貝隆多 的英文怎麼說
中文拼音 [bèilōngduō]
貝隆多
英文
belondo-
" breast ironing is an age - old practice in cameroon, as well as in many other countries in west and central africa, including chad, togo, benin, guinea - conakry, just to name a few, " said flavien ndonko, an anthropologist and local representative of german development agency gtz, which sponsored the survey
一位人類學家及贊助這項調查的德國當地發展局代表福雷門說:燙胸部在柯麥隆是項流傳已久的習俗,這項習俗同時也在許多西方國家跟中非,包含查德、多哥、貝南、幾內亞首都科那克里,都記載不多。There are more than 400 diploma mills and 300 counterfeit diploma web sites, and business is thriving amid a lackluster 10 economy ? doubling in the past five years to more than $ 500 million annually, according to estimates kept by john bear author of bears ' guide to earning degrees by distance learning
據《貝爾指南? ?如何通過遠程教育而獲得學位》的作者約朝翰?貝爾估計,美國現有400多家「文憑作坊」和300多家假文憑網站;經濟衰退期間,這一行可謂生意興隆? ?過去5年裡,其年收入翻了一番,達5億多美元。With master s blessing, during these five years, brother chine and local fellow initiates have spread master s teachings in togo and the neighboring countries of benin, cameroon, ghana and burkina faso, and more truth - sharing activities are planned for the future
在這段期間內,由於師父的加持,簡師兄和當地一些同修得以將師父的教理傳播到鄰近其他國家,包括貝南科麥隆迦納布吉納法索等,他們並計劃未來將繼續擴展到更多國家。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page電泳分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的條帶。This past week i was asked to participate at remembrance service for dr. arnold beckman, one of the greatest men ever to live in orange county, california
上周我出席了一個隆重的喪禮,不是列根的喪禮,而是貝克曼的追思禮拜。多少人聽過貝克曼這名字?In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。At yesterday ' s presentation for the capture of midfielder valon behrami, lotito declared : " we have strengthened considerably the midfield unit with the insertion of some great figures and equally this will happen in defence
在昨天發表的對于搶到中場球員瓦隆?貝赫拉米,羅蒂托說道: 「我們已經得到了加強,相當多的好球員加入到中場,同樣的(事情)也會發生在後防線上。 」Real president ramon calderon said many times in the autumn that he wanted to extend beckham s contract
皇馬俱樂部總裁拉蒙卡爾德隆說,今年秋天他多次想與貝克漢姆續約。Real president ramon calderon said many times in the autumn that he wanted to extend beckham ' s contract
皇馬俱樂部總裁拉蒙卡爾德隆說,今年秋天他多次想與貝克漢姆續約。In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列,同時考慮構建和表達的需要,化學合成了misgurin基因和接頭,採用一種新的策略,在體外將基因多拷貝同向串連,並將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Good move by inter. veron passes to cruz, whose first - time pass sets up adriano. oddo does well to get back and regain possession
精妙地配合!貝隆傳給克魯茲,克魯茲第一時間分球給阿德,但奧多做出了一次很好的攔截。Sequencing requires multiplecloned copies of the gene ; long dna sequences are cut into more manageable lengths using restriction enzymes
序列需要基因的多個克隆拷貝,用限制性酶可以把長的dna序列切成許多具所需長度的片段。At eight oclock kutuzov rode out to pratzen at the head of miloradovitchs fourth column, the one which was to occupy the place left vacant by the columns of przhebyshevsky and langeron, who had by this time gone down to the plain
八點鐘,庫圖佐夫騎馬前赴米洛拉多維奇的第四縱隊前面的普拉茨村,第四縱隊必須接替已經下山的普熱貝舍夫斯基縱隊和朗熱隆縱隊。分享友人