蛋白能量比 的英文怎麼說
中文拼音 [dànbáinéngliángbǐ]
蛋白能量比
英文
protein caloric ratio- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 能 : 能名詞(姓氏) a surname
- 量 : 量動1. (度量) measure 2. (估量) estimate; size up
- 比 : Ⅰ動詞1 (比較; 較量高下、 長短、距離、好壞等) compare; compete; contrast; match; emulate 2 (比...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
- 量比 : fnc
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In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Following the development of the study in extracellular matrix ( ecm ), people find that the ecm is composed of glucoprotein and glycosaminoglycans, and it ' s components induct the cell differentiation in tissues and organs, in turn, it can modulate the function of these
隨著人們對生物體細胞外基質認識的深入,明確了它是由不同含量的糖蛋白以及糖胺聚糖組成的按一定比例和結構建成的復雜的有機的統一整體。它的各組成成份,分別誘導分化組織、器官內的不同細胞,通過調節各細胞的生長、分化進而調整組織、器官功能。It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖,最佳比例為2 : 3 ,最佳濃度為1 . 6 ;加入其它碳源時有助於菌株穩定產酶; 100ml搖瓶的最佳裝液量為15ml ;肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶,其中肌酸誘導產酶的效果最好,而肌酐和尿素不能誘導菌株產酶;誘導物肌酸的最適加入時間為接種培養12小時后,最適加入量為0 . 75 。Therefore, the structural modification of n - glycan and the functional changes of egf receptor ( egfr ) in different transfected cells were investigated. it was found that the 1, 6 glcnac branch on the n - glycans of immuno - precipitated egfr on gntv - s / h7721 cells was increased while it was reduced in gntv - as / h7721 cell despite the unaltered expression of egfr protein
因此本文對不同轉染細胞egf受體( egfr )的糖鏈結構及功能的改變作進一步研究,結果發現,不同轉染細胞中,免疫沉澱egfr的蛋白量沒有改變,但gntv - s h7721細胞egfr上n -糖鏈中1 , 6glcnac分支比mock細胞增多,而gntv - as h7721細胞相反。Abstract : this paper researches into the complex adhesive , which was mainly made by the soy protein isolate ( spi ) and polyvinyl alcohol or polyvinyl acetate. different composition of protein and polymers can perform different characters, together with other ingredients. the factors, which are related to the first adhesive strength and adhesive strength, are mainly studied and discussed. the first adhesive strength of the complex adhesive is related to the content of spi, and the adhesive strength is related to the content of the complex adhesive. on the basis of the researching, we can make the plant fibre box for food in the future
文摘:對大豆分離蛋白?聚乙烯醇、大豆分離蛋白?白乳膠復合膠粘劑進行了研究,採用不同混合比例及添加其他助劑,得到較好性能和可生物降解的復合膠粘劑,為製造一次性植物纖維快餐盒打下基礎.主要研究了影響這種復合膠粘劑粘接木塊的初粘力(剪切和拉伸強度)和粘接強度的因素,實驗表明: 9 . 2 %濃度的大豆分離蛋白的初粘力(剪切和拉伸強度)優於10 %濃度的聚乙烯醇膠和33 %濃度的白乳膠;大豆分離蛋白復合膠粘劑的初粘力主要與大豆分離蛋白含量有關,其最終粘接強度與膠液固含量正相關To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression
蛋白免疫印跡分析的結果表iv明,當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時,細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平,與未經抗體處理時相比發生了變化(或升高或下降) ;而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少,使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。This product nutrition ingredient structure is good, the widespread use feed small wheat bran, the fine rice bran, the corn and the common distiller ' s grain powder protein content high, the crude fiber germany content is more moderate than, front several kind of feeds belong to the energy feed and the coarse fodder, but this product belongs to the protein feed, specially the amino acid content is other several kind of feed 3 ~ 5 times, moreover real protein proportion big ( amino acid total content / thick protein content = 73. 6 % ), must the amino acid type multi - content be high
