轉肽基酶 的英文怎麼說
中文拼音 [zhuǎntàijī]
轉肽基酶
英文
transpeptidase-
Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如:胎球蛋白、組織蛋白酶;和肝臟功能密切相關的如:胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶;與物質能量代謝有關的如: atp合成酶、核糖體蛋白;以及與急相反應有關的如:觸珠蛋白、轉鐵蛋白。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史,大致經歷了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究階段,產生了一些who / tdr推薦認為很有希望的疫苗候選分子,如谷胱甘肽- s -轉移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖異構酶( tpi ) 、曼氏血吸蟲膜內在蛋白( sm23 )和脂肪酸結合蛋白( fabp , sm14 )等,但其對宿主的保護作用均不甚理想,未能穩定地達到40或以上的保護力水平,因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑,進一步提高其保護力。Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后,通過電擊轉化整合p . pastorisgs115菌株細胞中。Gene inductive expression of bifidobacterium on glutathione s - transferase in ht29 cell line
29細胞谷胱甘肽巰基轉移酶基因的誘導表達For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development
從中隨機選擇一些差異蛋白質點,進行基質輔助激光解吸-電離飛行時間質譜( maldi - tof - ms )測定其膠內酶解后的肽質指紋圖譜,用mascot軟體查詢swiss - port數據庫,初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質,如erp60 、 trypsinogen 、 proelastase 、 lipase等。Cloning and function characterization of glutathione s - transferase gene from tamarix androssowii
紫桿檉柳谷胱甘肽硫轉移酶基因的克隆及功能鑒定Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression
Pcr產物經純化、酶切后,按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶( gst )基因的下游。Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco. the results of fluorescent detection showed gfp localization in the apoplast
Elisa結果顯示,馬鈴薯蛋白酶抑制劑基因的信號肽序列使crylac基因在轉基因煙草中的表達量顯著提高。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶,而目前的研究認為, o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。Human umbilical vein endothelial cells by recombinant human tissue kallikrein gene transfer
重組人組織激肽釋放酶基因轉染人臍靜脈內皮細胞So it has been found that this polymorphism of sod was changed under different experimental conditions, which showed that the polymorphism was not caused by the difference of genes or the difference of length or side groups of the peptide chains which was caused during the processing after transcript or the purification
由此排除了其是由不同基因編碼的同工酶或在轉錄后加工及分離純化過程中形成肽鏈長短不一和酶分子側鏈基團差異等微不均一性所造成的可能性。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術,從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ,克隆抗體重鏈和輕鏈可變區基因,加入連接肽引物( linkerprimer )組裝成單鏈抗體scfv ( singlechainfragmentvariable )基因並用rs引物進行擴增, sfi 、 not酶切,回收后與pcantab5e載體相連,轉化e . colitg1宿主菌,構建單鏈抗體文庫。To allow secretion of the crylac protein into the intercellular space, potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
運用pcr技術克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列,並將其分別連到crylac 、 gfp基因的5 』端,構建植物轉化載體p3301ubisigac和p3301ubisiggfp 。Two expression systems were used, one of which was qiaexpress system, the other glutathione s - transferase ( gst ) gene fusion system
)系統和谷胱甘肽s ?轉移酶( gst )基因融合表達系統。The research of gene expression condition of the glutathione s - transferase in escherichia coli bl21 de
含谷胱甘肽硫轉移酶基因工程菌表達條件研究分享友人