載體宿主體系 的英文怎麼說

中文拼音 [zǎitǐxiǔzhǔtǐxì]
載體宿主體系 英文
vector-host system
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 宿 : 宿名詞[天文學] （星的位次） an ancient term for constellation
  • 系 : 系動詞（打結; 扣） tie; fasten; do up; button up
  • 載體 : [化學] carrier; supporter; isotopic carrier
  • 宿主 : [生物學] host; parasitifer
  1. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  2. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  3. Different expression systems were constructed using different expression vector including pet - his and e. coli topi of " and bl21 host cells

    利用pet - his等不同表達載體和大腸桿菌top10f 、 bl21 （ de3 ） plyss表達宿主菌，成功構建了膽固醇氧化酶基因的不同原核表達系統。
  4. This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi - copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure, etc

    摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。
  5. The expression mixture were eletrophoresized by sds - page, revealing a specific 56 kd protein in bl21 expression system, which is of the same size in expectation. a colorimetric assay for measuring cholesterol oxidase showed the cholesterol oxidase activity of the expression product

    其結果經sds ? page分析表明：以pet - his為表達載體，大腸桿菌bl21 （ de3 ） plyss為宿主菌的重組表達系統表達出約56kd的特異蛋白，蛋白大小與預期結果相一致。
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