融合細胞培養 的英文怎麼說

中文拼音 [rónggěxìbāopéiyǎng]
融合細胞培養 英文
fusion cell culture
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation

    簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。
  2. Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each

    分別取第5代和第13代的mscs ，以8x10 cm 『濃度接種於六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，並在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而後換成無血清的培養液。
  3. Research into stock and wallflower protoplast and electrical cell fusion

    紫羅蘭與桂竹香原生質體培養及電激細胞融合的研究
  4. Different from mammals, the early embryos of fish can not be preserved for the long period at the very low temperature ( - 196 ). therefore, three methods were usually applied to cryogenic preservation of the fine and rare species of fish : 1 ) perserving fish spermatozoon in cryogenic condition. researchers have had systematically studied on this technique for many years, and this technique has been utilized in application and made a lot of effects ; 2 ) combining with the techniques of cell engineering ( nuclear transplantation and electric fusion etc. ), and through the process of culturing histiocyte of fish, cryopreservation and re - culture after thawing, carrying out somatic cell breeding of fish. the past studies showed that the nucleolus of somatic cells of fish have totipotency

    多年來，國內外學者對各種魚類精液的冷凍保存進行了大量的系統研究，目前這項技術已達到實用水平，並日益發揮作用；二是對魚類培養的組織細胞冷凍保存，通過魚類細胞的培養、超低溫凍存、解凍后再培養過程，結合細胞工程技術（如核移植、電融合等）進行體細胞育種；大量的研究結果表明魚類體細胞核具有發育的全能性，隨著細胞培養技術、細胞工程技術日益發展成熟，完全具備實現魚類物種種質長期保存的理論基礎和技術條件。
  5. Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall, and agrobacterium / " / z / zogerae. s ' - transformed cell line of medicago saliva l. using protoplast fusion by peg method

    通過peg誘導原生質體融合和雜種細胞的篩選培養，首次得到沙打旺（ + ）紫花苜蓿的屬間體細胞雜種。
  6. After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed

    建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。
  7. Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body

    將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。
  8. The secretive expression of scfv fusion proteins were confirmed by sds - page and western blot analysis in cell split products and condensed supernatant

    建系細胞裂解產物和濃縮的培養上清經sds page及westernblot分析，證實了scfv融合蛋白的分泌性表達。
  9. We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml

    無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘，吹打b人兩個培養瓶，置人37 j coh飽和濕度條件下培養，隔日更換培養液，當細胞達到70融合時，傳代。
  10. Results : the primary cells formed a monolayer in 18th day. von - kossa staining presented mineral deposition after the cells had been cultured 30 days in calcified solution. conclusion : bmsc can be isolated and cultured easily

    結果:原代細胞培養第18d細胞融合成單層，長滿整個培養皿;倒置顯微鏡下觀察，礦化條件下，細胞培養30天， von一kassa染色顯示有局灶狀鈣鹽沉積。
  11. Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained

    方法：基因工程p24抗原免疫小鼠4次，歷時2個月，取脾細胞與骨髓瘤細胞株sp2 0 ，用peg融合， hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞，三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。
  12. After 15h of maturation. 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended. secondly, strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium

    第一次減數分裂期小鼠卵母細胞經化學去核后，去除透明帶，並胎兒成纖維細胞粘合、電融合併srcl2激活，培養于微孔中。
  13. 1 ( + ) / hsblys was trans fected into cos - 7 cells with the calcium phosphate method and the lipofection method. we cultured the recombined cos - 7 cells at optimal condition and harvested cells every 24h. in order to find out the optimal expressive condition, we lysed the cells, extracted the protein, and measure the content of the hsblys

    此外，用構建的融合表達載體pcdna3 . 1 （ + ） hsblys轉染真核宿主細胞cos - 7 ，然後在合適的培養條件下進行表達，取不同表達時間（ 48h 、 72h和96h ）的細胞破碎后進行分析鑒定，找出最合適的表達時間及其他條件。
  14. The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell

    表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。
  15. Preparation requires isolating the patient ' s white blood cells and then separating out a mix of dendritic cells and precursor cells still undergoing maturation ( both types may produce an immune reaction )

    其準備功夫包括先分離病人的白血球，然後從中分離出樹突細胞以及未成熟前驅細胞的混合物（這兩種細胞都可能產生免疫反應） ，再與融合蛋白一起培養40個小時，之後才注射回病人身上；這個步驟一共要重復三次。
  16. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  17. Compared with the cytoplasts matured in the maturation medium directly for 16 ~ 18h, the fusion rate, cleavage rate and blastocyst development of reconstructed embryos from oocytes cultured in 2mmol / l 6 - dmap for 3 - 5h prior to ivm did not differ significantly from that of reconstructed embryos from oocytes matured in the maturation medium directly for 16 ~ 18h

    水牛卵母細胞先經水牛卵母細胞孤雌激活與體細胞核移植的研究6 dmap抑製成熟培養3 sh ，再在正常成熟培養液中成熟培養16 18h 。結果發現，與直接在正常成熟培養液中成熟培養16 18h的卵母細胞相比，其融合率，分裂率和囊胚發育率均無顯著影響。
  18. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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