It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個
菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試
菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真
菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據
形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬
菌株基因組洲a快速提取的方法; 12 .傳統的
形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證
This study adopted the ion compound antibacterial to produce the materials of antibacterial glass. two kinds of different carriers are used in this experiment, phosphate and borate system. the antibacterial glass material, which is added ag +, zn2 + through some carriers, has excellent antibacterial property against escherichia coli and staphylococcus aurous
實驗中採用兩種不同的玻璃載體體系,即磷酸鹽載體和硼硅酸鹽載體,將銀、鋅離子以一定的方式直接加入到玻璃生產的配合料中,一次性熔製成
形,能夠制備出對大腸桿
菌、金黃色葡萄球
菌等細
菌具有良好抗
菌效果的抗
菌玻璃材料。
Electron microscopy showed that virulent pseudomonas solanaceanim grown in gathering form and avirulent pseudomonas solanaceanim did in free form. so that two different monomers were estimated to comprise pseudomonas solanaceanim the research above will expand application of traditional hplc and deepen its potential in bacteria studying
結合電鏡觀察,證明強致病
菌株主要是以聚集
形式生長,而弱致病
菌株則以游離分散的
形式生長,並可以推測青枯
菌可能存在兩種不同的單體。
The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation
培養48小時,桿狀細胞的表面結構發生很大的變化,此時的
菌體表面已產生大量的粘性多糖,這些粘性多糖因
菌體在纖維素表面滑動而在
菌體表面
形成突起,即在纖維素被旺盛降解部位的
菌體表面產生了大量突起;而產生突起的
菌體深入到纖維素分子內部,纖維素表面可以清晰地看到由於
菌體嵌入纖維素分子內部而留下的凹陷。
Interferon may also be induced by bacteria, some bacterial endotoxins.
某些細
菌、細
菌內毒素都可能誘導干擾素的
形成。
The properties of morphology and physiology of this strain was studied. according to bergey ' s manual of determinative bacteriology, the strain was identified as a new strain of the sporocytophaga
通過對其
形態及其生理生化特性的研究,該
菌株被鑒定為生孢噬纖維細
菌屬( sporocytophaga )的一個新
菌株。
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬
菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來
形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿
菌,制備細
菌形式的mg _ 7重組噬
菌體抗體庫;通過
菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬
菌體抗體庫的容量和重組率。
Mushroom outlet closing and opening by hand to be inspected
菌形風帽手動啟閉試驗。
Over - expression of yggg retarded the cell cycle, on the point after dna partition, resulting in accumulation of diploid as bacteria stop division and sequentially went to death. this appearance is similar to that of the era mutants, including the partially defective in era gtpase activity or the reduced in the synthesis of wild - type era which bacteria become arrested in the cycle at the predivisional two - cell stage
在熒光顯微鏡下細
菌形態的變化,以及dapi染色和透射電子顯微鏡觀察, yggg的表達與era突變(使era功能降低時)對細
菌分裂的影響有類似之處,即細
菌dna合成,子代dna分離后;分裂停滯,
形成細
菌繁殖受阻時, 2 - 4倍體的出現。
Specification for mushroom head aluminium alloy rivets
菌形頭鋁合金鉚釘規范
Mushroom ventilators for ship
船用
菌形通風筒
Study on calibration and discrimination of pathogenic appearance of bacterial vaginosis with sialidase method
唾液酸酶法校正和區分細
菌性陰道病病原
菌形態研究
Damp conditions are needed for the formation of mould
潮濕的環境是?菌形成所需的。