轉移電泳 的英文怎麼說
中文拼音 [zhuǎnyídiànyǒng]
轉移電泳
英文
transfer electrophoresis-
Dab served as chromagen. western blot thirty micrograms of protein extracted from untreated and bfgf, atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane. the blot was probed for nse expression
4westernblot檢測誘導后細胞的nse表達情況從未經處理和經過bfgf ， atra誘導的細胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝膠上電泳並轉移到硝酸纖維素膜上， 4 5脫脂奶粉封閉過夜。6. emsa with coincubation of specific antibodies to the p50 or p65 subunits of nf - b showed a distinct retardation in the mobility with the p65 antibody and a reduction in the intensity of the shifted band with the p50 antibody
6 ，加入抗nf一kbp65和p50的抗體進行電泳遷移率改變實驗，證實nf一kb形成p50 / 65異二聚體參與mbd3基因的轉錄調節。In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot
4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。In this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s. nanchangensis ns3226 from e. coli were tested for developing an conjugation system for s. nanchangensis ns3226. a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e. coli into s. nanchangensis ns3226
將克隆在整合型載體pset152上的變鉛青鏈黴菌1326的dnd基因簇通過接合轉移導入野生型南昌鏈黴菌ns3226中進行異源表達，觀察到接合轉移子的dna獲得了在含fe ~ （ 2 + ）的電泳緩沖液中電泳時降解的表型。Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained
用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定，一是通過電印跡轉移把蛋白質轉到pvdf膜上，再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列，通過網上搜索其同源性對其進行鑒定，並確定該點在凝膠上的位置。分享友人