親和層析柱 的英文怎麼說

中文拼音 [qīnhécéngxīzhù]
親和層析柱 英文
affinity column
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 層 : i 量詞1 （用於重疊、積累的東西 如樓層、階層、地層） storey; tier; stratum 2 （用於可以分項分步的...
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 親和 : affine親和嫁接 congenial graft; 親和數 amicable number; friendly number
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation

    將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。
  3. The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1

    因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。
  4. But these techniques can not provide physiological ph and ionic strength, and radio - iodination of heparin required for ace endangers health of human and environment. for avoiding the radiological hazards and reflecting directly the binding characteristic of heparin to protein

    但親和層析柱易被污染失活， ace技術不能提供生理ph范圍和離子強度及ace技術必需的標記肝素的放射性物質對人和環境具有很大危害。
  5. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  6. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  7. After induced by 0. 1 m iptg, the pellet was resuspended in phosphate - buffered saline ( pbs ) / 5 mm edta, and sonicated three times for 20 s at maximal output

    上清液過blue一sepharose6b柱，進行一步親和層析，得到純化的rta和pj人~ kdel重組蛋白。
  8. With ni2 + - lda - sepharose, the cbd tag was removed. according to the results of sds - page and western blot analysis, the product of outflow was the target protein lt a 27. the purity of lt a 27 was about 95 %

    運用niz +金屬鰲合親和層析柱進一步快速純化去除融合頭，上樣流出經sds一隊ge分析和western印跡分析ii1 :明得到的純化產物為淋巴毒素缺失體蛋白lt凸27 ， lt 2 :產物純度可達95 %以上。
  9. The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively

    方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。
  10. The purity of expressed protein after treatment by gst affinity chromatography reached 90 % and at last the assay of inhibiting bacteria has been conducted

    表達的蛋白經親和柱層析，純度達90 ，最後進行了抑菌檢測。
  11. The expressed proteins were purified by ni2 + chelation affinity chromatography. the recombinant proteins were prepared with the purity more than 95 %

    用ni ~ nta親和層析柱進行純化，獲得1 』 rka膜外域各結構域重組蛋白，純度均在95 %以上。
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