菌體抗體 的英文怎麼說
中文拼音 [jūntǐkàngtǐ]
菌體抗體
英文
bsomatic antibody-
Animal health analysis methods - detection of antibodies against caprine arthritis encephalitis virus by the agar gel immunodiffusion test
動物健康分析方法.用細菌培養基凍膠免疫擴散試驗檢測抗山羊關節炎腦炎病毒抗體However, the receptor and translocation domains of the colicin ia can only recognize the inner and outer membrane receptors of the escherichia coli, therefore the wild type colicin ia can not be developed into antibiotics against other types of bacteria
然而由於其信號識別結構域只能識別大腸桿菌外膜和內膜上的特異受體，因此野生型大腸菌素難以拓展成為對抗它種細菌的抗菌素。Demonstrable antibodies are evoked in carriers after a few weeks of infection.
在感染幾星期之後，從帶菌的物體中能證實存在抗體。The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds. the last round enriched phage clones were used to reinfect
由鼠源噬菌體抗體庫淘選到展示有人絨毛膜促性腺激素單鏈抗體的噬菌體克隆。Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。The body fluid purified protein derivative antibody tests : diagnosis value in pediatric tuberculosis
結核菌素抗體對兒童結核病的診斷價值The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。Animal health analysis methods - control protocol for reagents for detection of antibodies directed against brucellosis bovine by the elisa method in bulk tank samplessamples
動物健康分析方法.用elisa方法在集液罐試樣中檢測抗布魯氏菌的抗體用試劑的控制協議Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library
從噬菌體抗體庫中淘選人絨毛膜促性腺激素的單鏈抗體Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應用重組噬菌體抗體庫技術，構建了一個針對牛精子的噬菌體抗體文庫。Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。Animal health analysis methods - detection of antibodies against brucellosis by the rose - bengal plate test
動物健康分析方法.用玫瑰紅板試驗檢測布魯菌病抗體Animal health analysis methods - detection of antibodies against brucellosis by the serum agglutination test
動物健康分析方法.用血漿凝結試驗探測布魯氏菌病抗體Animal health analysis methods - detection of antibodies against bovine brucellosis by the milk ring - test
動物健康分析方法.用牛奶環式試驗檢測牛的布魯菌病抗體Animal health analysis methods - detection of antibodies against brucellosis by the complement fixation test
動物健康分析方法.用補體結合試驗檢測布魯菌病抗體Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out
的小鼠噬菌體抗體庫，並從中淘選出對n -肽有結合活性的單鏈抗體。分享友人