裂解酶 的英文怎麼說
中文拼音 [lièjiě]
裂解酶
英文
hydroxymethylglutaryl coa cleavage enzyme hmg coa-
Effect of agitation rate on batch alkaline pectate lyases fermentation by bacillus subtilis
分批發酵堿性果膠裂解酶的影響The best studied inducible and repressible enzyme in phytopathogenic prokaryotes is endo - pectate lyase in e. carotovora
植物病原原核生物中研究得最清楚的是胡蘿卜軟腐菌的果膠酸內裂解酶。( 3 ) uv - b radiation increased the phenylalanina ammonia - lyase ( pal ) activity in leafy thallus of duckweeds, and as a consequence, flavonoids were accumulated. flavonoids synthesis induced by supplementary uv - b is an adaptive response of duckweeks under enhanced uv - b radition
( 3 )紫萍可以通過提高uv - b吸收物質如類黃酮合成酶苯丙氨酸氨裂解酶( pal )活性,促進類黃酮的積累來適應uv - b輻射的脅迫。This inactive product is cleaved by a converting enzyme, mainly in the lung but also in the kidney and brain, to an octapeptide, angiotensin ii, which is a potent vasoconstrictor that also stimulates release of aldosterone
這一非活性產物經轉換酶裂解,主要是在肺部但也在腎和腦部,成為八肽,即血管緊張素,這是和中強烈的血管收縮素,可刺激醛固酮的釋放。Optimum fermentation condition for producing alginase
海藻酸裂解酶的發酵條件優化Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease ( ppase )酶切,酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后,得到純度較高的的m - centrin 。Activity of phenylalanine ammonia - lyase ( pal ) was increased by uv - b radiation, and as a consequence, uv - absorbing compounds were accumulated. it was inferred from our results that the accumulation of uv - absorbing compounds such as flavonoids may be a common response for plants in resistant to enhanced uv - b radiation
在uv - b處理下,紫萍的苯丙氨酸氨基裂解酶( pal )活性上升,並導致其體內吸收uv - b的物質積累,推測類黃酮積累可能是植物抵禦uv - b逆境的一個共同效應。Purification and properties of polygalacturonic acid lyase from ramie degumming bacillus subtilis no. 16a
苎麻脫膠聚半乳糖醛酸裂解酶的純化及酶學性質A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。Lyase an enzyme that catalyzes the separation of two parts of a molecule with the formation of a double bond in one of them
裂解酶:一類能催化一個分子的兩部分分離,並在其中一個上形成雙鍵的酶。Hydroperoxides were produced from unsaturated fatty acid in oils catalyzed by lipoxygenase, then in the hydroperoxide lyase, the grassy green and leafy green aroma volatile aldehydes were made
摘要脂肪氧合酶催化油脂中的不飽和脂肪酸生成氫過氧化物,氫過氧化物再在裂解酶的作用下,生成具有青草香、青葉香香氣特徵的揮發性醛類物質。The basic approach of unsaturated fatty acid catalyzed and oxygened by enzyme, the preparation of lipoxygenase and production of hydroperoxides, the preparation of hydroperoxide lyase and cleaving of hydroperoxides were mostly discussed
主要論述了不飽和脂肪酸酶催化氧化的基本途徑,脂肪氧合酶的制備及氫過氧化物的生成,裂解酶的制備及氫過氧化物的裂解過程。Then, nap300 and 75 were fused in a similar way to phba gene encoding 3 - ketothiolase for a direct comparison. the result indicated that the two promoter ' s expression efficiency reached a peak at the same developmental stage of tobacco, which means they have the advantage of being used simultaneously for expressing different foreign genes in plant
將nap300 、 7s分別與phba基因(編碼3 -酮硫裂解酶)相連,在相似表達環境中對二者功能進行比較,發現兩個啟動子表達模式基本相同並在同一時期達到活性高峰,因此nap300可用於改善phb合成基因在植物體內的表達調控。Hydantoinase ( ec 3. 5. 2 ) hydrolyzes its substrate 5 ' - monosubstantial hydantoin to enantiomerical n - carbamyl - amino acids and in turn, the resulting product can be chemically or enzymatically converted into the corresponding optically active amino acids
海因酶( hydantoinase , ec3 . 5 . 2 ) ,是一類催化海因、 5 』 -單替代海因及其衍生物環酰胺鍵斷裂的酰胺水解酶。Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k. a 109bp fragment was amplified from the human genome dna. 2. in 166 han samples, 19 ( 11. 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
用紅細胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標本的人白細胞基因組dna ,並以此為模板成功擴增出預期的109hpmbl目的基因片段。Rt - pcr kit was from takara technology co. ltd. the sequence of the primer of pkb are shown below, together with all the sodn and saodn oligodeoxynucleotide were synthesized in takara technology co. ltd. pkb primers ; forward primer 5 ' cga gaa gcc gcg acc caa cac3 ' and reverse primer 5atg cac tac cgc cgg a3 "
有絲分裂前, cdcz / cy山b 、組蛋白h ;激酶的活性通過被weel和myt激酶磷酸化cdcz的1卜巧和thr14殘基而抑制。在有絲分裂期mpf可以被cdc25活化,這兩個位點被去磷酸化, cdc25是蛋白水解酶,可以使cdcz的t打巧脫離磷酸化。4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase
表達載體pbv - a 、 pbv - b和pbv - c經誘導表達,發現所表達的蛋白均為可溶性蛋白,沒有包涵體出現;蛋白經sds ? page分析表明,基因phaa表達的蛋白分子量為42kda ,與?酮硫裂解酶分子量大小一致;基因phab表達的蛋白分子量為26kda ,與乙酰乙酰coa還原酶分子量大小一致;基因phac表達的蛋白分子量為63kda ,與pha合成酶分子量大小一致。The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively
我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析,並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物,從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子,其在sds - page電泳中的分子量約為98 . 5kda 。B - actin primers : forward 5tcc tat cgg cct cct cct ac 3 " and reverse 5aga atg tag tcc gcc agg tc 3 ", designed for 200bps. saodn oligodeoxynucleotide of pkb : stac agc ctg aga gga cct acc tg3 "
在有絲分裂期, edc25經歷216位ser磷酸化同時其水解酶活性增強,活化的mpf磷酸化並活化edc25 ,形成正反饋,結果進一步活化mpf前體,活化的mpf促進細胞周期g2 / m期轉換。Protein degradation is one of the major regulatory processes that allow the adaptation, repair, or removal of mutated or damaged thylakoid proteins during environmental or developmental changes. intracellular proteases play an essential role in the turnover of intracellular proteins
在光合作用中,葉綠體類囊體膜蛋白復合物進行光能的吸收、傳遞和轉化,水的裂解及光合磷酸化等,這些膜蛋白的周轉更新需要蛋白酶的作用。分享友人