轉染實驗 的英文怎麼說

中文拼音 [zhuǎnrǎnshíyàn]
轉染實驗 英文
transfection experiment
  • 轉 : 轉構詞成分。
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  • 實 : Ⅰ形容詞1 （內部完全填滿 沒有空隙） solid 2 （真實; 實在） true; real; honest Ⅱ名詞1 （實際; 事實...
  • 驗 : 動詞1. （察看; 查考） examine; check; test 2. （產生預期的效果） prove effective; produce the expected result
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. The fuzhou ' s inner river belongs to the rich nutrition type. the water quality changes little comparing with the investigating results ten years ago but the black and foetid rate has been improved. eventually, we analyzed the polluting factor of the inner water and provided some useful suggestions for the management

    實驗結果與十年前內河水質調查的結果進行比較，得出福州內河水質沒有明顯的轉變，但黑臭率有所改善，最後分析了內河污染的成因，並提出治理的意見和建議。
  3. We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker

    在實驗中同時共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
  4. And the differentiation of bmscs were determined to be chondrocyte after g4i8 selection. the bmscs in controlled group were dead about 1 week after g4, 8 selection. conclusions : 1

    對照組bmscs轉染后增殖活性較實驗組低，沒有向軟骨細胞分化的趨勢，經g _ （ 418 ）篩選后1周左右全部死亡。
  5. The change of indoor contaminating material concentration is dynamical simulated by adjusting the flow of fiowrator. at the same time, the path formulas of simulate pollutant source is modified by experiment, and providing theoretical base for dynamic simulation

    通過調節轉子流量計的流量來達到動態模擬室內污染物質濃度的變化情況，並根據對實驗結果的修正得出裝配式潔凈室室內模擬污染源的軌跡方程，為動態模擬提供了理論依據。
  6. P - selectin was negative both in the normal skin and in the postmortem damage group. in the wound specimens aged 1h, we detected expression of icam - 1 in the epidermis, a strong positive staining was observed in 3 hours after wound, and continued to 1 days from the skin was injured after incised, weak staining were showed on epiderm of the injury margin after 3 days incised, and disappeared in 5 days. expression of icam - 1 was also detectable in polymorphonuclear cells ( pmns ) in the wound specim - ents aged 3h

    本實驗研究證明，皮膚損傷后表皮層icam1表達增高，其表達呈現一定時間規律性，在損傷后lh ，損傷邊緣表皮細胞即呈黃褐色染色，傷后3h組， icam一二在表皮層中呈深黃褐色，並一直持續至傷后id ，傷后3d組icam 1染色轉淡，呈黃褐色染色，在傷后sd及7d組， 1 m一二表達基本恢復至正常狀態。
  7. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  8. The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern

    通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。
  9. In vitro cytotoxicities of gcv and ctl to rat mammary cancer cells transfected with hsv - tk b7 gene

    7基因轉染的大鼠乳腺癌細胞殺傷的體外實驗
  10. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  11. Researchers at the conference on retroiruses and opportunistic infections at the los angeles conention center reported that many of the problems could be aoided by judicious use of an experimental drug, an existing aids medication and a widely used cholesterol - lowering agent

    在洛杉磯大會中心召開的關于逆轉錄病毒載體與機會感染會議上，研究者報告說，通過慎重使用一種實驗性藥物可以避免這些問題，此藥為現有的aids附加藥，廣泛用於低膽固醇治療。
  12. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  13. In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively

    本實驗以中國倉鼠卵巢細胞（ cho ）為實驗材料，利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法，在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。
  14. The system is also amenable to genetic transfections, and provides a very short incubation period for studying the role of various genes

    此外，這套實驗系統同時能夠進行基因轉移感染實驗，並可在很短的培育時間下研究各種基因角色。
  15. Upon inoculation of cucumber mosaic virus ( cmv ), the symptom development in the transgenic plants was delayed, one week later than that of the non - transgenic control, and the symptom on the transgenic plants were much milder

    Pcr反應以及southern雜交實驗表明，外源基因片段已經插入到番茄的染色體中。用黃瓜花葉病毒cmv接種轉基因番茄植株，其癥狀出現的時間比非轉基因番茄對照要延遲7天左右。
  16. It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius

    Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。
  17. ( 二 ) using simulative test and onthogonal test to analyze the theories of folded point reactions of chloroamine disinfectant water and the mixed reactions of conveying tubes water by different disinfectant technique. a ) when organic contaminations of original water reach some degree, the reactions of some organic nitrogenous and activated chlorine in original water and the transfoms between organic nitrogenous and ammonia - n will disturb the produce and stability of 1 - chlonoamino. the curve got by the sutra folded point chlorination theory will have greatly changes

    （ 2 ）採用生產性實驗，分析氯胺消毒水的折點反應理論及不同消毒方式的管網水的混合反應，實驗室及生產實驗表明： a ）當原水有機污染達到一定程度，原水中部分有機氮與活性氯的反應以及有機氮與氨氮之間的轉化將干擾一氯胺的生成及穩定，經典折點加氯理論曲線將發生較大變化。
  18. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  19. In this dissertation, the plasmids containing 5s promoter were transfected into hela cells, the transcription sites of rna polymerase iii and its transcripts were detected by fluorescence in situ hybridization ( fish ) to dna, rna and dna - rna, respectively

    本實驗以人的hela細胞為材料，運用電擊轉染、熒光原位雜交並結合激光共聚焦顯微鏡，從dna 、 rna和dna - rna三個水平對rna聚合酶的轉錄位點及其轉錄子的分佈進行研究。
  20. A vaccine is still a long way off, but four clinical trials ? in peru and ecuador, thailand, botswana and also america ? are assessing how well daily anti - retroviral pills, which are normally prescribed to control established hiv infections, prevent the virus infecting healthy people who do dangerous things

    抗愛滋病疫苗還停留在遙遠的未來，但指在控制已感染的病毒擴散和預防高危從業者感染的抗逆轉錄藥物已經投入了臨床實驗? ?在秘魯、厄瓜多、泰國、波札那和美國，研究人員正在評估它的作用。
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