解離酶 的英文怎麼說

中文拼音 [jiělí]
解離酶 英文
lyase
  • 解 : 解動詞（解送） send under guard
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. Recently, the chinese plant protection agencies reported the growing hardships in controlling some field populations of oriental migratory locust with organophosphate ( op ) compounds. up to now there are more than 600 arthropod species with documented resistance to one or more insecticides and / or acaricides since resistance phenomenon was first described in san jose scale to lime - sulfur in washington in 1908. enhanced metabolic detoxification and reduced sensitivity of insecticide target - sites are the two major mechanisms in resistance development, involving three primary metabolic enzymes of esterases, glutathione s - transferases and cytochrome p450 polysubstrate monooxygenases

    代謝抗性是指對殺蟲劑起水解或隔離作用的酶發生改變，從而對殺蟲劑起到解毒作用，昆蟲主要解毒酶系有酯酶、谷胱甘肽- s -轉移酶、細胞色素p450單加氧酶等，這三種酶系任何一種的組成部分發生改變均會引起害蟲抗性的改變；靶標抗性是指由於殺蟲劑作用靶標敏感度降低而產生的抗性。
  2. Utilizing low denatured and defatted soybean flakers as material, a kind of functional food of soybean peptide nutrient solution which contains rich soybean peptide and little low - polysaccharide have been produced after the following production process : crushing, extracting, heat denaturing, enzyme digesting, acidifying, concocting, over - temperature sterling, canning, and so on

    摘要以低變性脫脂豆粕為原料，經粉碎、浸出、熱變性、酶解、酸化、分離、調配、超高溫滅菌、灌裝等工藝生產出一種富含大豆肽、少量低聚糖的大豆肽營養液功能性食品。
  3. The effort of manganese removal was studied and the kinetics of manganese removal was tried to establish. the factors of dissolved oxygen concentration, fe2 + concentration, ph, p concentration and closing of the filter were studied to evaluate their effort for biological manganese removal, and the correlation of residual manganese and oxidation - reduction potential was also discussed. as the iron content of water was high, experiment results showed that the reaction was zero order, as the iron content of water was low, the reaction was first order. the time needed for the cultivation of biological manganese removal was 60 70 days. the filter operated at the filtration rate of 8 10m / h, silica sand of effective size 0. 95 1. 25mm filled the filter to a depth of 1200mm

    試驗結果表明，成熟后濾砂表面濾膜的x射線衍射圖譜與mno _ x ? 5h _ 2o （ x = 1 . 86 ）的x射線衍射圖譜一樣，濾膜成熟后的結構在進水物質不發生變化的情況下不發生變化;合適的碳磷比對生物除錳有明顯的促進作用，試驗條件下的投磷量不會對出水造成二次污染;生物除錳需要亞鐵的參與，亞鐵的存在除了能夠促進微生物分泌胞外酶並刺激其活性外，還通過鐵離子的變價傳遞電子，催化錳離子的氧化反應，從而促進對二價錳的降解。
  4. For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development

    從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
  5. Here, we demonstrate on - line, real - time tryptic digestion in conjunction with reversed - phase protein separation

    這里，我們證明了與反相蛋白分離相結合的在線、實時胰蛋白酶解方法。
  6. Particularly advantageous appears the ability of the present method to fine - tune the separation of smallsize fragments and tryptic digests, where conventional sds - page usually fails

    該方法在傳統sds - page通常不適用的小的碎片和胰酶解物的分離表現出特別的優勢。
  7. Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion

    差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。
  8. 1. a new method to identify _ amylase activity and its producing bacteria : the blue complex was formed by unspecific adsorption, after mixing starch and trypan _ blue. the adsorption weakened when the starch was hydrolyzed to small molecular by _ amylase, and the trypan _ blue was released inside the hydrolyed zone. the starch around the zone which was not hydrolyzed adsorbed free trypan - blue so that the colour of medium became bluer than that of place in hydrolyzed zone

    快速鑒定並篩選-澱粉酶及其產生菌的新方法：錐蟲藍染料和澱粉由於靜電非特異性吸附結合后使澱粉呈穩定的藍色，當澱粉被澱粉酶水解后因分子變小吸附力減弱，而讓錐蟲藍游離出來，游離的錐蟲藍被周圍未水解的澱粉吸附而使顏色加深，澱粉水解區則形成無色、透明的水解圈。
  9. Elevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus

