轉染率 的英文怎麼說

中文拼音 [zhuǎnrǎn]
轉染率 英文
transfection efficiency
  • : 轉構詞成分。
  • : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
  • : 率名詞(比值) rate; ratio; proportion
  1. Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen

    試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏色,計算t淋巴細胞,計算免疫器官指數,測定肝臟、脾臟銅含量。
  2. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響;然後在得到高頻再生體系的基礎上,通過農桿菌介導法化甜菜堿醛脫氫酶( badh )基因,以gus色組織分析為依據探討了影響化效的各種因素,建立了高效、可重復的基因化體系,為四倍體刺槐目的基因的導入打下了基礎。
  3. This paper supplied a new way through variable frequency variable speed ( vfvs ) to change its output flow rate and adding a constant - pressure - drop valve, which could solve the defects in hydraulic pressure servo control system, such as low efficiency in control system, short life, poor ability in resisting pollution, low efficiency in driving system and flow rate, pressure pulse. and this way was proved to be effective

    本論文針對傳統伺服控制系統效不高、元件壽命短、抗污能力差、驅動系統效低、存在流量及壓力脈動等缺陷,利用先進的交流變頻調速技術改變液壓泵速的容積調速新方法,提出了在液壓系統中加一個定差減壓閥的新思路,較好地解決了液壓伺服系統存在的上述問題。
  4. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株( agrobacteriumtumefaciens ) ,含質粒pyhy (目的基因及npt 、 gus基因) ,對白樺試管苗莖段,葉柄,葉片三種外植體進行侵,結果表明:大葉片生長勢強,為基因的最優外植體,能夠達到22 。
  5. Transfection efficiency comparison of cationic liposome - dna complexes and lipid - protamine - dna complexes in vitro

    復合物體外細胞轉染率比較
  6. 3. polyethylenimine ( pei ), a water - soluble cationic polyelectrolyte, has been extensively used as a deformable and mobile substrate for a biomembrane for it high transfection efficiency of chemical - based delivery systems. what ' s more, pei is good buffer in physiological environment ( ph < 9 )

    3 、聚乙烯亞胺( pei )是一種陽離子型的聚合物,由於它在動植物細胞內優良的,而被廣泛的應用,並且在生理環境下( ph 9 )它具有很強的緩沖能力。
  7. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍化法化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的為16 . 7 ,基因槍法的為50 ,因此可能基因槍化法更適于龍牙百合的遺傳化。
  8. Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high

    結果表明: ( 1 )脂質體包裹外源基因精子的方法,可將外源基因導入受精卵中,能夠獲得基因動物,並得到了較高的基因陽性; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。
  9. 3. the transformation rate of pollutants in sediment the main source of organic matters of wetlands is the residua of reed and wild rice

    物在溝渠濕地底泥中的濕地有機質的主要來源是蘆葦和茭草殘株的分解。
  10. The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated. the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one. the cocultivation time is 28 days. the experiment shows that : the erniuxin cotyledon regeneration frequency is very high, and that the dongnong901 hypocotyl regeneration frequency is very high

    確立了農桿菌介導化過程中基因型是影響的主要因素。通過極差分析,基因型為「二牛心」 ,菌液濃度15倍的,外植體為子葉,感方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。
  11. Future strategy and puzzles of heavy ion beam mediated technique in genetic improvement of biological bodies

    重離子輻射提高腺病毒介導小鼠黑色素瘤細胞轉染率的研究
  12. In this review, advances and problems on the study of agrobacterium - mediated genetic transformation of brassica, such as establishment of efficient plant regeneration system and infection method are represented

    從高頻再生體系的建立、農桿菌的侵方法、導入外源基因等方面綜述了蕓薹屬作物的遺傳化研究進展,並對研究中存在的再生成苗、低和篩選方法等問題進行了討論。
  13. The continuing spread of the epidemic and the high rate of infected people in that area of the world have raised the need to establish urgent preventive measures and extend antiretroviral therapy access ( haart )

    在世界上這些地區,這種傳病的持續性傳播和人群的高感迫切要求建立起緊急預防措施和擴展抗逆錄病毒的治療方案( haart ) 。
  14. The average infection rate of mosquitoes was 0. 04 %, and reduced year by year. 3 066 cases were tested by ifa, and with the positives 108, the average positive rate is 3. 52 %

    縱向監測顯示,累計檢出微絲蚴陽性者59例,全部在3 ~ 6年內自然陰,蚊媒自然感為0 . 04 % ,並呈逐年下降趨勢。
  15. Results nanoparticle carrier system can protect the genes, have a high carrying efficiency and a well targeting property, it also can improve the bioavailability, sustained and controlled release

    結果納米顆粒運系統能夠保護被運的基因,有較高的,具有良好的靶向性,並且提高了藥物的生物利用度,顯示出一定的緩控釋作用。
  16. Study on the transfection efficiency of chitosan - dna nanoparticles

    質粒納米粒對腫瘤細胞的研究
  17. An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation

    利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並細胞,以獲得反義rna阻斷vero細胞中相應基因的表達,發現mn 』 ng誘發的非定標性突變頻顯著增高,提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。
  18. The highly effective prevention education that was given to study participants in both arms of the study may have resulted in lower rates of seroconversion than had been anticipated ( two in the tenofovir arm and six in the placebo arm ), decreasing the ability of the study to detect a significant difference in risk of hiv infection

    對兩組參與者進行的極為有效的安全教育也有可能是血清低於預期效果的原因,從而降低了該研究探索泰諾福韋在預防hiv感中和其他療法的差別的能力。
  19. Then we conducted in vitro gene transfer, transfecting p388d1 with recombinant eukaryotic vector and infecting hela with recombinant adenoviruses respectively, in order to observe inhibition of constitutive or inducible cii ta gene expression by introduced complementary antisense rna of c iita and consequent do wnregulation of class ii mhc molecules expression

    結果顯示:含反義c ta的真核表達載體組成型mhc -類分子表達p388d1細胞株,經抗生素抗性篩選后表達mhc -類分子的細胞陽性比對照組下降65
  20. Acetosyringone ( as ) could dramatically improve transformation frequency, its suitable concentration is 20 mg. l " 1. before inoculated with agrobacterium, calli should be precultured 1 ? days, and the appropriate inoculation time is 4 ~ 8 minutes, and then cocultured for 3 ~ 4days. then the cocultured calli were transferred to ms medium containing 400 mg. l " 1 cef

    7為宜;侵前預培養1 2天,可顯著提高化效;侵時間以4 8分鐘為宜;共培養時間3 4天較適合;化后暗培養對化效沒有影響;選擇方法以延遲選擇15天選擇最高。
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