質體基因 的英文怎麼說
中文拼音 [zhítǐjīyīn]
質體基因
英文
plastogene-
The development of a multicellular organism results from the selective expression of genes and the maturation of proteins is dependent on molecular chaperons
多細胞生物體發育的實質是基因的選擇表達性,表達蛋白的成熟則有賴于分子伴侶的協助。Etioplasts have the capacity to synthesize plastome-coded proteins.
黃化質體也有能力合成質體基因組所編碼的蛋白質。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。The retention tune of each of the three main peaks of the antibiotic fr - 008 is entirety identical to candicidin, except for one peak ( fr - 008d ), which can also be seen as a very weak signal on the hplc profile of candicidin with the same rt
因此可以推測,這兩個菌株在表型上的差別有可能與鏈黴菌fr - 008中的線性質粒有直接的關系,但也不能排除染色體基因之間的差異。Molecular systematics of partial species belonging to 5 subfamilies of miridae are investigated in this study by using esterase isozyme ( est ) and cytochrome b ( cyt b ) gene in mitochondrial dna as molecular marks
運用酯酶同工酶和線粒體dna中編碼細胞色素b蛋白質的基因cytb作為分子標記,對盲蝽科miridae的5個亞科的部分種類進行了分子系統學研究。Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas. its latex is the unique source of crude rubber used in current industry. because of its special and important use, the rubber tree has been extensively planted in tropical areas. increase production is always the main target in rubber tree cultivation. since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant, not only the latex production had been increased largely, but also a new set of rubber tapping system had been established, leading to a series of economic benefit. owing to ethrel " s extensive application, its side effects had been found more and more obviously, such as tapping dry, speeding up senescence, shortening the life span of rubber tree etc. in order to overcome the side effects and increase production more availably, for a long time, people had carried out lots of research work on cell level, membrane level, physiology and biochemistry of laticifer contents. but the mech anism why ethrel increased latex production was not yet understood completely. this study had cloned the ethylene receptor gene ( efrl ) from rubber tree, and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level. the results were as follows : 1
但是,由於乙烯利應用的普及,乙烯利刺激割膠引起橡膠樹發生死皮病及加速膠樹衰老,縮短膠樹壽命等副作用也越來越明顯。為了克服這些副作用,使乙烯利能更有效地刺激增產,長期以來,人們在細胞水平、膜水平和乳管細胞內含物的生理生化層面上進行了大量的研究,但仍未完全了解乙烯對膠樹的作用機制。本研究從分子水平入手,克隆橡膠樹的乙烯受體基因( etr1 ) ,並在轉錄水平和蛋白質翻譯水平上研究etr1基因在乳管細胞中的表達及與乙烯刺激的關系,取得了以下結果: 1Therefore, for cardiomyopathies in children, after exclusion of non - genetic causes, genetic studies and metabolic screening, such as tandem mass spectrometry, urine gas chromatography mass spectrometry, carnitine level and mitochondrial dna screening, are recommended
因此針對一個兒童時期心肌病變的病人,在排除了非遺傳因素造成的原因后,應當進行遺傳與代謝疾病的篩檢:包括血液串聯質譜儀檢查、尿液有機酸檢查、血液肉堿濃度檢測與粒線體基因篩檢等。The technology and methods of the study on molecular mechanism of cytoplasm male sterility ( cms ) are introduced in regard to mitochondrial genome, mitochondrial gene, mitochondrial rna, mitochondrial protein, transformed plants and abortion of pollen
摘要本文從線粒體基因組、線粒體基因、線粒體轉錄rna 、線粒體蛋白、轉基因植物以及花粉敗育機理六個方面詳細介紹了植物細胞質雄性不育分子生物學研究的技術和方法。A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段,將此基因片段克隆至表達載體pet36b ( + )上,與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 , pcr擴增其scfv基因,篩選重組子進行序列測定,發現其序列符合小鼠抗體基因的一般特徵,並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上;推測pcsa1scfv針對的抗原是磷酸膽堿類物質。All eukaryotes ( organisms with nuclei in their cells ) use a molecular translator enzyme called rna polymerase ii to read the genes that are expressed into proteins
