Previous researchers have always determined the sp atial distribution patterns ( sdp ) of castanopsis kawakamii with a sample - dis tance method. however, the distribution patterns may be affected by the quadrat si ze and, in the course of analysis, the density differences among the cluster plots are not considered ; therefore, differences of cluster plot size and the dispersi on degree among individuals of cluster plots can not be known. authers of this pa per have determined the spatial distribution patterns of castanopsis kawakamii population in different habitats by means of non - quadrat distance method and a nalysed the pattern intensity and grain of the sdp. the pattern intensity is defi ned with the relative density differences and the pattern grain can embody the d ispersion degree of the individuals in the plots, and the dispersion degree among the plots. the determined results are as follows. the intensities of the species range in order from strong to week : litsea mollifolia p. kawakamii i. purpure a r. cochinchinensis c. kawakamii c. carlessii d. oldphamii s. superba. the gra ins of the species queue in order from coarse to close : s. superba = litsea mollif olia r. cohinchinensis c. kawakamii = i. purpurea c. carlessii p. racemosam d. oldp hamii. these determined results tally basiclly with the results authers of this paper have got in determining the same plots by means of aggregate index access ing method. in view of this, it is held that the sdp of c. kawakamii is closely related to the habitats and biological features
前人都是採用樣方方法對格氏栲種群數量的空間格局進行測定,而格局分佈有可能受樣方大小的影響,且分析過程中沒有涉及聚塊間密度差的問題,因而無法掌握種群的聚塊大小差別及聚塊內個體間的離散程度.本研究採用無樣方距離法,測定不同生境的格氏栲種群空間格局,分析格氏栲種群格局的強度和
紋理.強度以聚塊和間隙的密度差來定義,
紋理則是體現聚塊內個體間的離散程度與諸聚塊間的分離程度.測定結果表明,格氏栲種群格局強度從高到低排列次序為:木姜子蚊母樹冬青茜草樹格氏栲米櫧
虎皮楠木荷;格局
紋理從粗到細的順序是:木荷=木姜子茜草樹格氏栲=冬青米櫧蚊母樹
虎皮楠.這一測定結果與作者採用聚集度指標測定相同樣地格氏栲種群空間格局的結果基本相符.因此,格氏栲空間格局類型及分佈與格氏栲生物學特性及生境的關系密切
Among them, selenocosmia huwena and selenocosmia hainana belong to theraphosidae while macrothele raveni belongs to hexathelidae. all of them could kill insects and some small vertebrate with their venoms. in this article, using whole - cell patch - clamp technology, we observed the effects of the venoms from the three kinds of spider on the voltage - activated tetrodotoxin - sensitive ( ttx - s ) sodium current and delay - rectified potassium current of undifferentiated ng108 - 15 cells
虎紋捕鳥蛛( selenocosmiahuwena ) 、海南捕鳥蛛( selenocosmiahainana )和雷氏大疣蛛( macrotheleraveni )均是在我國近年來發現的蜘蛛新種,前兩者屬于捕鳥蛛科,而後者屬于異仿蛛科。
Huwentoxin - i ( hwtx - i ) is a polypeptide neurotoxin isolated from the crude venom of chinese bird spider selenocosmia huwena. its molecular weight is 3750 dalton. the toxin contains 33 amino acid residues and three disulfide bonds
虎紋捕鳥蛛毒素- ( huwentoxin - )是從
虎紋捕鳥蛛selenocosiahuwena粗毒中分離得到的一種多肽類神經毒素,分子量為3750 ,由33個氨基酸殘基組成,含三對二硫鍵。
Huwentoxin xi ( hwtx ~ xi ), a serine protease ! inhibitor, consists of 55 amino acid residues with three disulfide bridges. the toxin was isolated from the venom of the chinese spider ornithochoctonus huwena by ion - exchange chromatogram - phy and reverse phase high performance liquid chromatography
本文報道從
虎紋捕鳥蛛( ornithochoctonushuwena )粗毒中,應用陽離子交換和反相高效液相色譜的方法分離到一種胰蛋白酶抑制劑,命名為huwentoxin - ( hwtx - ) 。
A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc. hwtx - v has 35 amino acid residues, with the molecular mass 4111. 4da. the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh, which consists of 6 cys, formed three pairs of disulfide bridges
