載體上的生物 的英文怎麼說

中文拼音 [zǎitǐshàngdeshēngwù]
載體上的生物 英文
biology on support
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 上 : 上名詞[語言學] （指上聲） falling-rising tone
  • 的 : 4次方是 The fourth power of 2 is direction
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  • 生物 : living things; living beings; organisms; bios （pl bioi bioses）; biont; thing; life生物材料 biol...
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. The stresses acting over the surface of the body must produce tractions which are equivalent to the loads being applied to the body.

    作用在物體整個表面上的應力所產生的面力，必須與作用在物體上的載荷相等。
  3. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  4. The nanoporous silicon membrane can be used as drug carrier, immuniosolating biocapsules, nanoporous silicon micromirror and biosensors in medical field

    在醫學上的納米多孔硅膜可作為藥物載體、免疫隔離生物膠囊、納米硅微鏡和納米多孔硅生物傳感器。
  5. The chromosome is the carrier of genetic material. there are genes on it. it decides human being ’ s configuration and physiological function

    染色體是遺傳物質的載體，它上面帶有遺傳因子，決定人體的形態特徵和生理機能。
  6. ( 6 ) conclusion was obtained by the analyzing the mechanics environment and the stability of the lanslide on the right bank after the dam built : the value of the rock mass stress increased and the tensile stress region obviously reduced, the direction of the principal stress was unchangeable the dam and the landslide were stable under the dangerous condition ( the reservoir and at the same time the earthquake was viii ), but when the water lever suddenly fell and did not think of other force, safe factor evidently reduced, the dam and the landslide will be destroyed under the most dangerous condition ( the water lever suddenly fell and at the same time the earthquake was vi ) ; the stability of the landslide will be destroyed by the horizontal thrust under the most dangerous condition or ; the physical and mechanical parameter will be reduced due to long period filter, the landslide will be destroyed too

    （ 6 ）建壩工程荷載條件下河谷巖體力學環境分析及右岸古滑坡體的穩定問題分析得出：建壩后河谷的巖體應力量值明顯增高，同時左岸的張應力區的范圍及量值明顯減小，主應力的方向依然與模型的底邊界垂直；大壩建成后逐漸蓄水階段以及同時考慮本區最強地震力的作用等各種不同的工況條件時，滑坡體及壩體總體上仍處于穩定狀態；水位驟降時，在未考慮其他外力作用的情況時，滑體的安全系數將顯著降低。若考慮水位驟降及本區最大地震力的共同作用，壩體將在滑坡體下滑推力的作用下產生破壞；建壩后水平推力對右岸古滑坡體穩定性的影響分析可以看出，在最危險的工況條件下，壩體及滑坡體也將處于臨界狀態；在長期滲透變形的作用下，由於滑帶的物理力學參數的降低而有可能導致壩體失穩破壞。
  7. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  8. Starch can not be digested in the upper gastrointestinal but can be microbially decomposed in colon by suitable modifications which control starch digestibility, thus it can be used as carrier for oral colon - specific drug delivery system

    通過合理改性來調控澱粉的消化性能，使其具有不被上消化道消化、但能夠被結腸微生物酵解的特性，則可用作口服結腸靶向藥物控釋載體。
  9. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  10. As the carrier of knowledge and enterprise production element, human capital is the capital which is cohered to labor, transformed through capital invested fees, and is the representation of the labor skills and technique. or human capital present in the labor and it is the nonmaterial capital which is expressed through the quality and quantity of the labor. so the human capital becomes the key of the enterprise to exist and develop

    而人力資本作為知識載體和企業生產要素，是指凝結在勞動者身上，由資本投資費用轉化而來的，表現為勞動者技能和技巧的資本，是一種體現在勞動者身上，以勞動者的質量和數量所表示的非物質資本，也因此而成為企業賴以生存和發展的關鍵。
  11. The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination

