表位鑒定 的英文怎麼說

中文拼音 [biǎowèijiàndìng]
表位鑒定 英文
epitope identification
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 鑒 : Ⅰ名詞1 （鏡子 古代用銅製成） ancient bronze mirror2 （可以作為警戒或引為教訓的事） warning; objec...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 鑒定 : 1 （評語） appraisal （of a person s strong and weak points） 2 （評定） appraise; identify; auth...
  1. Bloom in youth s smart blue oxford suit with white vestslips, narrowshouldered, in brown alpine hat, wearing gent s sterling silver waterbury keyless watch and double curb albert with seal attached, one side of him coated with stiffening mud

    隨后，他在信里附了一朵火絨草，說是為了向我表示敬慕，特地從山丘上采來的。我請一位植物學專家給鑒定一下。
  2. Declare the ginseng that stipulates disease is planted to keep staff, should by via treating hospital disease director ou fills in " fine disease of regulation of insurance of primary medical treatment plants worker of the town that start city outpatient service examines and approve a watch ", add the material such as result of him anamnesis, diagnostic proof and the closest examination, assay, after the sectional examine and verify that via the hospital responsible and primary medical treatment is sure to manage, by place unit cure signing up for city protects agency orgnaization, appraisal of classics expert group accords with condition and cure to protect agency orgnaization to examine and approve assentient, send " formulary disease is planted special anamnesis ", formulary disease is enjoyed to plant inside significant time of the regulation pay of medical treatment insurance

    申報規定病種的參保人員，應由經治醫院病區主任填寫《嘉興市城鎮職工基本醫療保險規定病種門診審批表》 ，附本人病歷、診斷證實及最近的檢查、化驗結果等材料，經醫院負責基本醫療保險治理的部門審核后，由所在單位報市醫保經辦機構，經專家組鑒定符合條件和醫保經辦機構審批同意者，發給《規定病種專用病歷》 ，在規定的有效時間內享受規定病種醫療保險待遇。
  3. The monoclonal antibodies ( mab ) specific for the nucleoprotein of prrsv were used to select a phage random heptapeptide library

    採用噬菌體隨機7肽庫對單克隆抗體ge3識別的抗原表位進行了鑒定。
  4. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  5. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  6. Present methods generally based on the statistics of earthquake damage, expert experiences, theory analysis and experimental researches have obvious advantages, disadvantages and certain scopes of application ; ( 2 ) different prediction methods should be adopted against different building conditions, sites, intensity and experiences etc to predict earthquake damage of buildings for prospective accuracy, dependability and availability ; ( 3 ) earthquake damage matrix, which is the foundation of earthquake damage prediction, of 7 kinds of building in the urban areas of zhangzhou city under intensity 6 to 9 has been set up. the damage conditions of different buildings under different intensity are as followings : all kinds of structures are basically intact under intensity 6 ; the reinforced concrete structures are basically intact under intensity 7, but other kinds of structures are destroyed slightly ; the reinforced concrete structures are still basically intact while other kinds of structures are destroyed intermediately under intensity 8 ; the reinforced concrete structures are destroyed slightly, single - story factories and open houses are destroyed intermediately and other kinds of structures are destroyed seriously under intensity 9 ; ( 4 ) the results of earthquake damage predicting of buildings embody the damage when earthquake happens in the future. thus, further identifications and reinforcements should be considered to buildings that will be destroyed intermediately or more under the earthquake with 10 % exceeding probability in future 50 years ; ( 5 ) the direct economic losses caused by damage of buildings resting with the area, structural type, intensity and damage of all kinds of buildings are the main part of the losses of the city in an earthquake ; ( 6 ) the direct economic losses increased progressively toward high intensity by 2 or 3 times

