轉基因種子 的英文怎麼說
中文拼音 [zhuǎnjīyīnzhǒngzi]
轉基因種子
英文
genetically modified seed-
This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用，主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。From total 16, 660 dipped inflorescences, l, 268g matured seeds were harvested. in kanamycin - resistent experiment the surface - sterilized non - treated seeds were placed on ms - medium with various kanamycin concentrations
從總共16 ， 660個進行了轉基因操作的花序上共收獲1 ， 268克成熟種子。The tl seeds are screening on the ms medium which contains 50ug / ml kan, and there are about 70 % of the transgenic lines showed the kan - resistance with a ratio of 3 : 1. however, the other lines did n ' t show the ratio consistent with the mendelism. it might prove that most of the transgenic plants only have one insert
對收獲的轉基因番茄t _ 1代種子進行卡那黴素抗性篩選，結果發現， 70以上獨立轉化株系的t _ 1代種子出現了3 1的抗性分離，另一些獨立轉化株系的種子並未表現出符合孟德爾遺傳的分離比，說明大部分er - shsp基因是以單拷貝的方式插入植物基因組。Recent developments in molecular biotechnology are making this possible. in the work described here, three transgenic sublines of embryogenic norway spruce calli : a78 - 3, a78 - 4 and a78 - 5 were studied
隨著分子生物學技術的發展，通過轉基因技術有目的地培育出木質素含量低的挪威雲杉新品種就成為可能。A wheat grain is a genetically modified grass seed, just as a pekinese is a genetically modified wolf
一粒麥子是轉基因的草種，正如獅子狗是轉基因的狼。Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。To meet the increasing demand of this expensive cytokine, we expressed it in transgenic plantso this is a promising way for producing therapeutic proteins in large scale because of its safety, low expense and ease to manipulate
為了滿足對afgf這種價格昂貴的細胞因子的需求，本實驗探索利用轉基因植物表達afgf 。利用轉基因植物大規模生產治療性蛋白是一種非常有潛力的方法，這是因為此種方法安全、生產成本低、易於操作。The pap gene was also transformed into wheat by pollen tube pathway - mediated and 320 grains of transgenic wheat was harvested. the seedlings of the seeds were inoculated with the gav isolate of barley yellow dwarf virus ( bydv ) by schizaphis graminum ( rondani ) and the results revealed that 33 wheat seedlings did not produce the symptom or the symptom was very mild
通過花粉管通道法將pap基因導入小麥，收獲了轉基因小麥種子320粒，將其播種，返青后，直接用麥二叉蚜將大麥黃矮病毒（ bydv ） gav株系接種到幼苗葉片上，觀察發病情況。The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus
本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。The so - called transgenic insects would be resistant to infection by falciparum. through breeding, the altered insects would gradually replace the population of mosquitoes capable of becoming infected with the parasite
這種所謂的轉基因昆蟲對瘧原蟲的感染有抵抗力。通過飼養，這種轉基因昆蟲會逐步取代那些容易被寄生蟲感染的蚊子。To broaden our knowledge about regulatory molecules involved in stress response, we cloned the dreb1c from arabidopsis, and characterized its salt tolerance in transgenic research first. the flowing results were obtained : 1. the dreb1c full length was cloned from arabidopsis genome by pcr, and was inserted into pgem - t - easy vector
為了拓寬對參與植物脅迫應答的調控分子的認識，本論文從dreb1c的克隆著手，以擬南芥為材料，通過轉基因的方法，首次研究了dreb1c這種erebp / ap2類蛋白對植物耐鹽性的的影響，研究結果如下： 1Our results provided direct evidence that pf40 gene play a role in the tissue development, with knockout this gene the lateral buds were not visible. the plant hormones auxin and cytokinin appear to play a major role in controlling this process, therefore, the concertrition of auxin and cytokinin was measured, however, the concentration of the two hormone was variation a little in transgenic plants, but the ratio of the two was more than 5 fold in the pf40 gained function plant than that in wild type. furthermore the pf40 gene effected differentiation of the vascular bundles in different transgenic plants, results showed the pf40 could change both xylary fibers and vessel
進一步的實驗發現pf40基因會影響植物側芽發育的早期，即pf40基因作用在側芽形成與發育的早期。對轉基因穀子的組織切片的分析還表明pf40基因引起不同形式的轉基因穀子的維管組織的發育的差異，轉化正義載體穀子的木質部變多，維管系統增多，而反義或干擾的轉基因穀子的木質部數量變少，木質部分化異常。從轉基因的結果可見pf40基因可以減弱植物的頂端優勢，這種作用是通過改變植物體內激素的含量實現的。He said one example is genetically changed crops that will grow in extreme temperature, dry conditions or poor soil
他說轉基因的農作物就是一個例子，這種作物能在極端的溫度，乾燥的環境或者是貧瘠的土壤下生長。Research field were transgenic breeding, marker assisted breeding, molecular design breeding, related plant functional gene, genetic diversity, molecular maker genetic, basic theory and experiment technology of genetic breeding
研究領域主要涉及轉基因育種、分子標記輔助育種、分子設計育種以及相關的植物功能基因、遺傳多樣性、分子標記遺傳及遺傳育種基礎理論和實驗技術等。Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants
對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。Tobacco seed - determination of genetically modified organism
煙草種子轉基因的測定分享友人