轉錄后水平 的英文怎麼說
中文拼音 [zhuǎnlùhòushuǐpíng]
轉錄后水平
英文
post-transcriptional level- 轉 : 轉構詞成分。
- 錄 : Ⅰ名1 (用做記載物的名稱) record; register; collection; selections 2 (姓氏) a surname Ⅱ動詞1 (...
- 水 : 名詞1 (由兩個氫原子和一個氧原子結合而成的液體) water 2 (河流) river 3 (指江、河、湖、海、洋...
- 平 : Ⅰ形容詞1 (沒有高低凹凸 不頃斜) flat; level; even; smooth 2 (高度相同; 不相上下) on the same l...
- 轉錄 : [電學] transcribe; transfer; [聲學] mixing; rerecording; [生物學] transcription; duplicating轉錄...
- 水平 : 1. (跟水面平行的) horizontal;level2. (達到的高度) standard; level
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A full - length enolase cdna of 1734bp from d. salina ( genebank accession no. 245549 ) was acquired by 5 " race and 3 " race. the sequence of enolase gene from d. salina ( dseno gene ) was analyzed by using bioinformadcs
選擇鹽藻23srna作為內標基因,利用半定量rl ,一pcr對經不同滲透震動后( o一48小時)烯醇酶基因轉錄水平的表達量進行了測定。Homology - dependent gene silencing ( hdgs ), which is the generic term for transcriptional gene silencing ( tgs ), post - transcriptional gene silencing ( ptgs ) and rnamediated virus - resistance ( rmvr ) have been shown to frequently occur in transgenic plants
其中,同源依賴的基因沉默又可以分為轉錄水平的基因沉默和轉錄后水平的基因沉默。Expression in vitro cos - 7 cells were transfected with pm, pms, pmi and pmsi constructs by cationic liposom, respectively. 48 hours later, mrna of targets gene were detected by rt - pcr and hil - 12 protein in culture supernatnant and cell lysates were detected by western blotting. p815 cells were transfected with pm and pms constructs and selected by g418
2 .重組質粒在真核細胞中的表達: ( 1 ) pm 、 pms轉染cos一7細胞, 48小時后,用ri 』 - pcr檢測目的基因在mrna水平的表達;轉染p815細胞, g418篩選抗性細胞克隆,用rt - pcr檢測目的基因在mrna水平的表達,結果為陽性,說明在轉錄水平有目的基因的表達。It was indicated that regulation of 20ahsd activity was not due to post - translational modification by either phosphorylation cycle or glycosylation, but rather at the transcription level of 20ahsd gene expression in previous research papers
以往研究表明20 hsd活性的調節不是通過糖基化、磷酸化或去磷酸化等翻譯后修飾實現的,而是以轉錄水平的調節為主。Currently there are many techniques available to screen the gene expression at the transcriptional levels, such as mkna differential display and cdna microarray. although these methods can provide high - throughput information about the differential gene expression, they can
雖然,利用mrna差異顯示技術和基因晶元技術可在轉錄水平高通量的篩選基因表達的差異,但是這些技術都不能提供轉錄后的尤其是蛋白質在翻譯后修飾的信息,而蛋白質通常是細胞內的功能執行者。But the exciting discovery is finding out that resistance training reverses many aspects of this
但經過訓練后, 「老年化的轉錄標簽被明顯逆轉回復到年輕水平,因為大多數基因受年齡和運動雙重影響。These results showed that hsp70 is highly conserved in shrimps. semi - quantitative rt - pcr was developed to study the hsp70 transcription pattern in fenneropenaeus chinensis. the transcription of hsp70 was detected in the major tissues of hepatopancreas, muscles, eyestalks under both normal and heat shocked conditions. the mrna level was increased after the animals were heat shocked in the 3 tissues
為了研究中國對蝦hsp70的轉錄表達,發展了半定量rt - pcr方法,對hsp70轉錄表達分析顯示, hsp70mrna在中國對蝦正常肝胰腺、肌肉、眼柄組織存在組成性表達;在受到整體熱休克后的對蝦肌肉、肝胰腺、眼柄組織hsp70轉錄水平增加。( 6 ) pdgf may initiate neuronal differentiation in nsc. nsc induced by pdgf, transcription and translation level of different thrs genes were up - regulated compared with the nsc induced by serum. the results made a great foundation for the further research of the important role of thrs during the cns development, and have much clinical significance on therapy of cretinism ^ subcretinism a
Thr各亞型在不同分化細胞中表達不一致: ( 0l ;能誘導nsc向少突膠質細胞分化,並通過thr各亞型的時空表達來發揮其重要生理作用門印叩樸能引發vc向神經幾分化,與10血清誘導分化相比能在轉錄與翻譯水平上提高nsc分化后thr各亞型的表達本研究結果將為深入研究t在腦發育中的作用提供寶貴的實驗依據,並對克汀病、亞克汀病以及ms脫髓鞘病等的臨床治療提供新途徑,具有重要指導意義。We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively. at same time the differentiated cell were identified by immunocytochemistry method. results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu
以pdgf 、 t _ 3對nsc進行誘導分化,並分別用rt - pcr半定量法、 elisa法檢測nsc在不同誘導因子作用下分化前後thr各亞型在轉錄與翻譯水平上的表達變化,用免疫細胞化學方法鑒定分化后的細胞類型。However, embryos conceived from brg1 - depleted eggs exhibited a zga phenotype including two - cell arrest and reduced transcription for approximately 30 % of expressed genes
然而,由brg1蛋白缺失的卵細胞受精后形成的胚胎表現出zga表型變化,包括兩細胞期的發育停止以及近30 %表達基因的轉錄水平降低了。Of the remaining 19 patients who underwent withdrawal of antiretroviral therapy ( art ), 17 were rechallenged when liver enzymes had returned to normal or baseline values
7例發展成肝衰竭死亡,其餘的19例中斷了抗逆轉錄病毒治療, 17例患者在轉氨酶恢復正常或基線水平后再次治療。分享友人