親和蛋白 的英文怎麼說

中文拼音 [qīnhédànbái]
親和蛋白 英文
rabphilin rab
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 親和 : affine親和嫁接 congenial graft; 親和數 amicable number; friendly number
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. This research reviewed the relationships of the myriapods to other arthropoda, based on morphological and molecular sequence data and the studies of developmental biology. the review was followed the major controversies in the higher - level phylogeny of myriapods. the complete 18s rrna sequences of four groups of myriapoda plus hexapoda ( protura, diplura, insecta ) and crustacea were used for phylogenetic study

    本研究首先從形態學性狀、分子性狀（ dna和蛋白質）以及結合形態和分子性狀、發育生物學的研究等方面對多足類動物與其他節肢動物之間的親緣關系進行了綜述，同時對多足類的系統發生研究進行了綜述。
  2. To his surprise, barrio found that fddnp had a specific affinity for the neurofibrillary tangles and beta - amyloid plaques found in the brains of alzheimer ' s patients

    令barrio吃驚的是，他發現fddnp對阿爾茨海默病患者腦中的神經纖維團和-澱粉樣蛋白斑有特別的親和性。
  3. On cytomembrane ; resolved the precipitation in buffer d ( contained triton x - 100 ) shattered by ultrasound, 4 vibrated for 60min, centrifuged at 45000g for 1 hour, took the supernatent to mesure protein concentration and then mesure the pkc activity

    目前，在鉛的神經毒性研究過程中，鉛對pkc的作用引起人們極大關注。這是因為鉛比鈣對pkc有更高的親和力。現已知， ph卜可以取代caz 」激活蛋白激酶c 。
  4. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  5. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  6. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  7. These changes were relevant to the concentration, the hydrophilic or hydrophobic nature and the length of arm spacers of crosslinker

    親水性edc對psll的蛋白質中tyr和trp殘基所處微環境的影響較小；而親脂性dcc 、 hmdi
  8. Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research

    傳統（如對復雜臨床樣品通過離子交換層析進行分離）和現代的方法（如通過親和捕獲與配體庫的組合富集低豐度蛋白）為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。
  9. Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

    通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1
  10. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation

    將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。
  11. The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1

    因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。
  12. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  13. The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab

    其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。
  14. It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating

    抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。
  15. The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before

    重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。
  16. Robinson p j, dunnill p, lilly d m. preparation of fine magnetic particles and application for enzyme immobilization. ibid, 1973 ( 15 ) : 603

    余藝華，薛博，孫彥，等.殼聚糖親和磁性毫微粒的制備及其對蛋白質的吸附性能研究.高分子學報， 2000 （ 3 ） : 340 344
  17. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  18. Isolate and purify the target protein. prepare 50ml e. coli expressing the interesting proteins and harvest the cells and determinate the solubility of the target protein. completely solubilize inclusion bodies with 8m urea

    溶解包涵體，將包涵體溶解物上ni一taslurry ，利用該融合蛋白表達時帶有的his一tag與ni +的親和作用分離、純化表達蛋白。
  19. A study on the regulating self - incompatibility of brassica oleracea l. by inhibitor or activitor of protein kinase

    利用蛋白激酶抑制劑和激活劑調控甘藍自交不親和性
  20. Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids

    本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析，同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析，以期為百合屬植物親緣關系分析提供生化依據，以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。
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