菌簇 的英文怎麼說

中文拼音 [jūncù]
菌簇 英文
bacterioflora
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 簇 : Ⅰ動詞[書面語] （聚集; 簇擁） crowd together; form a cluster; pile up Ⅱ名詞（聚集成的團或堆） clus...
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. Using pij903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. okb and 1. 5kb in size respectively, were inserted into it

    Scp2 *的應用有兩個主要的限制，一是必須有被克隆基因簇的完整而詳細的遺傳學信息，二是要求它在被克隆基因簇的來源菌株中不能復制。
  3. Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins

    將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。
  4. In addition, the evident crossing - infection happened in the strains came from myrica, casuarina also could be found in the sequencial analysis. all these results we obtained from the sequencing and rflp analysis were partly accorded with what baker brought forward in 1987 ( the four host specific group, hsg ). however, they also indicated the limit of this hsg

    5株供試菌株與其它已發表菌株的全序列比較結果可將所有菌株大致劃分為4個簇，楊梅菌株fmr61 、木麻黃菌株fcg07和木麻黃菌株fce42具有較高相似性與榿木菌株聚為第簇；而楊梅菌株fmr16和2215與來自胡頹子科和沙棘的菌株聚在一起歸為第簇。
  5. Analysis of the sequence revealed that adda resembled nifs of nitrogen - fixing bacteria and dnda. the entire add gene cluster showed 78 % identity to dnd gene cluster from s. lividans

    同源性比較揭示add基因簇的adda基因與固氮酶基因nifs高度同源， add與變鉛青鏈黴菌dnd核苷酸的同一性為78 。
  6. In order to facilitate the study of biological function of add gene cluster, e. coli - s. avermitilis intragenus conjugation system was established. in addition, phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed

    建立了除蟲鏈黴菌的接合轉移系統，並構建了用於置換全部基因簇的基因置換質粒phz2114和adda的基因中斷質粒phz2130 ，為研究除蟲鏈黴菌add基因簇的生物學功能奠定了基礎。
  7. The resulting derivative strains produced antifungal activity in a manner different from the parental strain, indicating the original promoter was replaced with the engineered promoters and the epitopes

    衍生菌株合成抗真菌抗生素的產量與親本菌株不同，表明fr - 008基因的原始啟動子已被改造后的啟動子和抗原決定簇所代替。
  8. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  9. The core part of hpi comprises genes for the biosynthetic, regulatory and transport of ybt. recently, the hpi appears to be widespread in various enterobactia strains, for example, in disrrheagenic e. coli strains, citrobacter diversus, and various species of klebsiella and non - i serotypes of salmonella enterica. now, the research on yersinia hpi in the enterobactia is reported mostly from the vie wpoint of molecular epidemiology, but the structure and function of hpi in these strains is unclear

    耶爾森菌的hpi有一個約30 . 5kb的功能核心區，它攜帶有與ybt合成、調節和攝取有關的基因簇irpl irp9等基因，其中， irpl irp5及irp9基因與ybt合成有關， irp6 、 irp7 、 fyua基因與其轉運有關， irp8基因功能不明， ybta基因對ybt的合成攝取起調節作用，天門冬氨酸trna （ asn - trna ）是hpi的整合位點。
  10. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
  11. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces

    為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。
  12. And the positive clones were used as the immunogens to immunize mice. both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell )

    檢驗這種短肽可否模擬vegf的抗原決定簇誘發機體產生能與vegf結合的抗體，研究小鼠抗血清對huvec （人臍靜脈血管內皮細胞）生長的抑制作用以及噬菌體表達的7肽和12肽對huvec生長的抑制作用。
  13. The dnd cluster seemed to express when it was carried by the high copy - number plasmid because complementation of individual dnda, dndb or dndc mutation could be observed and such expression did not seem to influence the normal dnd expression in wild type s. lividans 1326

    而在高拷貝質粒上的dnd基因似乎是李愛英變鉛青鏈黴菌dna異常修飾系統的分子生物學研究能表達的，因為它能紅補分別在dn劇、 dndb和咖jc發生單個基因突變的菌株，而且這種表達似乎也不會影響野生型菌株1326中原有的dnd基因簇的表達。
  14. In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226

    將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。
  15. Virulence genes of pathogenic bacteria can be located on the chromosome where they may be organized as so - called pathogenicity island ( pai )

    細菌毒力島（ pathogenicityisland ， pai ）是指染色體上成簇排列的與細菌毒力密切相關的基因簇。
  16. In order to obtain a dna fragment containing aved gene, 5 ' end primer and 3 ' end primer, consisting of 18 and 20 nucleotides respectively, were designed and synthesized. a 2. 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template

    為了得到aved基因片段，首先根據已知的阿維菌素生物合成基因簇的核苷酸序列，設計併合成了由18和20個核苷酸組成的5端和3端引物；以streptomycesavermitiliss - 2基因組dna為模板，經pcr擴增得到了2 . 2kb的含有aved基因的dna片段。
  17. In immuno - blotting, these fragments reacted specifically with hepatitis c patients " sera, suggesting that e. coli - derived e2 proteins carried hcv e2 - specific, glycosylation - and - conformation - independent epitopes

    在免疫印跡檢測中，上述片段與丙型肝炎病人血清有特異性反應，表明大腸桿菌系統表達的e2蛋白攜帶有hcve2特異的、不依賴于糖化和立體構象的抗原決定簇。
  18. By immunizing rabbits with aa 192 - 326 fragment, polyclonal sera were obtained that were able to recognize e1 proteins expressed in both e. coli and mammalian cells, suggesting that e. coli - derived e2 proteins carried hcv e1 - specific, glycosylation - and - conformation - independent epitopes

    利用aa192 - 326免疫家兔，獲得了可識別大腸桿菌和哺乳動物細胞表達之e1蛋白的多抗血清，表明大腸桿菌系統表達的e1蛋白攜帶有hcve1特異的、不依賴于糖化和立體構象的抗原決定簇。
  19. The dnd phenotype was also found in dna of mycobacterium smegmatis mc2155 by pfge. query of the amino acid sequence of dnd cluster from s. lividance to the uncompleted genome sequence of me2155, a homologous gene with a 38 % identity to dnda was found and was tentatively named mddl

    將變鉛青鏈黴菌的dnd基因簇與mc ~ 2155的基因組序列（未測完）進行同源比較后，發現mc ~ 2155中存在dnda的同源基因（暫命名為mddl ） ，且二者在氨基酸水平上的同一性為38 。
  20. Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2. 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ). these constructs were transferred into streptomyces sp

    構建了帶有噬菌體啟動子和特異性抗原決定簇（表位）及2 . 7kbpks基因的整合型質粒，用於標記天然的fr - 008pks 。
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