苜蓿根瘤菌 的英文怎麼說
中文拼音 [mùgēnliújūn]
苜蓿根瘤菌
英文
rhizobium melioti-
Growth and nodulation competitiveness of poly - 3 - hydroxybutyrate metabolism mutants of sinorhizobium meliloti and the effects of exogenous biotin
苜蓿根瘤菌聚羥丁酸代謝突變體的競爭生長和結瘤能力以及外源生物素的影響Compared to 042bm, the noea deletion mutant 042bma - km showed different degree of increase in nodule number, weight of nodule nodule and plant top dry weight on cultivars of putong zihua, baoding, ningxia, baifa and aohan, but decrease on milu. however, this mutant has no significant change in ability to nodulate cultivars of huanghou and zahua. hence, noea is involved in cultivar - specific nodulation
與苜蓿中華根瘤菌042bm相比，敲除noea的突變株042bma - km在普通紫花、保定、寧夏、百發和傲漢苜蓿品種上的結瘤數、根瘤鮮重和植株地上部分的干重都有不同程度的增加，而在秘魯苜蓿品種上的結瘤數和植株地上部分的干重明顯下降，在皇后和美國雜花苜蓿品種上則沒有明顯的變化。Dna fragments containing rst - 0x1 and rst - 0x2 as well as their promoters were abtained by pcr. the results of sequencing and blast analysis suggested these fragments shares 99 % identity with sinorhizobium meliloti 1021. function of rst - oxl was unknown, while rst - 0x2 shares 81 % identity with gshb gene of rhizobium tropici, which encoding glutathione synthetase
序列測定和blast分析表明，它們和苜蓿中華根瘤菌1021的相應基因有99同源性， 042bm的rst - 0x1基因功能未知，而rst - 0x2基因和熱帶根瘤菌谷胱甘肽合成酶基因gshb有81同源性。Glutathione paritcally restores the salt - tolerant ability of 042bm - x2, and we hypothesized that glutathione plays an important role in the cells of sinorhizobium meliloti 042bm by affecting its ability to regulate intracellular k + levels
從外源加入谷胱甘肽，能夠部分恢復042bm - x2耐鹽性，推測谷胱甘肽通過調節細胞內的鉀離子濃度，在苜蓿中華根瘤菌042bm滲透脅迫反應中起重要作用。And eric - pcr fingerprinting analysis indicated xj96077 was identical with other s. meliloti at similarity level of 71 %. these results showed that xj96077 belong to sinorhizobium meliloti
同時， eric - pcr指紋圖譜的分析表明，該菌與其他苜蓿中華根瘤菌的相似性達到71 % 。Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。042bm noea was obtained by pcr, it is identical to that of strain 1021 at 99 % level, and similarity of their noea is 97 %
通過pcr擴增，獲得了042bm的noea基因。該基因與苜蓿中華根瘤菌1021noea的同源性為99 ，而與noea的相似性為97 。分享友人