融合蛋白質 的英文怎麼說

中文拼音 [rónggědànbáizhí]
融合蛋白質 英文
fused protein
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  • 蛋白質 : [生物化學] protein; proteide （舊稱「朊」, 由多種氨基酸結合而成的高分子化合物）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Its content was about 9. 8 % among total cell protein by gene genius bio imaging system. the fusion proteins were found largely in an insoluble inclusion bodies. the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105

    經工ptg誘導，重組質粒在點co力『 blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。
  3. 97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found

    將含有重組質粒phn的菌株dh5在37條件下培養，以濃度為0 . 6mmol / liptg誘導，重組質粒n基因phn融合蛋白獲得了表達：經sds - page ， western - blot試驗，確定其表達的融合蛋白產物大小為預期的47ku 。
  4. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  5. Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins

    Coltblzi生產融合蛋白，重組后的dna序列包括一個pgex質粒，依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下，而枯啟動子可由乳糖的類似物i剛來誘導它表達。
  6. Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids

    Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。
  7. This has allowed the development of a balanced - lethal system for the expression of heterologous antigens in vaccine strains using vectors containing the wild - type asd gene from s. typhimurium and asd mutant salmonella hosts

    Sds一page和westem一blot檢測表明，菌苗侯選株能夠分別表達出相對分子質量為64kda的尿素酶b蛋白與77kda的尿素酶b和熱休克蛋白a的融合蛋白。
  8. An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells

    利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。
  9. The expressed protein was purified and used to generate antibodies in rabbits

    被表達的融合蛋白質經純化后，注射免疫家兔獲得抗體。
  10. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  11. Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5

    Sds - page分析表明，重組質粒pet - 32a （ + ） - igf -在iptg誘導下表達分子量約30kda的融合蛋白，但其表達量不高，約為菌體總蛋白的10左右；重組質粒pet - 30a （ + ） - igf -在iptg誘導下表達分子量約14kda的重組蛋白，融合蛋白表達量約占菌體蛋白總量的35 。
  12. The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5

    將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。
  13. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  14. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  15. Second, a prokaryotic expression construct, obtained from invitrogen transformed into prokaryotic and induced to express vp1 protein. the expressed vp1 fusion protein was purified by affinity chromatography using glutathione - agarose resin and used in elisa and western bolt analysis as the antigen. the elisa and western blot results showed that the anti - fmdv antibody was elicited specifically against vp1 antigen

    第二，為了得到抗原蛋白，將vp1的原核表達質粒pgex - 4t - vp1轉化入大腸桿菌bl21中，經iptg誘導，裂解細胞後用瓊脂糖珠進行純化，用elisa和westernblot進行檢測，結果表明誘導表達出所需大小的融合蛋白。
  16. The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell

    表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。
  17. The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23. 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )

    Rt - pcr分析確證重組質粒在大腸桿菌中能正確轉錄； sds - page分析證明誘導重組質粒表達了融合蛋白，分子量約為50kd ，與預期的26kd的gst帶和23 . 6kd的草魚生長激素基因編碼蛋白質構成的融合蛋白大小一致。
  18. The vp6 gene was subcloned from recombinant plasmid pmd18 - t - vp6 into expression plasmid pet - 30a. the recombinant plasmid pet - 30a - vp6 was transformed into e. coli bl21 ( de3 ) and induced with iptg. not only a fusion protein about 45ku as we expected was found but also several smaller polypeptides were observed

    從重組質粒pmd18 - t ? vp6上將vp6基因亞克隆到表達載體質粒pet - 30a ，轉化大腸桿菌bl21 （ de _ 3 ） ， iptg誘導以後表達出預計的45ku的融合蛋白，同時還有一些小的蛋白表達出來，光密度掃描對表達產物進行初步定量表明， 45ku融合蛋白占菌體總蛋白的26 . 5 。
  19. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  20. These suggest the bsp proteins and its homologous proteins are ubiquitous in mammals and must have some physical significances. in our research, to find and study the important role of bsp proteinsand their related proteins in the development of zygotes and to find the new gene related to bsp, we cloned a cdna gene sequence related to bsp proteins and make it express in eukaratic cells. the fusion protein acquired are purified are used to deduce its biological functions

    而在我們的實驗中為了發現和研究bsp及其相關蛋白在受精卵的發育中的重要作用，尋找bsp相關的新基因，克隆了一個與bsp蛋白相關基因的山一na序列並將該基因編碼的蛋白質在原核細胞中進行了表達和純化，將純化后的融合蛋白作了一些生物學功能方面的初步工作，發現新蛋白對tpk有一定的抑制作用，而tpk是細胞生長和代謝中十分重要的關鍵酶，這對于進一步研究新蛋白的生物學功能具有十分重要的意義。
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