轉基因魚 的英文怎麼說
中文拼音 [zhuǎnjīyīnyú]
轉基因魚
英文
transgenic fish-
Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵,實驗結果如下: ( 1 )顯微注射后,根據胚胎發育分期,胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光,原腸期發熒光的胚胎比例為25 ,後期發育熒光率逐漸下降,肌肉效應期后又相對穩定。Making anti - dis ease breeding researches of fish by transfer gene is a new direction in transfergene researches offish
通過基因轉移進行魚類抗病育種研究,是魚類轉基因研究中的一個新的方向。Elevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus
這樣,可能使得萎縮比目魚肌細胞內鈣離子水平升高,細胞內鈣離子靜息濃度的增加一方面激活calpain ,增加收縮蛋白的降解,使肌肉萎縮;草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶,增加快型mhc基因的表達,使骨骼肌肌球蛋白重鏈( mhc )發生由慢型向快型的轉化。The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus
本研究的目的在於以人的-干擾素基因( ifn - )作為目的基因,與鯉魚-肌動蛋白基因啟動子在體外重組,利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究,其結合了干擾素和基因工程育種抗草魚出血病病毒的優點,以期獲得對草魚出血病具有天然抗性的轉基因魚,並在此基礎上培育出草魚抗病新品系。Highly efficient expression of transgenic genes in early embryo development of zebrafish
病毒序列促轉基因在斑馬魚胚胎發育過程中的高效表達The recombination gene micyoinjected is 5nl dna ( about 106 - 107 ) into everyone oocytes or cytoplasm of grass carp. there were 32197 oocytes of 12 series via micyoinjection and achived 12945 fries in 2000 year, by hatching and raising. genomic dna was extracted from grass carp who were transformated in 1120 grass carp presented postive reaction by blood
實驗通過將hu - - ifn基因重組分子採用顯微注射法將這種重組基因轉化草魚1 - 2細胞期的受精卵,即以narashige顯微注射儀,將5nldna (約10 ~ 6 10 ~ 7分子)直接注射到選擇好的受精卵核的動物極上。Yellow bettas are also called non - red ( nr ) bettas. this mutated gene causes the alteration of red pigment to yellow
黃斗魚又稱為非紅斗魚,基因的突變讓紅色素轉黃。The rapid development of modem molecular biology technique suppies a possible and efficient way for us to solove the problem completely with a comprehensive researches results to transgenic fish in recent years both domestic and aboard, it proves that it " s possible and makes great senses to cultivate a high quality fish to meet the satisifaction of the people
現代分子生物學技術突飛猛進的發展為我們切底解決這一難題提供了可能性和有效途徑。綜觀國內外近些年來對轉基因魚的研究及結果證明,通過轉基因定向培育出符合人們所需要的優良魚種是完全可能的,也是十分有意義的。The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共顯微注射32197粒草魚受精卵,出苗12945尾草魚魚苗,經孵化培育最後獲得1120尾五寸左右草魚,對1120尾草魚提取血液總dna進行pcr和southern雜交檢測, 2000年轉導的草魚的pcr檢測結果,有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶,說明轉化個體整合有huj ifn基因,經southern雜交進一步證實,轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合,但整合位點沒有固定區域,整合的拷貝數存在較大差異。The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23. 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析確證重組質粒在大腸桿菌中能正確轉錄; sds - page分析證明誘導重組質粒表達了融合蛋白,分子量約為50kd ,與預期的26kd的gst帶和23 . 6kd的草魚生長激素基因編碼蛋白質構成的融合蛋白大小一致。The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
應用逆轉錄-聚合酶鏈式反應( rt - pcr )技術克隆得到編碼草魚生長激素( cgh )的基因cdna ,並定向克隆到puc18載體上。And with the seas increasingly depleted by overfishing, much of the world is turning to china ' s land - based fish farms to meet growing demand
隨著海洋資源因過度捕魚而枯竭,為了滿足增長需求,大多數國家和地區正轉向中國的陸基漁場。Glowfish ! the world ' s hottest - selling transgenic treats
熒光魚!世界最熱賣的轉基因禮物The use of retrovirus - mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth
運用反轉錄病毒介雜的插入突變技術,在脊椎動物斑馬魚中已經獲得了許多影響胚胎發育和細胞生長過程的突變體,並找到了對應的基因。分享友人