轉化蛋白 的英文怎麼說
中文拼音 [zhuǎnhuàdànbái]
轉化蛋白
英文
transforming protein-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。At rest, the heterodimeric rel / nf - k b complex is located in the cytoplasm bound to an inhibitory factor, i k b. upon stimulation, i k b is phosphorylated and degraded, free nf - k b then translocates into the nucleus where it binds to k b site to regulate transcription
靜息時， re皿4bh聚體與抑制蛋白kbs結合，在細胞漿中保持無活性。受到刺激， ikb分子迅速磷酸化並降解，釋放nf兒b轉位入核，通過與kb位點結合調節轉錄。The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed
用活體核磁共振定域氫譜（ in - vivohmagneticresonancespectroscopy ， in - vivohmrs ）對胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析；用活體磷譜（ in - vivo 』 』 pmrs ）的方法分析了在整個胚胎發育過程中含磷代謝物的相對含量隨時間的變化，表明了磷脂類物質及三磷酸腺苷（ atp ） 、磷酸肌酸（ pcr ）在此過程中的變化及可能的相互轉化的趨勢，胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸腺苷（ atp ） 、磷酸肌酸（ pcr ）在死亡過程中降解為無機磷的現象。Thrombin is an enzyme that catalyzes the conversion of the soluble plasma protein fibrinogen to the insoluble protein fibrin.
凝血酶能催化可溶性血漿纖維蛋白轉化為不溶性的纖維蛋白。In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein
在胃裡，這種酸的作用是殺司食品的細菌，使食物軟化，把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。By observing the effect of acidity on the uv difference spectra of ehpg or hbed, the spectral characteristics of apoovotransferrin binding to metals and anions at ph 7. 4 show that the reaction of apoovotransferrin with metals is related to deprotonation of the phenolic group of tyrosine of the metal binding sites, while the binding of apoovotransferrin to anions is associated with the protonation of the phenolic group of tyrosine of the anion binding sites
摘要通過觀察酸度對模擬轉鐵蛋白小分子ehpg和hbed紫外差光譜的影響，結合脫鐵伴清蛋白與金屬離子、陰離子結合的光譜特性，推斷脫鐵伴清蛋白與金屬離子的結合導致金屬離子結合位點處酪氨酸酚羥基的去質子化，而與陰離子的結合導致陰離子結合位點處酪氨酸酚羥基的質子化。Change of serum transferrin in children with positive hemoculture
血培養陽性敗血癥患兒血清轉鐵蛋白變化The first two methods are the most commonly used for soybean transformation. the other methods have not been optimized for soybean, and are therefore less efficient and not often used. previously, we isolated ssnhxl ( encoding a vacuolar na + / h + antiporter ) from s. salsa, and further research was done through the overexpression in arabidopsis thaliana, it could help us to elucidate the salt tolerance of halophyte
本實驗室成功的從鹽地堿蓬中克隆到位於液泡膜上的ndm 」反向轉運蛋白基因ssnhx ，轉化擬南芥並對該基因的功能進行了分析，發現在擬南芥中過量表兇亥基因可以巳著提；削6物的燦處性。Renin, a proteolytic enzyme formed in the granules of the juxtaglomerular apparatus cells, catalyzes conversion of the protein angiotensinogen to angiotensin i, a decapeptide
腎素是腎小球旁體細胞顆粒內形成的一種蛋白水解酶，催化蛋白血管緊張素原轉換為血管緊張素，即十肽。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。Mammalian cells are among the best systems for biopharmaceutical production due to their excellence in post - translational modifications. the products they produced are much more similar to their natural forms than those produced by prokaryotic, yeast or insect cells
哺乳動物細胞表達系統具有準確的轉錄后修飾功能，表達的糖基化蛋白藥物在分子結構、理化性質和生物學功能方面最接近天然蛋白分子，是目前重組糖蛋白藥物生產的首選體系。Changes of mitogen - activated protein kinases signaltransduction pathway in ischemia reperfusion injury
再灌注損傷時絲裂原活化蛋白激酶信號轉導通路的變化The gpit - catalyzed reaction represents the final step in the assembly of a gpl - anchored protein, and provides the critical post - translational modification of this class of proteins that allows them to gain entry into er - derived transport vesicles for delivery to the cell surface
Gpit催化的這一反應為gpi化蛋白生物合成的最後一步，也是關鍵的一步。此翻譯后修飾過程保證了這類蛋白質進入出自於er的轉運囊泡，再被傳送至細胞表面。No touches upon multiple aspects of intracellular signaling pathways such as gene activation, protein expression and activity regulation of enzymes
No參與了跨膜信號轉導的多個層面，如基因活化、蛋白質表達和酶活性的調節。The enzyme of microbial transglutaminase ( mtg ) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a - carboxyamide group of glutamine residue in a peptide chain, resulting the cross - linking of proteins
摘要微生物谷氨酰胺轉胺酶（ mtg ）可通過催化蛋白質谷氨酰胺的酰基轉移反應改變蛋白質的結構和性質，是一種有廣泛應用前景的重要新型酶制劑。Many cell signal transduction pathways have been discovered in the regulation of apoptotic induction and reduction. mito - gen - activated protein kinase ( mapk ) is a group of particular ser / thr protein kinases which are activated when its conservative thr and tyr residues are both phosphorylated by upstream mapk kinases
絲裂原活化蛋白激酶（ mapk ）是一群特殊的絲蘇氨酸蛋白激酶，它通過其上游mapkk使其保守的蘇氨酸和酪氨酸殘基雙磷酸化而活化，活化的mapk可轉移到核內，參與基因表達調控。H2o2 acts as signal molecular and intermediate in the downstream of aba or sa signal transduction pathway, which has recently emerged as a secondary messenger of stress signaling
另外，植物細胞內存在許多促分裂原活化蛋白激酶（ mitogen - activatedproteinkinase ， mapk ） ，這些激酶在植物各種脅迫反應的信號轉導過程中起著重要作用。Mapkks ( mitogen - activated protein kinase kinases ) are the key components in the signal transduction passways which regulate a variety of cellular processes in eukaryotes including in plants
促分裂原活化蛋白激酶激酶（ mapkk ）是生物體內信號轉導途徑的關鍵組分之一，已發現它組成的信號轉導級聯途徑在植物中起著多方面的作用。In this paper, the homology of transport proteins of streptomyces lydicus and other actionmycetales bacteria is analysed and their evolutional relation is discussed
將獲得的利迪鏈黴菌的轉運蛋白基因與其他放線菌的轉運蛋白基因進行了同源性分析，探討了基因的進化關系。分享友人