菌質體 的英文怎麼說
中文拼音 [jūnzhítǐ]
菌質體
英文
mycoplasma-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way
由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化，以原生質體作受體是蘚類植物轉化的常用途徑。Bacterial chromatin body
細菌染色質體The optimum mycelial age for preparation of protoplast was at 24h time - point after inoculation and culture of conidia
制備該菌原生質體的最佳菌齡為從孢子接種起培養24h 。Protoplasted monokapyon ( pm ) were prepared from lentinus edodes strains jingxuan and wuxiang, respectively, intercross process were put in practice
摘要以香菇菌株精選和武香作為親本制備單核原生質體，將兩個親本的單核原生質體進行一一雜交。Breeding of high - yield l - isoleucine producing strain by protoplast fusion
異亮氨酸高產菌的原生質體融合育種Here we studied the relationship of various factors and the quality of protoplasts. which maybe could be the basic of moss gene targeting. results showed : inoculated the spores onto diferrent kinds of media, such as ms, benecke and knop, we found that there was no difference when the spores germinated and differentiated into cauliform soon
通過對立碗蘚的無菌培養和原生質體操作發現： （ 1 ）立碗蘚孢朔接種在無菌ms 、 benecke 、 knop培養基上，均可萌發產生原絲體，但不久便分化為莖葉體，很難長期保持其原絲體狀態，不同培養基條件下原絲體狀態有所不同。Our experiment indicates that hb - liposome - pdt has good effect to pws ' s animal model - leghorn cock comb at 0. 25 and 0. 5mg / kg drug and in combination with 20 min irradiation of 100mw / cm2
竹紅菌乙素一脂質體對來亨雞雞冠的光動力療效觀察表明，銅蒸氣激光混合光的照射下，在100mwcmz的功率密度照射20min ，給藥劑量為0Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。Study on hedgehog fungus breeding by protoplast uv - mutated
原生質體紫外誘變選育猴頭菌新菌種的研究The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Formation and regeneration of protoplasts of chaetomium globosum
球毛殼菌原生質體形成與再生研究The biological characteristics of mycelia from phellinus igniarius and culture media were studied. two kinds of culture media were suitable for the growth of mycelia. the result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia, and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation
為了最大限度地保存菌種的活力，以提高菌絲體的質量及菌絲體內活性成分的累積，本文通過對比研究，進一步對其生長基質進行篩選，明確了兩種適于桑黃菌絲生長的固體培養基：以馬鈴薯為氮源、蔗糖為碳源的培養基較適用於菌絲收集，以蛋白腖為氮源、可溶性澱粉為碳源的培養基較適用於菌種的保藏。Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression
本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射於家兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。Distribution of bacterial plasmids in taiyuan segment of fenhe river
汾河太原段水體細菌質粒的分佈研究First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。But in s. mycarofaciens, there is no distinctive propionate kinase, so that the utilization rate of propionic acid is lower than that of acetic acid. for this reason, it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s. mycarofaciens and s. erythreus, which would transfer propionate kinase of s. erythreus to s. mycarofaciens. therefore, the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ), which would reduce the production costs
根據文獻報道，紅黴素鏈黴菌中的丙酸激酶對丙酸的利用率是對乙酸的利用率的13倍；而在生米卡鏈黴菌中，無特異的丙酸激酶，菌體對丙酸的利用低於對乙酸的利用，因此希望利用原生質體種間融合的方法將紅黴素鏈黴菌的丙酸激酶基因轉移到生米卡鏈黴菌中，從而使融合子能夠利用丙酸鹽作為合成mdma _ 1的前體，提高mdma _ 1的產量，降低生產成本。464 and erythromycin producer s. erythreus as original strains, studies were carried out for researching higher producers and enhancing content of midecamycin ( mdm ) ai, which could use propionate as precursor to reduce production costs. according to the literature, the utilization rate of propionic acid is thirteen times to that of acetic acid by propionate kinase in s. erythreus
本論文主要研究了生米卡鏈黴菌與紅黴素鏈黴菌原生質體種間融合育種，以提高麥迪黴素a _ 1 （ mdma _ 1 ）的組分，增加其對丙酸鹽的利用度，降低生產成本。A substance extracted from mycelia with benzinum was studied for the first time. five compositions were found : butylated hydroxytoluene, n - hexadcanoic acid, 9, 12 - octadecadienoic acid methyl ester, eicosane and hexacosane. the paper provided scientific basis data for researching mainly biologic characteristics and active compositions of mycelia from phellinus igniarius cultured with solid and liquid culture media
首次對菌絲體的石油醚提取物進行定性分析，確定該菌絲體中含有二丁基夯基甲苯、棕桐酸、 9 ， 12一十八碳二烯酸甲醋，二十碳烷及二十六碳烷，這五種物質均為在桑黃菌絲體中首次發現。分享友人