親和偶聯 的英文怎麼說
中文拼音 [qīnhéǒulián]
親和偶聯
英文
affinity coupling-
In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。Although hippocampal neurons and astrocytes also showed positive binding, cerebellar granule neurons are the more promising binding source because of their high yield, good binding and biological relevance. in the second part, prp was constructed into a recombinant protein with human igg fc fragment ( prpfc ) and used directly to interact with its bindin
再次，在受體的鑒別和分離中，改進傳統的親和層析方法，以多功能交聯劑連接配體和受體，並分別以streptavidin及proteina偶聯的磁珠為結合反應載體，省lo以酉i
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