豬苗代 的英文怎麼說
中文拼音 [zhūmiáodài]
豬苗代
英文
inawashiro-
On the basis of the separation and identification of streptococcosis suis in the southern part of henan province, the certain typical bacterial strains were selected as the vaccine strains to produce polyvalent inactivity vaccine for the local prevention of the disease
摘要在對豫南地區豬鏈球菌病病原分群鑒定的基礎上，挑選具有一定代表性的菌株作為疫苗株，研製多價滅活疫苗免疫防制豬鏈球菌病。Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified
為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。
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