轉移基因組 的英文怎麼說
中文拼音 [zhuǎnyíjīyīnzǔ]
轉移基因組
英文
transgenome- 轉 : 轉構詞成分。
- 移 : Ⅰ動詞1. (移動) move; remove; shift 2. (改變; 變動) change; alter Ⅱ名詞(姓氏) a surname
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 轉移 : 1 (改換位置) shift; transfer; divert 2 (改變) change; transform 3 [醫學] (擴散) metastasis;...
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Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如:胎球蛋白、組織蛋白酶;和肝臟功能密切相關的如:胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶;與物質能量代謝有關的如: atp合成酶、核糖體蛋白;以及與急相反應有關的如:觸珠蛋白、轉鐵蛋白。They have the unusual property of being able to move around or transpose.
它有一種不尋常的特性,能在基因組內來回移動,也稱為轉位。Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress
最近的研究表明,基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力;結縷草遺傳連鎖圖譜構建取得重要進展;基因克隆和基因資源研究正在展開。The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中,獲得重組轉移載體質粒pbacpak - hbmp 。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段,將其亞克隆于psy538的ecori位點,然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點,再用noti切下同時含有vp3基因和lacz報告基因的片段,再亞克隆于psy681的noti位點,構建出含有vp3基因的重組禽痘病毒轉移載體,為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。5, histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed, which has laid down a foundation for studying chromosome remodeling in vitro
5 ,我們克隆並純化了酵母中的組蛋白乙酞基轉移酶基因gcns和去乙酞化酶基因rpd3 。The recombinant vector was digested with tthllll and the lacz gene from e. coli was inseted in this site, the generated plasmid is designated as pltk - uni
然後定向亞克隆swha基因於多克隆位點,獲得重組轉移載體pltk - ha 。In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針,經[ - ~ ( 32 ) p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達,同時分析了mbd3基因誘導表達的組織特異性,劑效和時效關系;結合mbd3基因啟動子區序列分析,以- 164 ? - 179bp雙鏈dna序列合成探針,經[ - ~ ( 32 ) p ] dctp標記后通過電泳遷移率改變實驗( emsa )和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot
4 .電泳遷移率改變實驗有明顯的滯后帶形成,表明lps刺激后,肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合,參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞,兩輪空斑篩選和純化,獲得重組病毒rbmbacph - egf ,經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因組dnapsti片段為基礎,將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點,再將此插入失活的avec基因片段連接到具有接合轉移功能(含有orit基因)的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區,由此得到重組質粒pc05 。Since 1980s, the risk investment was the focus of the attention of the economic academic community in developed countries especially in u. s. a. not only the basic theory of the indenture economics, information economics and the agency by agreement shows the operational mechanism of the risk investment, but also the means of the experience and empirical analysis are used to study the risk investment ’ s positive affection to the development of the high - tech industry and economy
本文在系統梳理風險投資基本理論與國內外研究進展的基礎上,對美國風險投資的發展歷程及影響因素、風險投資資金來源與風險投資機構的組織形態、風險投資機構的決策過程、風險投資機構與創業企業的契約關系、風險投資的退出途徑等問題展開了具體分析。並且以風險投資對象企業? ?高科技創業企業的治理結構為實例,剖析了以知識經濟為背景的企業權力轉移論,對有關假說進行了經濟學理論與經濟現實相結合的批判。Methods dna chip was used to detect the mrna from 11 human transitional cell carcinoma tissues and to investigate the genes related to signal transduction
方法使用人腫瘤基因表達譜晶元檢測11例膀胱移行細胞癌組織基因表達譜的變化,以尋找與細胞信號轉導相關的差異表達基因。The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用,將它們與nifa基因互換載體后共轉化酵母,仍然可以激活三個報告基因的表達,而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。This paper focuses on the research and measurement of customer satisfaction of hunan power transmission and substation construction company, based on the analysis of their market environment and marketing status quo, and comes out with the conclusion that the construction process of projects, the relationship with the customers, and the service quality are the key factors impacting the satisfaction of power project customers. upon this conclusion, this paper presents new idea to exploit market, and appreciate tactic of marketing mix for electric power construction companies, that is, transferring the focus on those indices for the sake of themselves such as market rate, margin rate etc, to the customer satisfaction index that reflecting customers ’ requirement, and from the phase of ‘ sale ’, to the phase of ‘ do ’ ; at the same time, this paper establishes a set of scientific model and methodology for the marketing decision of power construction companies, instead of the decision making by intuition
本次研究在對湖南送變電公司市場環境和企業營銷現狀進行分析的基礎上,進行湖南送變電公司顧客滿意度調查和測評,得出了工程產品的施工過程、最終輸出以及與顧客關系、服務質量是影響送變電公司顧客滿意度的主要因素的結論,據此改進市場營銷組合策略,為送變電施工企業市場開拓打開了新的思路,將市場營銷策略選擇的著眼點從市場佔有率、利潤率等基於自身需要的指標上轉移到體現顧客需求的顧客滿意度指標上,將關注重心從產品銷售階段轉移到送變電工程產品施工階段;同時也為送變電公司進行營銷決策建立一種科學合理的模型和方法,避免憑自己的直覺作出營銷決策。It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius
Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius
通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中,篩選基因發生重組的菌株。Although it is not entirely clear why the genome is so large and repetitie, researchers theorize that the parasite eoled oer time, preiously inhabiting the intestine and later moing to the urogenital tract, which resulted in increased cell size and, subsequently, a considerably expanded genome
雖然現在還不是十分清楚為什麼基因組如此龐大和高度重復,但研究者們的理論認為:寄生蟲在進化中,早期居住于腸道中,后來才轉移到尿道,導致細胞尺寸增大,后來就相應擴大了基因組。The approach of gene coinjection was firstly used by clark ' s research team in britain, aiming to improve the expression level of foreign gene by its cooperating action. thereafter, it has been increasingly using to find out the impact of gene and its elements on expression. by now, it has been successfully used to produce transgenic mice which contain two or three genes in vivo
外源基因的轉移方法?顯微共注射法,首先由英國的clark為首的研究小組提出,旨在研究外源基因協同作用以提高其表達水平,此後,共注射法用於研究基因和基因構件對表達的影響,並日益受到重視,目前,已成功用於建立轉雙基因小鼠的研究。分享友人