    這樣，可能使得萎縮比目魚肌細胞內鈣離子水平升高，細胞內鈣離子靜息濃度的增加一方面激活calpain ，增加收縮蛋白的降解，使肌肉萎縮；草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶，增加快型mhc基因的表達，使骨骼肌肌球蛋白重鏈（ mhc ）發生由慢型向快型的轉化。
  10. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  11. The paper deals with the isolation and identification of a creatinase - producing bacterium from soil, the conditions for production of creatinase, purification methods of creatinase and the purified enzyme characteristics

    1 、產肌酸水解酶微生物的分離及鑒定本研究用恆化培養的方法從土壤中富集微生物，然後再用平板劃線分離。
  12. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
  13. According to the comparation among the three kinds of crude extraction methods including acid dissolve, enzymolysis and neutral salt solution. the method of enzymolysis combined with hplc was chosen to prepare cp i. the physico - chemical property of, cp i was identified

    以豬皮為材料，比較酸溶法、酶解法、中性鹽溶液法三種粗提方法，選擇得率和純度都較高的酶解法為最佳粗提法，結合高效液相色譜進行cp的分離純化。
  14. There are six systems of biohydrogen production which include biophotolysis, organic degradation of light, hydrogen synthesis via the water - gas shift reaction of photoheterotrophic bacteria, hybrid system of photo - fermentation, anaerobic fermentation and in vitro hydrogen production by hydrogenase

    討論了光合成生物制氫系統、光分解生物制氫系統、水氣交換反應生物制氫系統、光合發酵雜交生物制氫系統和厭氧發酵生物制氫系統、離體氫酶生物制氮系統等6個生物制氫系統。
  15. Moreover, the mass spectrum analysis of the hydrolyzed water soluble products of phb powder after treatment of enzyme was performed and the main product was identified to be dimers

    一些金屬離子對phb解聚酶有抑制作用。質譜儀測得酶降解phb的產物主要是二聚體。
  16. Conclusion the selective dissociating effect of the detergent on hdl is extremly important and helps the modified enzymes to selectively reacts to hdl - c

    結論：表面活性劑對高密度脂蛋白的選擇性解離作用非常重要，這有助於修飾酶發揮其對高密度脂蛋白膽固醇的反應選擇性。
  17. A single molecule of rta is able to depurinate 1500 - 2000 ribosomes per minute. ricin b chain ( rtb ) is a galactose - specific lectin which binds to the receptors on cell surfaces, and may enhance rta translocation by forming a pore in the membrane of intracellular organelles. ricin enters the cells by receptor - mediated endocytosis

    內吞進入細胞的蓖麻毒素一部分返回到膜表面，一部分經早期內體至晚期內體，最後在溶酶體中降解，只有一小部分約5到達高爾基體，隨后逆轉運到粗面內質網，在那裡rta才和rtb解離，游離出有催化活性的rta 。
  18. Subsequently, the binding of ( 35 ) ~ s - cam and a. thaliana protoplasts were processed, including association, dissociation, competitive and saturation analysis. the results show the binding of ( 35 ) ~ s - cam and protoplasts is rapid, reversible, saturable and sensitive to proteinase ; otherwise the specificity of binding was assessed with different competitors. k _ ( d ) of 9. 2 nm and about 25, 000 binding sites per protoplast were gained through saturation analysis

    隨后，對~ （ 35 ） s - cam和擬南芥原生質體的結合進行了受體學分析，包括結合、解離、競爭性和飽和分析，結果顯示~ （ 35 ） s - cam和擬南芥原生質體的結合具有結合快速性、可逆性、競爭特異性和濃度飽和性，從飽和分析可以得出k _ d約為9 . 2nm ，每個原生質體表面大約含有25 ， 000個結合位點，並且其結合對蛋白酶敏感。
  19. By this means, modifying the tnpi gene can get the different vectors with different resolution speed. 3

    若在解離載體中引入整合酶基因，在大腸桿菌中無法篩選到轉化子。
  20. Effect of integratase tnpi on resolution reaction of resolution vector the integratase tnpi has great effect on the speed and time of resolution reaction

    整合酶對載體解離特性的影響整合酶對解離載體的解離速率和時間影響很大。
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