誘變性檢測 的英文怎麼說

中文拼音 [yòubiànxìngjiǎncè]
誘變性檢測 英文
mutagenicity assay
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 檢 : Ⅰ動詞1 （查） check up; inspect; examine 2 （約束; 檢點） restrain oneself; be careful in one s c...
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  • 檢測 : check; detection; test; gauging; detecting; sensing; [工業] checkout; measuring
  1. Methods to be applied include extracellular ca2 + influx treatment and intracellular ca2 + measure by transgenical expression of ca2 + fluorescence indicator during fertilization. the former is first to obtain protoplasts of female germination unit in different development stages, then ca2 + lonophore treatment ; the latter is to express transgenically the ca2 + - sensing cameleon protein in torenia fournieri, which allows long term measuring stimulus - induced cytoplasmic ca2 + changes

    工作之一首先是檢測受精過程中雌性生殖單位中發生的顯著的細胞學變化，以此作為受精與否的標志；然後經過外源導入鈣（ a23187誘導）研究[ ca ~ （ 2 + ） ] _ （ cyt ）在受精過程中的這一細胞學事件發生中所起的作用。
  2. In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure

    我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。
  3. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  4. Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells, ros and the membrane potential of mitochondria during this process were measured. our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells, led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential, and caused senescence of 2bs cells rapidly. it supports the hypothesis of oxidative damage of senescence. moreover it is a better system for the study of the effects of ros during the process of replicative senescence

    利用光學顯微鏡觀察和酸性-半乳糖苷酶染色技術研究了高濃度葡萄糖對人二倍體成纖維細胞2bs細胞衰老進程的影響，並用流式細胞儀檢測了此過程中活性氧和線粒體膜電位差的變化。結果表明： 200 mmol l的葡萄糖對2bs細胞有生長抑制作用，能引起活性氧含量的變化，導致線粒體膜電位差顯著下降，並誘導了細胞的衰老。這為氧化損傷假說提供了新的證據，並為研究活性氧和復制衰老之間的關系提供了較好的體系。
  5. Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector

    為了檢測得到的啟動子驅動效率及誘導活性，將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接，構建了6個植物表達載體，同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。
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