載體抗體 的英文怎麼說

中文拼音 [zǎitǐkàngtǐ]
載體抗體 英文
carrier antibody
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. This study adopted the ion compound antibacterial to produce the materials of antibacterial glass. two kinds of different carriers are used in this experiment, phosphate and borate system. the antibacterial glass material, which is added ag +, zn2 + through some carriers, has excellent antibacterial property against escherichia coli and staphylococcus aurous

    實驗中採用兩種不同的玻璃載體體系，即磷酸鹽載體和硼硅酸鹽載體，將銀、鋅離子以一定的方式直接加入到玻璃生產的配合料中，一次性熔製成形，能夠制備出對大腸桿菌、金黃色葡萄球菌等細菌具有良好抗菌效果的抗菌玻璃材料。
  3. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  4. In recent five years, some important proceedings have been carried out, including being used as a new nutrition agent or protective carrier for new sensitive components, an enzyme micro - capsulated reactor, a control - released carrier of special food components, an assessment system for anti - oxidation of food component, a tool for food analysis and development of new liposome with natural and safe characteristics

    近5年來，脂質體在充當營養新劑型或敏感成分保護性載體，構建食品酶微囊反應器，作為特殊食品組分的緩釋載體，充當食品組分抗氧化評估體系，充當食品分析工具以及天然、安全的新穎食品脂質體構建等方面的研究與應用，均取得了一些重要進展。
  5. The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated

    Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。
  6. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  7. Expression vectorion construction of hepatitis b surface antigen gene and transformation to crowtoe lotus corniculatus

    乙肝表面抗原基因表達載體的構建及對百脈根的轉化
  8. Study on diatomite as antiseptic agent carrier

    硅藻土作為抗菌劑載體的研究
  9. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  10. The protein accounting for the total was approximately 11. 80 %. the peptide was synthesized according to the sequence of grb - ast7. since ast7 is a hapten, it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc )

    根據ast _ 7氨基酸序列合成小肽，用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa測定效價為1 400 。
  11. Results indicate that lactobacillus casei possesses probiotic properties and can be used as oral vaccine carrier to deliver heterologous antigen

    結果表明：乾酪乳桿菌符合益生菌的標準，可作為遞呈疫苗抗原活菌載體。
  12. As a rule, the immunosensors is to coat the surface of crystal with antibodies or antigens, immerge to muster solution, binding to the immobilized antigens or antibodies and take place immuno - reaction, can cause a frequency change of the quartz crystal, and the frequency changes are proportional to content of antigens or antibodies in muster solution

    通常將抗體（或抗原）固定於晶體表面，浸入樣液時，與抗原（或抗體）產生免疫反應，使晶體表面質量負載增加，頻率降低，其頻移值與樣液中抗原（或抗體）含量成正比。
  13. The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwich or competitive immunoreaction

    將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。
  14. The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwitch or competitive immunoreaction. the surface of immuno - sensor can be renewed by used in a new immunoassay

    將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。
  15. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  16. In addition, phenylalanine is used in aspartame production and acts as intermediate and well vector in synthesis of some anti - cancer drugs

    苯丙氨酸既是合成新型甜味劑aspartame的原料，又是合成一些抗癌藥物的中間體和良好載體，市場需求日益增加。
  17. Influence of properties of chitosan nanocarrier material on immune activity of anti - hepatitis b immune ribonucleic acid

    殼聚糖納米載體材料性能對抗乙肝免疫核糖核酸免疫活性的影響
  18. The native expressed product from e. coli bl21 ( de3 ) strain, however, showed weak activity against tmv. gp609 and zwemu were inserted into pemu - mcs - n, an expression vector for monocotyledon. and rhxjb was inserted into pkylx71 : 35s2

    將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi （ de3 ）菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。
  19. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  20. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
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