融合肽 的英文怎麼說
中文拼音 [rónggětài]
融合肽
英文
fusogenic peptide-
The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr
由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。Moreover, they have hi common the localization in the golgi apparatus, the topology of type ii membrane proteins, and the transfer of galactose from udp - galactose onto the c4 - hydroxyl group of glcnac residues
用iptg誘導表達的獲得- 1 ， 4 - gt與gst （谷胱甘肽轉移酶）的融合蛋白，經westernblot驗證與預想的一致。To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。Thinking of the cptl is small and its poor stability, a gst fusion expression system of pgex was used to improve the immunogenicity of cptl
考慮到cpti為小分子多肽，穩定性較差，為增加cpti蛋白的免疫原性，本研究採用了cpti與谷胱甘肽gst融合的pgex表達系統。One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。Da - kang world will be a very good sign specialty products group cordyceps polypeptide structure into markers, sport group in connection with the world and the phonetic alphabets, we demonstrate the enterprise, but also highlights the nature of the enterprise
標志很好的將大康天下集團的特色產品蟲草多肽的形態結構融入到標志中來，並結合大康天下集團的拼音字母，既展示了企業，也突出了企業的性質。Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。To circumvent these deficits, novel antigen - delivery systems utilizing cytokine gene - modified tumor cells and dc or fusion of dc with tumor cells have resulted in induction of antitumor immunity. however, this u approach is difficult in some cases ( for example in breast cancer ) because only rarely has it been possible to isolate enough viable tumor cells from an individual to prepare the vaccine
為了克服上述缺陷，有學者採用滅活的腫瘤細胞、腫瘤抗原提取物（包括腫瘤細胞裂解物、 it na和洗脫肽等）沖擊致敏dc或將腫瘤細胞與dc融合后再回輸體內以激發機體的抗腫瘤免疫應答，也取得了較好的療效。Methods employed to prepare vaccine include rumor cells genetically modified with cytokines, costimulatory molecules and tumor antigenic peptides, dendritic cells ( dc ) primed with tumor antigens in vitro or genetically modified with tumor antigens, or fusion of tumor cells with antigen presenting cells ( apc )
現有的比較受到關注的制備腫瘤疫苗的方法有各種細胞因子、共刺激分子、腫瘤抗原肽等基因修飾的腫瘤細胞疫苗；體外抗原致敏的或腫瘤抗原肽基因修飾的樹突狀細胞疫苗；腫瘤細胞與抗原遞呈細胞融合疫苗等。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious
前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。The two fragments were ligated to a whole fusion peptide gene, next was ligated with two adapters which have the same cohesive end ( atg / tac ), respectively
首先將其連接成融合肽的完整基因，然後分別與含有相同粘性末端（ atg / tac ）的ecor前接頭和sal後接頭連接。Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression, a novel mass - production method is proposed. it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
為提高抗菌肽的表達，設計在抗菌肽基因的n端融合一段編碼酸性短肽的片段作為對抗菌肽前體的模擬，以減輕表達產物對宿主的毒性；同時通過含有酶切位點的接頭將該融合肽基因以同向串連的方式連接成多拷貝基因。Then the product was purified by glutathione sepharose 4b chelation affinity chromatography, and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application
表達產物經谷胱甘肽瓊脂糖4b親和層析純化后，得到了純度較高的gst - gnrh trs融合蛋白。為進一步科研和實際應用奠定基礎。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Two expression systems were used, one of which was qiaexpress system, the other glutathione s - transferase ( gst ) gene fusion system
）系統和谷胱甘肽s ?轉移酶（ gst ）基因融合表達系統。The scfv genes were then inserted into the prokaryotic fusion protein expression vector ptat - ha and expressed in e. coli bl21. after the expression products were purified by ni - nta colume, their activities were analysed
將獲得的不含前導肽的單鏈抗體基因克隆入ha和6his融合的原核表達載體ptat - ha ，在大腸桿菌bl21中進行表達，分析其表達情況。Objectives : to amplify the cdna encoding the 45 amino acid containing natural form of human p - defensin 3 and clone it into the prokaryotic expression vector. then express the recombinant protein in e. coli and purify it in order to get active recombinant h d - 3 fusion protein
目的：擴增編碼h d - 3成熟肽的cdna ，將其構建於原核表達載體后利用大腸桿菌誘導表達，最終獲得具有抗菌活性的h d - 3融合蛋白。分享友人