蛋白計 的英文怎麼說
中文拼音 [dànbáijì]
蛋白計
英文
albumimeter-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The results showed that : adding tryptone, soy peptone. beef extract, com extract and cys - hcl to jaj could obviously promote the growth of blm and bbm ; by the orthogonal experiment of three elements and three levels, a satisfying jaj compound medium was acquired which included corn extract ( 0. 3 % ), soy peptone ( 0. 05 % ) and cys - hcl ( 0. 025 % ). nextly, after establishing a selective bifidobacterium medium, the effects of jaj on the growth of bifidobacteria in vivo were studied, using healthy mouse of kunming species as experimental animal
研究了以菊芋汁為主要原料的雙歧桿菌培養基，大量試驗結果表明，在菊芋汁中添加胰蛋白腖、牛肉膏、大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽等成分，對雙歧桿菌有明顯的促進生長作用；利用大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽設計了三因素三水平的正交試驗，確定了菊芋汁復合培養基的優化配方：菊芋汁+ 0 . 3玉米漿+ 0 . 05大豆蛋白腖+ 0 . 025半胱氨酸鹽酸鹽。After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library
在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後，從中選出了17條可能編碼抗菌肽，溶菌酶，凝集素、絲氨酸蛋白酶及其抑制劑，抗凍蛋白等蛋白質的序列，以此為依據設計引物，在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。Compared to control, retention times of digesta in whole alimentary tract of immunized animals inc reased by 20 hours ( to use cumlative excretion of 5 % marker as reference ). immunoneutralization of ss significantly augmented activities of digestive enzymes ( proteolytic, trypsin, chymotrypsin, amylase ) in pancreas and the small intestine ( control and immunized animals were 1693. 67unit / g, cp, 2728. 33 unit / g, cp, 3055. 50 unit / g, cp, 12. 9x106 unit / g, cp ; 2. 57x 102unit / g, cp, 1. 20x103unit / g, cp, 1. 12x 103unit / g, cp, 2. 98x 107unit / g, cp ft 2451. 33 unit / g, cp, 2904. 17 unit / g, cp, 4279. 33 unit / g, cp, 20. 61 x 106 unit / g, cp ; 6. 45 x 102unit / g, cp, 2. 53 x 103unit / g, cp, 1 - 83 x 103unit / g, cp, 5. 77 x 107unit / g, cp, respectively, p < 0. 05 or p < 0. 01 )
12ng ml ， 0人su vg ，各指標比較均差異不顯著， p 0刀5人兔疫組動物的食糜消化道滯留時間明顯增加（以指示劑累計排出50為標準，兔疫組較對照組大約增加20小時） ，與此同時， ss免疫中和也提高了胰腺和消化道各種消化酶的比活力（對照組和免疫組胰腺，小腸食糜總蛋白酶，胰蛋白酶， 」糜蛋白酶和澱粉酶比活分別為1693石7unit g ， cp ， 2728The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr
由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。The sds - page results showed the fusion protein was efficiently expressed in the soluble form. 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli. k12
三、對以上表達的融合蛋白進行初步純化以及利用enterokinase切割融合蛋白，並進行抗菌活性檢測，結果表明所設計的cmiv突變體具有抗菌活性。We launched an integrated designing experiment to isolate, purify and identify a protein from a tissue, we also set up a designing experiment isolating - globin from serum
開設蛋白質分離、純化、鑒定的綜合性實驗，並開設了設計性實驗從血清中初步分離-環蛋白，由學生自己查找原理和方法、自己設計實驗步驟、自己動手實施，並且用獲得的樣品進行精細分離和鑒定。A team at the massachusetts institute of technology engineered a long tubular virus called m13 to sheathe itself in proteins that glom onto cobalt and gold atoms
美國麻省理工學院的團隊把一種叫做m13的長管狀病毒拿來設計，使得它以蛋白質鞘包覆自己，並讓鈷原子與金原子附在蛋白質上頭。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。Methods : after cultured scs combined with fn were grafted into hemi - resected lumbar spinal cords of adult rats, we used surface recording of spinal cord evoked potential ( scep ) and image analysis system to compare the latent period of scep and count the axon number between the therapeutic group and the control group
方法：將體外培養的雪旺細胞結合纖連蛋白植入大鼠半切損傷的脊髓內，用脊髓誘發電位及圖像分析方法比較治療組和對照組誘發電位潛伏期，再生軸突計數及二者間的關系。Lenis explains the basic concepts of how to create non - toxic meals ( this type of cooking is sometimes called slow food or low temperature cooking ) then goes to the kitchen where everyone gets a hand on experience of how to make delicious foods that are quick, easy, and healthy. you will also learn the basics of nutrition including eating for blood sugar and metabolism regulation ( key for weight control ), protein and carbohydrate requirements, healthy fat intake, vitamin and mineral requirements, calorie counting methods, understanding the effects of alcohol, identifying sources of toxins, and many important subjects for healthy nutrition
您也會學習到有關于營養的基本知識，包括能控制體重的血糖和新陳代謝機制的飲食方法、蛋白質和碳水化合物、健康脂肪、維他命及礦物質的正確攝取量，和計算熱量的方法，並且能夠理解酒精對人體的影響、認識毒素的來源還有許多有關于營養健康的議題。Now the apo1lo project, the manhattan project, as we11 as the hman genome project required the most significant cooperation among the scientists a11 over the world, do it together and share the know1edge as well as the profit respectiyely, it has become the famous princip1e of hgp. the topics mainly included in this dissertation : 1. the first c1ass of science and technology usua1ly will become the first class of industry
通過對基因理論和基因技術發展過程的考察，可以明顯地看出：現代的生物高新技術呈現出逐漸加速的與其他學科相互交叉、相互借鑒、相互融合的趨勢；如，基因技術與蛋白質技術與信息技術的交叉和混合生長? ?基因組學、生物信息學和生物晶元技術以及設想中的生物計算機技術。Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %
本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。He is currently studying product design at hfg offenbach in germany
他目前正在研究在產品設計蛋白原德國奧芬巴赫The predicted secondary structure of the protein displays multiple putative transmembrane domains
1 。計算機軟體分析表明，其編碼的蛋白具有多次跨膜樣結構域。J mol biol., 1992, 224 : 53 - 63. 26 abe h, aiba h. differential contributions of two elements of rho - independent terminator to transcription termination and mrna stabilization. biochimie, 1996, 78 : 1035 - 1042
通過計算，我們預測到266個不依賴終止子，其中包括232個蛋白編碼基因， 12個trna基因和3個rrna基因，約17 %不依賴終止子位於操縱子的末端。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。It is estimated that the protein, ricin, found in the castor bean plant is as much as 6, 000 times more poisonous than cyanide
據估計，蓖麻子豆中找到的一種名為蓖麻毒素的蛋白質，所含的毒性物質比氰化物多出了6000倍。Based on dyeing methods of sappanwood, application of sappanwood as natural plant dyestuff in natural silk, soybean - protein fibre, ramie and wool fabric was listed, implying some problems of natural plant dyestuffs and future application of sappanwood dyestuff was foreseen
摘要在對蘇木染色機理分析的基礎上，簡要例舉了蘇木作為天然植物染料在真絲、大豆蛋白纖維、苎麻以及羊毛織物中的應用，指出了天然植物染料存在的問題，對蘇木染料的應用前景作了樂觀的估計。分享友人