    為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。
  12. The sol - gel film showed the excellent chemical and electrochemical properties as well as kept the quality electrochemical activity of those immobilized redox molecules. the modified molecules took place the typical adsorption controlled electrochemical reaction, and furthermore, the modified methylene blue gave the obviously electrochemical catalytic affection on oxidation of nadh or reduction of hemoglobin if they were immobilized in the film meanwhile

    同時以硅溶膠?凝膠膜為載體制得化學修飾電極，用sol - gel法在金電極上固定亞甲藍、硫堇及茜素s ，發現固定於納米溶膠?凝膠膜內的亞甲藍和硫堇均有良好的電化學活性，對同時固定於膜內的nadh 、血紅蛋白等生物分子產生顯著的催化氧化還原作用。
  13. Pcr products were inserted into pbv - 220 with double digestion of restriction enduonuclease. the expression vectors of pbv - a pbv - b and pbv - c were constructed by orientaional cloning. through sds - page, bioactivity and function analysis of expressed protein, the function of phaa, phab and phac was confirmed

    菌株的亞克隆基因組片段中，分離出phaa 、 phab和phac三個基因片段，定向克隆至原核表達載體pbv220上，構建了三個原核生物表達載體pbv - a 、 pbv - b和pbv - c ，通過對表達載體誘導表達，表達蛋白產物的sds - page分析、生物活性與功能分析，確定了基因phaa 、 phab和phac的生物學功能。
  14. A genomic dna library of atriplex centralasiatica iljin was constructed using lambda embl3 / bamh i as vector

    為了得到badh基因的上游序列，我們以lambdaembl3 budl為載體，構建了鹽生植物中亞濱茵的基因組文庫。
  15. Storing information in molecules of dna could allow for an information density of approximately 1 bit per cubic nanometer. the energy consumed by dna molecular biology computing is billionth of that consumed by one conventional computer. the characteristics of dna molecular biology computing mentioned above which are high parallelism, huge capability and low consumption are incomparable and irreplaceable to the existing computers and parallel ones

    Dna分子生物演算法具有高度的并行性，運算速度快； dna作為信息的載體其貯存的容量非常之大， 1立方米的dna溶液可存儲1萬億億的二進制數據； dna分子生物計算所消耗的能量只有一臺電子計算機完成同樣計算所消耗的能量的十億分之一； dan計算的上述特性，即運算的高度并行性、大容量、低消耗是目前計算機和并行計算機所無法比擬和替代的。
  16. Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage

    構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
  17. Two primers were desighed and synthesized. xynbj coding nature protein of xynb have been cloned by pcr. then xynd1 - s nyna ] - w and xynb1 were all cloned into the plasmid vector pet - 22b ( + ), and were all expressed in e coli

    將此序列克隆進表達載體pet - 22b （ + ）上，在大腸桿菌bl21 （ de3 ）中也得到特異性表達，並有正常的生物學活性，證明了基因xynb1的生物學功能。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  19. A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body

    本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。
  20. Based on the plant expression vector of pcambia1300 - hbmp - 3m and pcambial 301 - hbmp - 3 constructed, we set up the system of high frequency regeneration and transformation of tomato cotyledons. the hbmp - 3m gene and hbmp - 3 gene were respectively transformed into tomato and tobacco by transfection of agrobacterium tumefaciens. and the transgentic plants were detected. the main results are as follows : l. the plasmid pcambial300 - hbmp - 3m carried hbmp - 3m gene and pcambia - 1301 - hbmp - 3 carried hbmp - 3 gene were respectively transferred into agrobacterium tumefaciens by electroporation

    本試驗是在已經構建好的植物表達載體pcambia1300 - hbmp - 3m和pcambia1301 - hbmp - 3載體的基礎上，建立番茄子葉的高頻率再生體系和番茄子葉的高頻遺傳轉化系統，利用根癌農桿菌介導的方法，將人骨形成蛋白- 3成熟肽（ hbmp - 3m ）基因和人骨形成蛋白- 3 （ hbmp - 3 ）全長基因分別導入番茄和煙草，並對轉基因植株進行了檢測。
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