赤木達 的英文怎麼說
中文拼音 [chìmùdá]
赤木達
英文
chimda-
The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Again, i do not recommend that you take the risk to get to the scene. make do with the annual report and enjoy a safe hike to ham tin, where you should get off the trail, go on to the beach, cross the shaky bridge at the end, have a good lunch and refill at the restaurant run by my old friend mr lai
我們繼續走到咸田，然後離開麥理浩徑，到達沙灘，經過沙灘盡頭那道搖搖晃晃的獨木橋，在我的老友黎先生的士多吃頓午餐，休息一下，然後再踏上士多後面的路回到麥理浩徑上，一直前往大浪坳赤徑及北潭凹。The diameter is 11. 5 centimetres, whole wooden tire, red treasure sand bottom, adopt gold and silver to be flat to take off skill and technique, is it make various kinds of flowers line kinds to carve gold and silver thin slice, paste in painting foetus smooth of polishing with paint, after being dry, overall xiu several of celluloid paint is it show the honeysuckle line to grind and then, feel abundant, pattern and paint bottom reach same pingdu, push away light become a exquisite one flat to take off the lacquer ware in addition
直徑11 . 5厘米，整木車胎，赤寶沙底，採用金銀平脫技法，將金銀薄片刻製成各種花卉紋樣，用漆粘貼在打磨光滑的漆胎上，待乾燥后，全面髹透明漆數層再經研磨顯出金銀花紋，質感豐富，花紋與漆底達到同樣平度，再加推光則成為精美的平脫漆器。The classic blend of cabernet with merlot is balanced wonderfully with a hint of oak designed to enhance the fruit
混合赤霞珠和梅洛的經典之釀，達到了完美的平衡口感，橡木桶的芳香增強了果香的口感。Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a
我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。分享友人