    基於上述研究，得出的主要結論有：建築物震害預測是一個模糊的、系統的、復雜的問題，現有的方法很多一般都是以震害統計規律、專家經驗、理論分析和試驗研究為依據，有其自身的優缺點和一定的適用范圍；應針對不同的建築物條件、場地條件、地震強度和已有經驗等，採用不同的預測方法進行建築物震害預測，以使預測結果達到預期的精確性、可靠性和可操作性；建立了漳州市區7類建築物在6度9度地震作用下的震害矩陣，成為指導抗震防災的重要依據，各類結構的震害情況表現為： 6度地震作用下各類建築物基本完好； 7度地震作用下除鋼筋混凝土結構基本完好外其餘以建築輕微破壞為主； 8度地震作用下鋼筋混凝土結構仍以基本完好為主而其餘建築以中等破壞為主； 9度地震作用下鋼筋混凝土結構以輕微破壞為主，單層工業廠房和空曠房屋以中等破壞為主，其餘建築以嚴重破壞為主；建築物的震害預測結果體現了未來地震來臨時的震害程度，在編制漳州市區抗震防災規劃時，對于遭遇50年超越概率10的地震影響發生中等以上破壞的建築物應考慮進行抗震鑒定和加固；由建築物的破壞所造成的直接經濟損失是城市地震經濟損失的主要部分，重慶大學碩士學位論文中文摘要其主要與建築物總面積、結構類型、地震烈度和各類建築物的震害程度有關;不同烈度造成的直接經濟損失按2一3倍向高烈度方向遞增，漳州市區直接經濟損失由6度至9度的比例關系為1 : 2 . 8 : 8 . 6 : 22 . 8 ;遭遇基本地震設防烈度（ 7度）時，漳州市區直接經濟損失約4 . 5億元，無家可歸人員約40000人，且以磚木結構和多層磚混結構的震害損失最大;地震造成的人員傷亡主要與建築物倒塌及嚴重破壞的程度和總面積以及震時的建築物室內人數密切相關，地震時無家可歸人員主要與住宅倒塌、嚴重破壞及中等破壞的程度和總面積以及城市人均居住面積密切相關。
  7. The method of exterior measuring shore beacon has been put forward by using evaluation precision of original azimuth of missile aiming on the background of test for the precision of missile aiming " xx " underwater to surface missile on the sea, established math model and analyzed main parameters of measurement, principle, calibration and azimuth calculating for method of exterior measuring shore beacon was discussed

    對國內主要代表型號的導彈瞄準系統進行了比較詳細的綜述。文章是以「 xx 」潛地導彈海上瞄準精度試驗為背景，給出了一種外測岸標的方法來評估艦載導彈武器系統方位瞄準精度的鑒定方法，適用於潛地導彈初始方位瞄準精度的鑒定。
  8. The results suggest : dna samples that were extracted using the modified ctab method were successfully amplified by rapd ; rapd technique showed some potential to be useful in determining relationship between species within the two genera. the taxonomic position of ? linza, which is problematic based in morphological classification, showed a closer relationship with ulva

    結果表明，應用改進的ctab法獲得的dna樣品可成功用於rapd擴增； rapd技術適用於這兩個屬種間關系鑒定；基於形態分類有爭議的緣管滸苔的分類地位在rapd分析中表現出與石蓴屬更近的親緣關系。
  9. Articles published more than 60, 15 excellent article prizes ; 4 translations ; 2 reviews ; 5 works co - ed : 4 prizes, methodology on pharmacological research for traditional chinese medicine associate - editor, won the nationl library prize, the first class prize of library for excellent science and technology in the whole nation, and the third class prize for progress in science and technology in the nation ; methodology on pharmacological experiment, won the frist class preze of library for excellent science and technology in the whole nation ; pharmaclolgy and clinical applications on chinese traditional patent famous recipe associate - editor, and english - chinese and chinese - english dictionary on traditional chinese pharmacology standing editor

    代表性的成果有"兔腦磷脂抗炎免疫藥理研究"為山東省自然基金項目首位，鑒定達國際先進水平，論文發表在中國藥理學通報和中國生化藥物雜志： "磷脂和葛根素防治心血管病實驗研究"為山東省教委課題首位，鑒定達國際先進水平， 2000年獲省教委應用技術成果二等獎"藥物體外吸收實驗裝置的改進和應用" 2000年獲省級優秀實驗技術成果二等獎首位"蝎毒心血管藥理研究" 1991年獲省科技進步三等獎二位。
  10. Through studying the etiopathogenisis and clinical manifestation we analyze the relationship between injury and disease. 6 cases of 16 have lumbar sacralization. 10 cases are attributed to lumbarization

    方法回顧性分析16例有腰椎骶化或骶椎腰化的被鑒定人，結合其病因、臨床表現探討與外傷的關系，以及如何準確定位椎體。
  11. Base on the definition of active fault and its meaning to engineers, this paper reviews some geological aspects and problems, such as the application of dating results in determination of active faulting ages, seismo - tectonics background studying on moderate - strong earthquakes, estimating earthquake recurrence from geological investigation, and probabilistic evaluation of the ground offset of active fault

    摘要本文在對活動斷層的工程涵義進行了簡要分析后，討論了斷層物質測年的工程應用進展及其斷層活動時代鑒定問題，並就如何加強中強地震發震構造判別、應用地震地質資料評估大地震年平均發生率、以及活動斷層地表斷錯位移的概率評價等問題作了初步探討。
  12. At present, in an attempt to stimulate specific antitumor immunity, experimental models and clinical studies are currently evaluating the potent antigen - presenting capacity of dc combined with single or multiple tumor antigen epitopes. however, there are several problems in utilizing pulsing dc with synthetic immunodominant peptides from identified antigens. 1 ) the potential induction of tolerance ; 2 ) the need to determine the patient ' s hla haplotype, the limitation of therapy to patients whose tumors express defined specific tumor antigens in the context of the correct hla phenotype, the unavailability of peptides for all hla haplotypes ; 3 ) the lack of cd4 help cell - related epitopes for most antigens ; and 4 ) the ctl resulting from such protocols have a good in vitro capacity to kill peptide - pulsed target cells but only a modest capacity to kill tumor cells