所有的真核生物(細胞里有核的生物體)利用一種稱為rna聚合酶ii的分子轉錄酵素,來讀取蛋白質的基因。Because there are many copies of chloroplast dna and the chloroplast has a strong tolerance to accumulation of the products expressed by the introduced foreign gene, a high level of expression is often happened in chloroplast transformation. in addition, because of prokaryotic property of the chloroplast, the prokaryotic gene can be expressed in chloroplast without any modification and multigene can be simultaneously transferred in " polycistron ", which is impossible in nucleic transformation
另外,由於葉綠體基因組的原核性質,對來自原核生物的外源基因無需改造就可以在葉綠體內高效表達,而且可以將多個外源基因採取「多順反子」的原核表達形式同時引入,並由共同的啟動子控制,既方便操作又可避免由於存在多個相同啟動子所帶來的「共沉默」 。Quinolones, tetracyclines, aminoglycosides, and so on. antimicrobial peptides are a new class of antibiotics discovered recent years, which are encoded by genes of organisms and chemically peptides, so they are also called as peptide antibiotics
肽抗生素( peptideantibiotics )或稱為抗微生物肽( antimicrobialpeptide )則是近年來發現的由動物、植物、昆蟲等生物體基因編碼的具有抗生素樣活性的肽類物質。It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0. 5 - 3kb fragments of a. brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors
Brasilensesp7的nifa全序列構建在pgbd - c2載體上,得到重組質粒pgbd - nifa ,以其為誘餌,篩選sp7質粒基因組文庫(構建在pgad系列質粒上) 。Effects of nmda receptor on glucocorticoid receptor mrna levels in hippocampus of rats after scald burn stress
受體對燙傷應激大鼠海馬糖皮質激素受體基因表達的影響It encodes a protein of 865 amino acids with a signal peptide at the n - terminus, of which a polycystic - kidney - disease ( pkd ) - like domain, a triosephosphate - isomerase ( tim ) catalytic region, a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus
根據已知幾丁質酶基因的dna保守序列,利用人工合成的pcr引物和cb101總dna ,擴增出一個約2 . 6kb的片段並克隆到pbluescript ks載體上。By contrast, l - methionine increased the level of methylation and thus reduced the expression of the gene in animals with loving mothers, and led to fear, anxiety and a heightened response to stress
與之相反, l -甲硫氨酸增加了甲基化水平,從而減弱了正常老鼠中糖皮質激素受體基因的表達,導致了恐懼、焦慮和對壓力的強烈反應。Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained
用ecori和sall對含有幾丁質酶基因的重組質粒tvchi (含幾丁質酶編碼基因的pmd18一t載體)和穿梭質粒phy300plk進行雙酶切並連接,轉化e . colidhs 。Sds - page and activity staining analysis revealed that all of clones 2, 5 and 9 exhibited a single active band with 83 - kd in size, whereas clone 3 exhibited four different bands with 64 kd, 70kd, 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5, a complete nucleotide sequences comprising of 3, 205 bps wes determined. one open reading frame ( orf ) was found in this sequence, which was comprised of 2, 661 bps and could encode a polypeptide with 887 amino acid residues
利用基因分析軟體genetyx對clone - 5質粒的核苷酸序列進行分析,並搜索日本dna數據庫( dnadatabankofjapan ,簡稱ddbj ) ,推斷clone - 5質粒的幾丁質酶基因含有一個2 , 261bp的開放閱讀框( orf ) ,編碼一條887個氨基酸的幾丁質酶蛋白; orf上游有一個啟動子。It is used to probe the relationship between the structure and activity of proteins ; to define the roles of particular proteins and protein assembles in the cell ; to determine the logic and order of molecular events during differentiation and morphogenesis ; to improve the activity of enzyme, antibiotics and so on
引導蛋白質功能進化常用的方法是模擬和加速蛋白質基因自然重組的進程,即在蛋白質的基因中引進隨機突變。因此,蛋白質基因體外隨機突變的方法影響著引導蛋白質功能進化的效果。分享友人