蛋白沉澱法 的英文怎麼說

中文拼音 [dànbáichéndiànfǎ]
蛋白沉澱法 英文
precipitation of protein
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 沉 : Ⅰ動詞1 （沉沒; 墜落） sink 2 （沉下 多指抽象事物） keep down; lower 3 [方言] （停止） rest Ⅱ形容...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 沉澱 : 1 （沉澱過程中析出的物質） sediment; precipitate; sedimentary accretion; precipitation; （doposit...
  1. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  2. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  3. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  4. According to the result showed at 280nm and at 490nm, in the comparison of whether protein absorption top and sugar quantity top overlapped, glycoproteins would be detected preparatorily, and as a result, tubes in two distinct areas had glycoproteins by this method. proteins were precipitated with trichloroaceticacid and with cold acetone, and glycoprotein was determined from sds - gel

    再從各收集管的收集液中，用三氯乙酸沉澱蛋白法、冷丙酮沉澱蛋白法相結合濃縮、制備蛋白樣品，進行sds ? page ，對sds膠進行pas糖鏈染色鑒定糖蛋白，並從茶樹葉分級蛋白中準確地鑒定出兩個區域的收集管中含有多種糖蛋白。
  5. A sds - iso - propanol method suitable for tea plant, which was plentiful of tea polyphenols, had been developed using a modification of chen darning ' s method from different sample storage conditions such as fresh, dry and frozen shoots. it was a quick, easy, economical and effective method. the tactics were as follows : before the cell nuclear membranes were decomposed, the tea polyphenols and proteins etc. were removed

    該法提取緩沖液使細胞維持一定的滲透壓，研磨時使細胞核基本保持完整；在細胞核被裂解之前去除細胞質中的茶多酚、大部分蛋白質和rna ；而後用sds裂解細胞核，異丙醇或乙醇沉澱dna ，這樣能經濟、快速和有效地從富含茶多酚、茶多糖等次生物質的茶樹新梢中提取基因組dna 。
  6. Studies to date hae shown the alue of dipsticks, precipitation, and immunoassays to detect microalbuminuria

    迄今為止，研究已經顯示了試紙法、免疫沉澱和免疫檢測法對檢測微量白蛋白尿的價值。
  7. One small captive population of amur tigers ( panthera tigris altica ), which had 14 - year captive history since its foundation in 1986, was selected for such population genetic researches in this paper, which contained three experimental parts as : ( 1 ) dna extraction from shed hairs. three methods, which were chelex, ionic detergent and non - ionic detergent, were respectively used to digest the shed hairs of one amur tiger

    本文利用chelex 、離子型和無離子型分解液三種方法對在東北虎（ pantheratigrtsaltica ）換毛期採集的自然脫落毛發進行了dna提取，並對部分毛發分解原液進行蛋白質沉澱，對于毛發分解殘留物也進行了核細胞分解與蛋白質沉澱再處理，對上述提取產物進行rapd擴增。
  8. The crude product was treated with cetyl trimethyl ammonium bromide ( ctab ) in order to separate the neutral glycoprotein and the acidic glycoprotein. the optimal condition according to elisa result was : phv. o, 1 % of crude product and 3 % of ctab

    本文採用季銨鹽沉澱法對粗產品進行部分純化，分離其中的中性糖蛋白和酸性糖蛋白，最佳分離方案為： ph = 7 . 0 ，樣品濃度為1 ， ctab濃度為3 。
  9. ( 3 ) the piezoelectric quartz crystal impedance ( qci ) technique was employed to monitor in situ the pb2 + - induced precipitation of bsa onto a gold electrode and the precipitate dissolution via edta in an aqueous solution

    （ 3 ）用壓電石英晶體阻抗法（ pqci ）現場監測了水溶液中鉛離子誘導牛血清白蛋白（ bsa ）沉澱及沉澱由na _ 2edta溶解的過程。
  10. The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively

    方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。
  11. The effective antimicrobial components were confirmed to be some non - peptide ingredients, whose molecule weight ranged from 500 ~ 3000da, by protein deposition, polysaccharide separation, ultrafiltration, nanofiltration, and ion - exchange successively

    採用沉澱蛋白、分離多糖、超濾、納濾、離子交換等方法分離水提液組分，證明芫荽中主要抑菌成分是相對分子質量在500 ~ 3000的水溶性非肽類物質。
  12. When there is a large amount of albumin in the urine, this is of little practical concern because it will be detected by fairly nonspecific methods, such as precipitation or dye binding, as well as the highly specific immunoassays

    當尿白蛋白量非常大時，這一實際操作上的憂慮變得不足掛齒，因為此時無論是使用非常不具特異性的檢測方法，如免疫沉澱或染色法，還是高度特異性的免疫檢測法都可以如願以償。
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