親和純化 的英文怎麼說

中文拼音 [qīnhéchúnhuà]
親和純化 英文
affinity purification
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 親和 : affine親和嫁接 congenial graft; 親和數 amicable number; friendly number
  • 純化 : purification; purifying; depuration; edulcoration; purify
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  3. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  4. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation

    將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。
  5. The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1

    因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。
  6. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  7. The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab

    其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。
  8. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  9. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  10. Isolate and purify the target protein. prepare 50ml e. coli expressing the interesting proteins and harvest the cells and determinate the solubility of the target protein. completely solubilize inclusion bodies with 8m urea

    溶解包涵體，將包涵體溶解物上ni一taslurry ，利用該融合蛋白表達時帶有的his一tag與ni +的親和作用分離、純化表達蛋白。
  11. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  12. 2. the effect of ak auto ab on keratinocyte was investigated

    利用親和色譜法從正常人混合血清中純化了akautoah 。
  13. After induced by 0. 1 m iptg, the pellet was resuspended in phosphate - buffered saline ( pbs ) / 5 mm edta, and sonicated three times for 20 s at maximal output

    上清液過blue一sepharose6b柱，進行一步親和層析，得到純化的rta和pj人~ kdel重組蛋白。
  14. Gst - eorabl fusion protein and truncated gst - deeorabl fusion protein were purified by gst affinity chromatography and analyzed by cleaving with thrombin protease

    全長型融合蛋白經凝血酶酶切和進一步親和純化得到達電泳純的游仆蟲rab蛋白（ eorab1 ） 。
  15. Study on purification of rhepo by the immunoaffinity chromatography

    免疫親和層析法純化重組人促紅細胞生成素的實驗研究
  16. After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin, the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay. the assays demonstrated that the purified ntfs proteins exhibited normal activity, which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis

    最後，對重組表達蛋白經glutathlonesepharose4bmicrospincolumn親和純化后，進行大鼠腎上腺嗜鉻瘤細胞（ p2 ）神經營養因子的活性鑒定，發現其能夠誘導神經細胞分化產生突觸，即具有預期的生物學活性，表明所獲表達蛋白為大熊貓nt
  17. The percent of the fusion protein in that of the bacterium bodies is 23. 6 %, and the produced zz : g3 fusion protein present a single band after purified by affinity igg - sepharose. the systematic comparison and analysis of expression of pheromone 3 of euplotes in

    重組菌在0 . 4mmol / liptg誘導下進行分泌融合表達，融合蛋白的表達量約占菌體總蛋白的23 . 6 ，表達產物zz ： g3經igg - sepharose親和純化后基本為單一組分。
  18. The analysis of product solubility revealed that 5 - copy fusion protein mainly existed as inclusion body. the inclusion body was solulized in 8m urea and purified by poly - his protein purification kit

    將包涵體用8m的尿素溶解后，經poly - hisproteinpurificationkit親和純化，獲得純度為99的蛋白。
  19. Sds - page showed that the target proteins with approximate molecular weight, 31 kd could be detected in the lysates of the bacteria. ( 3 ) the rrta induced at 30 for 5h was soluble and fully active

    （ 4 ） 、通過誘導表達產生的蛋白質，經過blue一sepharose6b一步親和純化， sds一page檢查看出，只有一條分子量約為31000大小的條帶，表明純化完全。
  20. Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein. after stepwise washing and elution at purification procedure, sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6. 5 %

    用ni一nta樹脂親和純化表達產物，經過梯度漂洗和洗脫，獲得純度90 %以上的全長表達產物，另有6 . 5 %的非全長翻譯產物（約70kda ） 。
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