花色基因 的英文怎麼說
中文拼音 [huāshǎijīyīn]
花色基因
英文
flower color gene-
While the fruits ripening process was delayed in leetrl antisense fruits. epinastic of leaves in leetrl antisense plants was only occasionally observed, which was not observed in leetr2 antisense plants ; ( 5 ) when treated by exogenous ethylene ( 25ul / l ), the abscission of antisense leetr2 petiole explants was delayed. moreover, leaf epinasty failed to be induced by ethylene treatment c, which suggested that these tissues were insensitive to ethylene
( 5 )植株衰老和葉片脫落延遲,花瓣脫落也明顯的延遲,在轉反義leetr1基因番茄的果實成熟特性被明顯改變,表現在乙烯釋放高峰延遲,果實顏色出現變異,以及和成熟相關的酶活性的改變,然而,轉反義leetr2基因的果實的成熟特性與對照相比無明顯差別。In this experiment, seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ), exogenous salicylic acid ( sa ), nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ), then we investigated the changes of endogenous h2o2 content, the activity of the antioxidant enzymes catalase ( cat, ec : 1. 11. 1. 6 ) and ascorbate peroxidase ( apx, ec : 1. 11. 1. 11 ) and mrna levels of cat3 in different stress conditions, we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis
本實驗研究了棉花黃萎病菌?大麗輪枝菌毒素( vd - toxin )與擬南芥幼苗互作反應中外源sa 、 no供體snp 、 no合酶抑制劑nna等不同處理對擬南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表達量的影響,並對no 、 h _ 2o _ 2的積累部位進行染色檢測。Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.
關于花色結構基因查爾酮異構酶chi ( chalconeisomerase )基因對花色改變的直接報道很少,因此,本論文選用了chi基因為目的基因,以純深紅色和純白色矮牽牛( petuniahybidavilm . )為材料,研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用,並在花色改變植株中首次觀察到花器官變異。In normal greenhouse condition, coda - transgenic wheat lines ( to ) had the same plant morphorlogy and pollen i2 - ki staining rate as untransformed control plant. after treating with 300 mm of 5 - fc, however, changes in configurations of spikelet, floret and anther have been observed in the transgenic lines but not in the control, and 50 % gus - positive lines displayed outside - opened glume, abnormal stamen, smaller and thinner anther, shorter filament, and failure of selfing. in parts of 5 - fc - treated transgenic lines, the pollen staining rate by i2 - ki was much lower than that of untransformed control
溫室栽培的轉基因小麥苗( t _ 0 )未噴5 - fc處理時植株外部形態和花粉碘-碘化鉀染色的著色率與未轉基因的對照沒有差異;用300mm的5 - fc處理后,發現有50 gus陽性株系與對照有明顯的區別,表現為小穗穎殼外張,花絲短縮,花藥發育不良,較小、黃白色且花粉粒少,自花不授粉,無外來花粉授粉則不結實。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding. ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator, and has been well studied in arabidopsis thaliana
為利用轉基因技術創造甘藍型黃籽油菜,本文克隆了與甘藍型油菜( brassicanapus )色素生物合成有關的ban基因同源片段,並探索了甘藍型油菜花序浸泡法轉基因新方法。Flaming tulips. blue roses. what dutch growers of old and dr tanaka ' s employers both grasped is that rarity, and hence economic value, can be created by genetic manipulation
火紅的鬱金香,藍色的玫瑰。荷蘭老一代花卉栽培者和田中博士的老闆都明白,物以稀為貴,而「稀」則可來自基因調控。In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level
本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆,通過核酸序列的差異比較分析,研究了該基因在不同物種中的進化關系;同時,通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究,獲得如下研究結果: ( 1 )通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。Foreign genes, such as reporter genes, disease - resistant genes or color - related genes, were successfully transferred to roses via agrobacteria or through microprojectile bombardment