該產品的營養成分結構好,比廣泛使用的飼料小麥麩、精細米糠、玉米和一般酒糟粉的蛋白質含量高、粗纖維德含量適中,前面幾種飼料屬于能量飼料和粗飼料,而該產品屬于蛋白質飼料,特別是氨基酸含量是其他幾種飼料的3 ~ 5倍,而且真蛋白的比例大(氨基酸總含量/粗蛋白含量= 73 . 6 % ) ,必須氨基酸的種類多含量高。The optimum nutrient requirements and energy - protein ratio in formulated feed in juvenile schizothorax prenanti
齊口裂腹魚幼魚飼料中營養素適宜含量和最適能量蛋白比的研究For example, a protein that breaks down glucose so the cell can use the energy stored in the sugar, will have a shape that recognizes the glucose and binds to it ( like a lock and key )
比如用來打破葡萄糖以便細胞可以從中吸取能量的蛋白質將會呈現一個能識別並附著在葡萄糖上面(就像鎖和鑰匙)的形狀。The system can, for example, use family history and elevated levels of a particular protein in the blood to work out who should undergo a biopsy to look for prostate cancer
比如說,透過家族病史和血液中某種特定蛋白質含量的提升,系統能判斷出病患是否需要接受診斷攝?腺癌的活組織切片檢查。It has not only many advantages of the prokaryotic expression system such as growing quickly, manipulating easily, strong power to express and control foreign protein, but also the ability to make foreign protein modified in post - translation. so pichia pastoris gene expression system is more appropriate to express many eukoryotic proteins than prokaryotic system and has attracted more and more people " s attention on it
它不僅具有原核表達系統生長快速,操作簡單,外源基因表達量大並受到嚴格調控等特點,還具有真核生物特有的蛋白質翻譯后修飾的功能,表達真核生物蛋白質具有原核表達系統無法比擬的優越性。This domain receives the signal from the sensor partner in bacterial two - component systems. these results indicated that the protein of the orf4 works as atpase function involved in the synthesis of magnetosome or takes part in the signal transduction relate to the promotion of magnetosome biosynthesis
通過對orf4編碼蛋白同源比較和功能分析,推測orf4編碼蛋白可能的功能有兩種:具atpase活性,在磁小體合成過程中為鐵轉運提供能量;或參與磁小體合成啟動過程中的信號轉導。Cardinal ) were determined. rose flowers had high contents of protein, vc, dietary fibre, polyphenols and flavonols, high proportion of essential amino acid in total amino acid and high proportion of linoleic acid in total unsatured fatty acid. the great application value of rose flowers in the field of functional food was discussed
以紅衣主教為材料,測定了玫瑰花的營養成分和多酚、黃酮等活性成分含量,發現玫瑰花中蛋白質、 v _ c 、膳食纖維和多酚、黃酮的含量較高,氨基酸中必需氨基酸所佔比例以及不飽和脂肪酸中亞油酸所佔比例較高,表明玫瑰花在功能食品領域具有廣闊的開發前景。Different resolution pixels have a series of transmission coefficient, according to this, feature of biological specimen, we device a protein model with several differnt high steps
摘要按照生物樣品分辨單元具有多種透過率的特點,設計了一個多襯度的臺階狀蛋白質模型,根據曝光量與襯度,信噪比,抗蝕膜性能的關系,確定了曝光量。It shows that the longest band ( 675bp ) has lower homology with flower - timing genes, but there is 80 percentage of homology with pectinesterase during citrus development, in 184 score of hsp. it is clear to see a functional domain by modeling three - dimension structure of the protein
結果發現分子量最大的片段(長675bp )與成花計時基因同源性較低,而與植物發育過程中的果膠酶基因具有80同源性,而且局部片段對比分值達184 ;通過蛋白質三維結構預測發現,它具有明顯的功能結構域。Even better ? by sticking with healthful toppings like veggies and lean protein ( grilled chicken is a good choice ), a medium slice will set you back only 200 to 250 calories
這還不行?那你就堅持吃健康的澆頭,比如蔬菜和瘦肉蛋白(烤雞是不錯的選擇) ,一塊中等量的比薩只能為你減少200到250卡路里的熱量。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。Vegan and vegetarian diets therefore can be balanced by combining foods that together provide the nine essential amino acids in these correct proportions, or by including adequate amounts of soy protein in the diet
素食主義者和吃素的人可以通過把含有不同必需氨基酸的食物按照適當的比例搭配食用來滿足機體對各種必需氨基酸的需求。增加膳食中大豆蛋白的數量也能達到同樣的目的。分享友人