轉酰基酶 的英文怎麼說
中文拼音 [zhuǎnjī]
轉酰基酶
英文
transacylase
-
轉 :
轉構詞成分。
-
酰 :
名詞[化學] (酰基) acyl
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酶 :
名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
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Udp galactose : n acetyl glucosamine galactosyl transferase
乙
酰氨
基葡萄糖半乳糖
基轉移
酶
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Udp gal nac : gm3 n acetyl glucosaminyl transferase
乙
酰氨
基葡萄糖
轉移
酶
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E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以構建的噬菌粒psurfpga
轉化具有琥珀突變的大腸桿菌xl1 - blue ,以輔助噬菌體m13k07超感染,進行青霉素g
酰化
酶基因的表達和在噬菌體表面的展示。
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In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑
轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar
基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙
酰轉移
酶的bar
基因片段,經過適當的修飾構建入真核表達載體。
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Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g
酰化
酶( afpga )
基因構建表達載體pkkfpga和psmlfpga , pkkfpga和psmlfpga均含trc啟動子、青霉素g
酰化
酶基因、 rrnb
轉錄終止子,其中pkkfpga含有氨卞青霉素抗性
基因和cole1高拷貝復制子;而psmlfpga含有四環素抗性
基因和p15a中拷貝復制子。
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The amino group becomes attached to the coenzyme to form pyridoxamine phosphate, and is then transferred to the a - keto acid, which is usually pyruvic acid, oxaloacetic acid, or - ketoglutaric acid
反應中氨
基首先連接在輔
酶上形成磷酸吡哆氨,然後氨
基轉移到-酮酸上,這種-酮酸可以為丙酮酸、草
酰乙酸或是-酮戊二酸。
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Fold recongition and structure simulation for the ricin - like domain of human polypeptide : n - acetylgalactosaminyltransferase
乙
酰氨
基半乳糖
轉移
酶2蓖麻蛋白樣結構域的折疊識別和結構模擬
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Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成,當其易位穿過內質網( er )膜后,被gpi :蛋白質
轉酰胺
基酶( gpit )識別, gpit在移走其羧
基端gpi信號序列的同時將gpi分子連接至新生成的氨
基酸位點上。
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Effect of acupuncture on the expression of choline acetyltransferase mrna in the brain of ovarietomized rats
針刺對去卵巢大鼠腦內膽堿乙
酰轉移
酶基因表達的影響
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Cloning of the glyphosate n - acetyltransferase gene from soil total dna and its characterization of enzyme activity
乙
酰轉移
酶基因的克隆及
酶學特性分析
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An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant
為探索在植物中表達極高g + c含量的pks
基因的可能性,構建了攜帶有編碼fr - 008型pks模塊氨
基端部分的
基因的表達質粒,包括一個酮
基合
酶( ks )和部分
酰基轉移
酶( at )活性結構域。
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Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙
酰氨
基半乳糖
轉移
酶是合成o糖鏈的起始
酶,而目前的研究認為, o -糖
基化與分子及細胞識別、腫瘤的發生發展和
轉移以及胚胎發育等功能密切相關。
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Association between n - acetyltransferase 2 gene polymorphisms and genetic susceptibility to sporadic colorectal adenocarcinoma
乙
酰基轉移
酶2
基因多態性與結腸腺癌易感性的關系
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The enzyme of microbial transglutaminase ( mtg ) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a - carboxyamide group of glutamine residue in a peptide chain, resulting the cross - linking of proteins
摘要微生物谷氨
酰胺
轉胺
酶( mtg )可通過催化蛋白質谷氨
酰胺的
酰基轉移反應改變蛋白質的結構和性質,是一種有廣泛應用前景的重要新型
酶制劑。
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Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法:採用成年大鼠肺組織無血清培養,運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂
酰膽堿( pc )合成量; rt - pcr擴增檢測肺組織中pc合成限速
酶磷酸膽堿二胞苷
酰基轉移
酶( cct ) mrna含量;透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化;免疫組織化學染色檢測glu的受體nmdar1亞單位的表達;生化測定肺組織乳酸脫氫
酶( ldh )釋放量和肺組織勻漿中一氧化氮合
酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化
酶( sod )水平以及丙二醛( mda )含量。
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In bioconversion of a d - amino acid and its derivative, the substrate, 5 ' - monosubstituted hydantoin is firstly transferred into an intermediate, d - carbamyl amino acid by d - hydantoinase, and in turn into an optically active d - amino acid by either d - carbamoylase or chemical, nano2. nowadays, the bioconversion of d - amino acids has been industrialized by enzymes, so called one - step process
D -型氨
基酸及其衍生物的生物
轉化,經歷兩個反應歷程,首先是在海因
酶的作用下,催化5 -單替代海因形成氨甲
酰類氨
基酸中間物,後者再在
酶或化學的作用下,生成相應的光學活性氨
基酸或其衍生物。
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Results n - acetylgalactosaminyltransferases cdna library has been made successfully for the creation of low - density microarrays for gene expression profiling, with which, the different positive signals of n - acetylgalactosaminyltransferases gene expression can be observed in human tumor cells through chemiluminescent detection
通過此晶元採用化學發光法能夠檢測到人腫瘤細胞中的n -乙
酰氨
基半乳糖
轉移
酶基因家族不同程度的陽性信號。
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By sequencing random - selected clones from the library and using bioinformatics, 54 novel expressed sequence tags ( ests ) were obtained, in which there were 14 full - length cdnas, including peroxiredoxin gene, hmg - box gene, ubiquitin gene, signal recognition particle, acetyltransferase gene and lysozyme gene
有14個克隆包含有完整的開放閱讀框,其中包括抗氧化物
酶基因prx 、 dna結合蛋白
基因、泛素
基因、信號識別蛋白
基因、溶菌
酶基因和乙
酰基轉移
酶基因等具有重要生物學功能的
基因。
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As far as the enzymatic activity is concerned, on the one hand, strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids, on the other hand, the higher hydantoinase activity, engineered strain in particular, may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets
Yz - 6菌具有較高的海因酶活性,但n -氨甲酰基d -氨基酸酰氨水解酶活性很低,仍不適合用於d -型氨基酸生產工藝中的一步法轉化。另一方面,含海因酶基因的人工菌株不但酶活性高,也排除了天然菌株轉化生產d -型氨基酸過程中的一些副產物。