed by pcr and southern blotting. the aberrant phenotypes were observed in pollens and anthers of the transgenic plants. most of pollen grains in the transgenic plant were distorted, shrunken, invagination and not stained with the solution of acetocarmine

    通過對轉基因植株花粉、花藥形態的觀察和花粉活力的測定，表明st901啟動子驅動的st901基因在轉基因植株中的表達造成花粉嚴重敗育，花粉粒皺縮，扁癟、塌陷，缺乏內容物；轉反向表達載體的馬鈴薯花粉育性僅為對照的5 . 2 ，育性下降94 . 8 。
  4. Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines

    實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。
  5. Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions

    本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。
  6. Actin filaments can not be visualized in other cell types, including stem epidermal cell, root fair cell and pollen

    在轉基因植株的其它部位，例如莖表皮細胞、根毛細胞和花粉粒中，未檢測到目的基因的表達。
  7. In this study, in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo, a kind of double labeled material was constructed. the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively

    本研究以擬南芥花粉管為材料，通過轉基因技術，將分別標記ca ~ （ 2 + ）和微絲的兩種熒光蛋白cameleon與gfp - mtalin在花粉管中同時表達，實現活體花粉管中ca ~ （ 2 + ）與微絲的同時標記。
  8. Their frequencies of gus expression in pollens were 97. 755 % 0. 800 % and 96. 556 % 0. 600 % respectively. to verify these results, the self - pollinated progenies of these two putative homozygotes were examined in t2 by histochenmical gus staining of vegetative tissues and pcr analysis using specific primers

    （ 2 ）通過對轉基因t1代的花粉gus基因表達檢測，快速鑒定和篩選得到了番茄純合植株，加速了純合體篩選的進程，證明此方法可靠，而且不會造成大量寶貴材料的浪費。
  9. The results obtained in our laboratory in the past decade years showed, apoplast calmodulin in plant kingdom may regulate a lot of growth and development process of plant, such as accelerating the proliferation of angelica dahurica suspension cells and the proplast cell regeneration, startup the pollen germination of many plants " pollen and accelerating the elongation of the pollen tube, stimulating the redox of corn root " s cell, inducing the expression of light independent rbss gene, and participating in the regulation of the restraining function of al ~ ( 3 + ) to pollen tube germination

    我室多年的研究結果表明，植物質外體cam可能做為多肽第一信使調節著植物體諸多的生長發育過程：如促進白芷懸浮細胞的細胞增殖和原生質體壁再生，啟動並促進多種花粉的萌發和花粉管的伸長，刺激玉米根細胞的氧化還原反應，誘導rbcs基因光不依賴的表達，以及參與調節胞外al ~ （ 3 + ）對花粉萌發的抑制效應等。
  10. Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule

    棉花ari ； 』基因的表達分析用地高辛將棉花arf基因標記為探針，與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花冠、胚根和胚珠的總rna進行斑點雜交。
  11. Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube. there is no detectable gus activity in other floral organs, leaves and stems. these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9

    通過對gus酶活性的組織化學定位分析，表明， st901基因啟動子驅動gus基因特異地在成熟花粉和花粉管中表達， st901基因具有花粉表達特性；且288bp （ - 297至- 9 ） （ atg定為+ 1 ）的啟動子區段足以驅動gus基因在花粉中的特異表達， st901基因啟動子的花粉順式表達元件可能位於- 297至- 9之間。
  12. In order to breed maize dwarf mosaic disease ? esistance maize species, the pap gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment, 10 transgenic maize plants were achieved and the pap gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods. the pap gene in one of the transgenic maize plants had been transcription and translated, the ability of the virus resistance is undergoing

    為了解決玉米矮花葉病害的防治問題，採用花粉介導法及基因槍法將pap基因分別導入玉米的雜交種及自交系，獲得了10株轉基因玉米， pcr及southern - blot檢測結果表明pap基因已經整合到了玉米基因組中，並且其中有一株pap基因已經得到了表達，病毒抗性鑒定表明轉基因玉米能明顯推遲玉米矮花葉病害癥狀的表現。
  13. Gus expression induced by bth was systemic and had been detected in leaf, sepal, anther and mature pollen of transgenic tobacco plants, while induction effects of sa was spatial - temporally limited. 6

    Bth能系統誘導pr - 1a野生型啟動子驅動gus基因在轉基因植株的葉、花藥、萼片及成熟花粉中表達，而sa的誘導作用則具有某種時空局限性。
  14. Screening of a potato genomic library with a full - length sb401 cdna resulted in the isolation of six positive clones. one of clones, designated 901, was further characterized

    對花粉發育過程中特異表達基因的研究，科學家們希望從分子水平上闡明小孢子發育的本質。
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