    但是，學者們發現這一療法存在著如下的缺陷：單一ctl表位抗原肽的應用其抗腫瘤作用弱於多種腫瘤抗原的聯合應用，且有誘導免疫耐受的潛在危險，有時反而會促進腫瘤的生長；事先需對患者的hla單倍型進行鑒定，以確定ctl表位與hla單倍型是否匹配，目前尚缺乏能與所有hla單倍型相匹配的ctl表位，從而限制了這一療法的應用；這一療法所產生的ctl在體外能有效殺傷經腫瘤抗原肽共孵育過的靶細胞，但對腫瘤細胞的殺傷能力較弱：這種l表位抗原肽缺乏cd4汀h細胞相關的表位，因此，不能誘導有效的th細胞免疫應答。
  13. This study dealt with the identification and evaluation of 8 maize populations from tropical and subtropical regions under the ecological conditions in zhengzhou, henan. results showed that the significant delaying days to shed and days to silk, discoordinating for the development of male and female, higher plant and ear height, more leaf numbers, high barreness, worse performance of ear traits compared with the ck ( temperate population ) were found, which showed the tropical and subtropical populations could not be utilized directly under the ecological conditions in zhengzhou. by using selection and improvement the tropical and subtropical populations can be used to broaden the genetic base of temperate maize breeding, beause of the greater genetic variation and higher selection potential within each population. the different performances of the sensitivity to photoperiod were observed among different tropical and subtropical populations and different traits. the asi, time to silk, ear height, leaf numbers, grain yield per ear could be used as the good evaluation traits for identifying the sensitivity to photoperiod

    對8個熱帶、亞熱帶玉米群體在鄭州生態條件下的表現進行鑒定和評價，結果表明，熱帶、亞熱帶群體散粉、吐絲期顯著延遲，雌雄不協調，植株和穗位高、葉片數增多、空稈率高，結實性差，果穗性狀均明顯劣於溫帶對照，在鄭州生態條件下不能直接利用，但是群體內具有較大的遺傳變異和選擇潛力，可以用來拓寬現有玉米種質的遺傳基礎；不同熱帶、亞熱帶群體的光周期敏感性不同；不同性狀對光周期反應的敏感程度不同， asi 、吐絲期、穗位高、葉片數、穗粒重可作為鑒定光周期敏感性的較好指標
  14. Identification and characterization of neutralizing antigen epitope of hepatitis e virus clustered in genotype

    基因型毒株中和抗原表位的鑒定
  15. Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long - time expression of grf

    通過提取注射部位dna后進行pcr鑒定，可以確認原生質體介導的grf基因至少可以在受試動物體內滯留28天以上，為grf的長期表達提供了條件。
  16. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  17. In this project we use etching method x - ray transmission and tem observe and study the form mechanism of cell structure and linear structure ; use sem observe cell structure directly and evaluate effect to the electrical properties of substrate ; at last, use high resolution tem and eds to observe and identify the nature of microdefects

    發現幾乎所有高位錯密度的si - gaas單晶的表層都具有網路狀胞狀結構或系屬結構，首次對該胞狀結構和系屬結構的形成機制進行了研究；直接觀察微缺陷，配合eds （能量色散譜）鑒定si - gaas中微缺陷的物理本質，同時分析其產生原因，討論與位錯的相互作用。
  18. In he - 3 and de - 3, 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously. in he - 2 and de - 2, 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously, some of these different proteins are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction. it shown that gene expression have significant difference between diploid and haploid during embryos developing

    分別選取其中70個分辨好的差異蛋白質點進行原位胰蛋白酶酶解，酶解的肽片段用基質輔助激光解析電離分行時間質譜（ maldi - tofms ）得到肽質指紋圖譜，再在網上peptident軟體中搜索swiss - prot和trembl數據庫，初步鑒定了he - 3和de - 3上32個差異蛋白質點，其中包括16個表達量上有差異的蛋白質點（ p 0 . 05 ）和16個有無表達的差異蛋白質點，並且初步鑒定了he - 2和de - 2上25個差異蛋白質點，其中包括5個表達量上有差異的蛋白質點（ p 0 . 05 ）和20個有無表達的差異蛋白質點。
  19. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  20. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
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