通過農桿菌介導法和基因槍法,外源基因如報告基因、抗病基因和改變花色的基因等已轉化成功。Butterfly bettas have the mutant gene that causes variegated fins with a very specific fin pattern
蝴蝶斗魚因基因突變擁有色彩斑駁的鰭及特殊的花色。Influence of the maize lc regulatory gene on flower color of transgenic tobacco and petunia
對轉基因煙草和矮牽牛花色的影響Researches on pigmentation of flower color, types of flower pigments, biosyntheses of anthocyanins, genes of flower color, and strategies and methods of genetic engineering of flower color were described to various degrees in this paper
本文從花的成色作用和花色素種類入手,介紹了花色苷的生物合成,並從花色基因的種類和克隆、花色基因工程操作的策略和方法等角度綜述了近年來觀賞植物花色基因工程的研究進展。Upon inoculation of cucumber mosaic virus ( cmv ), the symptom development in the transgenic plants was delayed, one week later than that of the non - transgenic control, and the symptom on the transgenic plants were much milder
Pcr反應以及southern雜交實驗表明,外源基因片段已經插入到番茄的染色體中。用黃瓜花葉病毒cmv接種轉基因番茄植株,其癥狀出現的時間比非轉基因番茄對照要延遲7天左右。For its advantages over traditional breeding technologies, genetic engineering has made a rapid progress in flower color breeding of ornamental plants
基因工程技術在觀賞植物花色育種上可彌補傳統育種技術的缺陷,因此它在花色育種方面的研究和應用發展迅速。Our data demonstrated that extracellular ca2 + was required for blue - light - induced anthocyanin accumulation and chs gene expression and verapamil sensitive plasma membrane ca2 + channel played a positive effector on bl response. heparin, an antagonist of ip3 receptor increased the anthocyanin content induced by bl but inhibited chs gene expression
Ip3與cs通道受體結合抑制劑肝素處理可促進藍光誘導的花色素苦積累,但抑制chs基因表達; ip3分解抑制劑lic13則抑制花色素著積累而促進基因表達;施加肌醇既促進藍光誘導花色素著積累又促進基因的表達。Inositol supplied in the medium enhanced both blue - light - induced pigmentation and gene expression. antagonists of cam trifluoperazine ( tfp ) and w7 were used for detecting the role of cam in bl response
鈣調素kam )桔抗劑tfo和w7對花色素昔的積累和chs基因表達的作用有抑制也有促進。Chs gene expression induced by bl was enhanced by a23187 and inhibited by egta and verapamil. but the effect of another ca2 + channel blocker nif on the gene expression was opposite to its role in the inhibition of anthocyanin accumulation
A23187對藍光誘導的chs基因表達有一定的促進作用, egta和異博定則有抑制作用;尼群地平( nif )對花色素著積累和基因表達的作用不一致,需要進一步研究。There should be a fine regulating process of ca2 + / cam in the blue - light - induced anthocyanin accumulation and chs gene expression in arabidopsis
藍光誘導的花色素音積累和chs基因表達可能存在著精細的caz 」 cam信號途徑。It has been an important aspect that the color of flower was improved by means of genetic engineering in ornamental plants
利用基因工程改良花卉顏色,已經成為觀賞植物品質育種中的重要內容。The transgenic plants ( to ) and untransformed control ones in normal greenhouse condition had no notable differences in morphlogy and pollen germination rate in vitro. however, treated with 150 mm 5 - fc, more than 90 % of transgenic lines showed pollen sterile to some extent, and the lowest germination rate was 3. 4 %, about 1 / 20 of that of the control. some transgenic lines had some changes in anther and stigma size, anther color, pollen number and quality
溫室栽培的轉基因煙草苗( t _ 0 )未噴5 - fc處理時,植株外部形態和花粉發芽率與未轉基因的對照沒有可見的差異,但用5 - fc ( 150mm )處理后, 90的轉基因煙草的花粉有不同程度的敗育現象出現,最低花粉萌發率為3 . 4 ,是對照的1 25 ,同時少量植株還伴隨有花型,花藥、柱頭大小,花藥色澤,花藥內花粉的飽滿程度等的改變。分享友人