轉化選定 的英文怎麼說
中文拼音 [zhuǎnhuàxuǎndìng]
轉化選定
英文
convert selection-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二，不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一，進入正式試驗期后，在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠，稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液，並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血，姬姆薩-瑞氏染色，計算t淋巴細胞轉化率，計算免疫器官指數，測定肝臟、脾臟銅含量。Intense pulsed light with the specific spectrum penetrate cutis, be transformed to heat
特定譜段的強脈沖光能穿透表皮，被色素團和血紅蛋白有選擇地吸收並轉化為熱能。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Firstly, in spherical coordinate system, the sovp formulation for the time - harmonic electromagnetic fields of the current dipole in conductive infinite - space is derived, using reciprocity theorem and transforming relations between special functions. then, selecting appropriate coordinate system, using superposition principle, the boundary - value problem of modified magnetic vector potential on the problem of a time - harmonic current dipole in spherical conductor is solved and analytical solution is obtained. finally, by means of the addition formulas of legendre polynomial and spherical harmonics function of degree n and order 1, the analytical solution in spherical coordinate system specially located is transformed into that in spherical coordinate system arbitrarily located
首先利用特殊函數間的轉化關系和互易定理推導得到了無限大導體空間中球坐標下時諧電流元電磁場的二階矢量位形式：然後利用疊加原理，選擇合適坐標系，求解了導體球中時諧電流元的修正磁矢量位邊值問題，得到了問題的解析解；最後依據不同坐標系下電磁場解的轉化原理，藉助勒讓德多項式和n次1階球諧函數的加法公式，將坐標系特殊安放時的電磁場解析解變換到坐標系一般安放時的解析解，給出了球內電場和球外磁場的並矢格林函數。In this paper, genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected. the products of indigotin and indirubin in regenerating plantlets had been detected by hplc. the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000
本文利用發根農桿菌rit - dna建立並優化了四倍體菘藍的遺傳轉化體系，並對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測，篩選了優良無性系，也為實現外源抗蟲、抗病等基因的導入奠定了基礎。Cyanopyridine, intermediate of rimifon, was synthesized from 4 - picoline using vanadium oxide as a catalyst in fixed - bed reactor, reached 99 % conversion of 4 - picoline, 88 % selectivity and 87. 12 % yield of 4 - cyanopyridine
以4 -甲基吡啶為原料，在固定床反應器中通過含氧化釩的催化劑發生氣固相接觸氨氧化反應制備雷米封中間體4 -氰基吡啶， 4 -甲基吡啶的轉化率為99 % ， 4 -氰基吡啶的選擇性為88 % ，收率為87 . 12 % 。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。We had observed more than ten selected asteroids using 1 - meter telescope in yunnan observatory during 2000 to 2002. the observational data of seven asteroids had been reduced and analyzed. we have derived their synodic periods and estimated the shape and orientation of rotational axis for one asteroid
為此，我選定了一些直徑在125公里左右的小行星進行觀測和分析；一方面，測定它們的自轉周期，進行「角動量衰減」現象的研究，另一方面，對小行星形狀和自轉軸指向進行估算，為小行星碰撞演化研究提供更為有利的證據。However, genetic improvement by in vitro culture in mustard ( brassica juncea coss. ), especially in chinese mustard, is restricted by the poor frequency of shoot regeneration, and no regeneration system is established yet
芥菜類作物（尤其是中國芥菜）再生率偏低，在遺傳轉化、突變體篩選等方面尚未形成一個高效穩定的再生體系，從而制約了離體條件下遺傳改良的進程。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。Median filtering is chosen to modify the fuzzy edge by using the smoothing arithmetic, sobel operator is used to detect edge because of its filtering characteristic. image is changed from gray - scale to two - valued according to the threshold which is automaticly selected by otsu
選用中值濾波減小平滑演算法導致的邊緣模糊;利用有濾波特性的sobel運算元進行邊緣檢測;使用最大類間方差自動確定閾值，將灰度圖像轉化為二值圖像。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。2. nonlinear source term identification problem about a quasilinear parabolic heat equation is investigated. for the given function determined, the existence and the uniqueness of the solution of the state equation are proved and the dependence of the solution of the state equation on the identification parameter is discussed ; then the identifiability is verified ; through choosing suitable basic functions, the above identification problem can be transformed into a constant coefficients identification problem ; and an practical iterative algorithm for solving the identification problem is presented, the feasibility and validity of the algorithm is verified by the numerical experiments
2 、研究一擬線性拋物型熱傳導方程非線性未知源項的識別問題；對于給定識別函數，論證了狀態方程解的存在惟一性、方程解與識別函數的依賴關系和可識別性；通過選取適當的基函數，把對非線性源項的識別轉化成常系數識別問題；給出了實現非線性源項識別的迭代演算法，通過數值實驗證明了演算法的有效性。With the purpose of better understanding some weak links in this subject, we comprehensively and systematically compared the effects of some typical inhibitors and their different combined ways in inhibiting cd bioavailability in purple soil, further studied the laws and affecting factors of cd ' s transfer and transformation in soil - plant system, revealed the mechanisms inhibitors function, and explored the practicable inhibitor prescriptions and their usage. the results are expected to provide reliable techniques for controlling cd pollution of agricultural soils in chongqing and it is also significant to ameliorate soils polluted by other kinds of heavy metals. comprehensive methods including pot experiment, laboratory culture experiment, kinetic and thermodynamic adsorption - desorption experiment were adopted in this study, and the acidified purple soil was chosen to test, considering that cd is highly active in this soil
本研究針對重慶市農業土壤的污染狀況，選取鎘活性較高的酸化紫色土為供試土壤，採用盆栽試驗、培養試驗、化學熱力學與動力學吸附試驗相結合的方法，系統研究和比較多種調控劑及其不同組合方式對鎘生物活性的效應，並揭示鎘在土壤?植物系統中的遷移轉化規律及影響因素，進一步分析調控劑的作用機理，探尋具有實際應用價值的調控劑配方及合理使用的方法，這不僅可以為重慶市農業土壤鎘污染的防治提供可靠的技術保障，而且對于其它重金屬污染的治理也有一定的理論意義。Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia
挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。Asset appraisal, as a part of neutral activity which tightly accompanied with asset business, put forward a new theme on how to conform to the transformation trend of appraisal research and build up more perfect and integrated appraisal theory under comprehensive, mobile and uncertain conditions which may be helpful to select a proper method in order to promote the quality of appraisal in the activity traditional appraisal theory is built on neo - classical value theory
作為伴隨資產業務、能提供資產現時價值的資產評估中介性活動，如何適應資產評估研究範式的轉化趨勢，在復雜、動態與不確定的環境背景條件下構築完善的資產評估理論，進而選擇適用的資產評估方法以提高資產評估質量是資產評估界面臨的新議題。傳統資產評估理論構建於新古典綜合價值理論基礎之上。The pressure existing between the guide vane and the runner when the pump - turbine of east china tongbai pumped storage power station stops at different loads is analyzed, and the method for determining the best load at which the generator circuit breaker ( gcb ) switches off during operation of the pump - turbine is illuminated
摘要介紹華東桐柏抽水蓄能電站的水泵運行時，不同負荷工況下停機、導葉和轉輪間壓力的變化，闡述了華東桐柏抽水蓄能電站1號機組發電機斷路器開斷的最佳負荷值選定。Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。分享友人