蛋白印跡分析 的英文怎麼說
中文拼音 [dànbáiyìnjīfēnxī]
蛋白印跡分析
英文
western blot analysis- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 印 : Ⅰ名詞1 (圖章) seal; stamp; chop 2 (痕跡) print; mark 3 (姓氏) a surname Ⅱ動詞1 (留下痕跡)...
- 跡 : 名詞1 (留下的印子; 痕跡) mark; trace 2 (前人遺留的建築或器物等) remains; ruins; vestige 3 (...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 析 : Ⅰ動詞1. (分開; 散開) divide; separate 2. (分析) analyse; dissect; resolve Ⅱ名詞(姓氏) a surname
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后, ps活性恢復是一個復雜而有序的過程,需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理,加入林可黴素阻斷葉綠體蛋白質合成,利用尿素sds變性電泳分離類囊體膜蛋白,藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式,並進行定量分析。The protein - storing cells in swietenia macrophylla were found to be populus - type, i. e. ordinary parenchyma cells containing both vacuolar protein inclusions and starch grains
用21kda蛋白質的抗血清進行兔疫印跡分析表明, 18kda蛋白質和21kda蛋白質有相同的抗原決定簇。Analysises of serum amyloid protein in senile dementia patients using immuno - slot blot
老年期癡呆患者血清澱粉樣蛋白免疫印跡分析To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression
蛋白免疫印跡分析的結果表iv明,當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時,細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平,與未經抗體處理時相比發生了變化(或升高或下降) ;而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少,使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page電泳分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的條帶。With ni2 + - lda - sepharose, the cbd tag was removed. according to the results of sds - page and western blot analysis, the product of outflow was the target protein lt a 27. the purity of lt a 27 was about 95 %
運用niz +金屬鰲合親和層析柱進一步快速純化去除融合頭,上樣流出經sds一隊ge分析和western印跡分析ii1 :明得到的純化產物為淋巴毒素缺失體蛋白lt凸27 , lt 2 :產物純度可達95 %以上。3 ) western blotting of gas1p when mutant gpi17p overexpressed showed that there was accumulation of immature gas1p. this means that there is a defect in gpi - anchoing
3 )蛋白質印跡分析表明,在突變gpi17p存在下,有未成熟gas1p的積累,表明存在蛋白質階i化缺陷。8kb cdna with 1362bp orf and codes 454 amino acids with only a sh2 domain. the gene was named as sh2a at chromosome 8p22. we study its structure by blast homologous analysis and its expression by rt - pcr and northern blot
本文將通過蛋白質分析軟體研究其結構和同源性,利用rt - pcr和northern印跡雜交技術研究該基因的表達情況及該基因與腫瘤的關系。Amino acid molecules imprinted polymeric composite microspheres with magnetic susceptibility ( ms - smips ) were prepared by suspension polymerization ( sp ) and inverse emulsion - suspension polymerization ( iesp ), respectively. protein molecules imprinted soft - wet gel composite microspheres with magnetic susceptibility ( ms - pigms ) were prepared by inverse suspension polymerization ( isp ) and seeded inverse suspension polymerization ( sisp ), respectively. the resulting ms - smips and ms - pigms were characterized by scanning electron microscope ( sem ), thermogravimetric analyzer ( tg ), vibrating sample magnetometer ( vsm ), infrared spectrum ( ftir ), high performance liquid chromatography ( hplc ), and so on
分別採用懸浮聚合法和反相乳液?懸浮聚合法制備了氨基酸分子印跡聚合物磁性復合微球( ms - smips ) ;分別採用反相懸浮聚合法和種子反相懸浮聚合法制備了蛋白質分子印跡磁性軟濕凝膠復合微球( ms - pigms ) ;用掃描電子顯微鏡、熱重分析儀、振動樣品磁強計、紅外光譜、高效液相色譜等對所制備的ms - smips和ms - pigms進行了表徵。Our recent work also suggest that simulated microgravity may induce the contractile proteins in the arterial vessles to be changed differentially. therefore, in the second part of the present work the alterations of a - sm - actin and calponin were observed by immunohistochemistry and western blotting. the major findings of the present work are as follows : 1
此外,我們的前期工作還提示,不同部位動脈血管平滑肌的收縮蛋白在模擬失重下也可能出現分化性變化,故本工作也用免疫組化及westem印跡分析方法對模擬失重大鼠血管的-平滑肌肌動蛋白( - sm - actin )及調寧蛋白( calponin )表達變化進行了觀察。The activity of the antiserum was tested by dot enzyme immunization assay ( diba ) with purified antigen. western blot of hela nuclear protein extract, which contained natural hbaf53, showed that the antiserum is also a specific antiserum to natural hbaf53. therefore the highly specific and sensitive antiserum can be applied to various studies
用純化的抗原蛋白,經斑點印跡試驗測得baf53抗血清的效價,又用提取的hela細胞核蛋白(含有天然baf53蛋白)進行免疫印跡分析( westernblotting ) ,證明天然baf53蛋白也是該抗血清的抗原,說明獲得的多抗血清具有高特異性和敏感性,可用於多方面的研究。分享友人