本文報道從
虎紋捕鳥蛛( selenocoimahuwena )粗毒中,結合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素,命名為
虎紋捕鳥蛛毒素- ( huwentoxin - , hwtx - ) ;其天然突變體( themutantofhuwentoxin , mhwtx - )也同時純化出來。
The chinese bird spider ornithoctonus huwena is distributed in the hilly areas of yunan and guangxi in the south of china. so far several neurotoxins have been isolated from the venom of spider ornithoctonus huwena
虎紋捕鳥蛛( ornithoctonushuwena )是生活在我國南部雲南、廣西的山區,本實驗室在其粗毒中發現了許多神經肽類毒素,但對其中的非肽類組分研究甚少。
3. the expression of sox genes in different tissue of rana rugulosa wiegmann was analysed by using rt - pcr technique
3 、採用rt - pcr技術研究了
虎紋蛙不同組織中sox基因的表達。
Part two : studies l. the sox gene of rana rugulosa wiegmann was amplified by using the technique of pcr with a pair of primers which specially amplify the conserved motif ( hmg - box ) of human sry gene
二、研究報告1 、採用pcr技術,以特異擴增人sry基因hmg - box保守區的一對特異引物擴增了
虎紋蛙sox基因,優化了擴增條件。
2. using a pair of degenerate primers based on the conserved region, hmg - box, of human sry gene, eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced
2 、參照人sox基因設計了一對兼并引物,擴增了
虎紋蛙的sox基因,並對擴增產物進行了克隆和測序。
The chinese bird spider selenocomia huwena is one of the most venomous spiders in china, which distributes in southwestern hilly area of china and habitually lives in the holes underground
1 :
虎紋捕鳥蛛( selenocosmiahuwena )是在我國西南地區發現的一蜘蛛新種,屬于捕鳥蛛科( theraphosidae ) 。
The venom of the bird hunting spider selenocosmia huwena, with bodysize of 6 - 9cm, which distributes in southwestern hilly area of china and habitually lives in the holes underground, contains a mixture of compounds with different types of biological activity
虎紋捕鳥蛛( selenocosmiahuwena )是我國西南部山區發現的蜘蛛新種,屬于捕鳥蛛科( theraphosidae ) 。該蛛穴居地下,個體大,毒性強。
The contraction of isolated guinea pig ileum induced by electrical stimulus can be inhibited by hwtx - i, a toxin purified from the chinese bird spider sclcnocosmia huwenn
虎紋捕鳥蛛毒素hwtx - ( 5 10 ~ ( - 6 ) g ml )對電刺激豚鼠回腸引起的一過性收縮有非常明顯的抑制作用。
A novel low molecular compound which was effective to heart is isolated from the venom of the spider through two times reverse phase high performance liquid chromatography. its exact molecular weight is 147da determined by esi mass spectrum
採用兩次反相高效液相色譜從
虎紋捕鳥蛛中分離出一種相對分子質量為147da的成分,命名為hwtxs - 。
This 40 - residue toxin with molecular weight of 4440. 18da shows little sequence identity with other spider toxins. another neurotoxic peptide, named huwentoxin - vh, has been purified from the venom of the same spider
此外從
虎紋捕鳥蛛( selenocosmiahuwena )毒液中純化的另一種新型膚類神經毒素,
虎紋捕鳥蛛毒素一vii ( huwentoxi仆vii , hwtx一vll )的化學性質與基本生物學功能。
Huwentoxin - i ( hwtx - i ) is a polypeptide neurotoxin isolated from the crude venom of chinese bird spider selenocosmia huwena, a chinese bird - hunting spider defined as a new species of the family selenocosmia. its molecular weight is 3750 dalton
虎紋捕鳥蛛毒素- ( huwentoxin - ,簡成hwtx - )是從我國捕鳥蛛新種
虎紋捕鳥蛛( selenocosmiahuwena )粗毒中分離純化的一種多肽類神經毒素。
Tiger and tiger skin
老
虎及老
虎紋
Abstract l : the chinese bird spider, ornithochoctonus huwena, distributed in the hilly areas of yunnan and guangxi in the south of china, was recently identified as a new species and is one of the most venomous spiders in china. in our previous work, we have demonstrated that 0. huwena venom contains a mixture of compounds with different types of biological activities
它的粗毒中含有多種活性成分,其中研究的比較深入的多為神經毒素,如hwtx - ,這是一種突觸前膜n型鈣離子通道阻斷劑;毒素hwtx - ,它是一種ttx敏感型鈉離子通道阻斷劑;另外,還從虎紋粗毒中純化出一種具有凝集活性毒素成分shl - 。