轉錄后變化 的英文怎麼說
中文拼音 [zhuǎnlùhòubiànhuà]
轉錄后變化
英文
post-transcriptional change-
Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways
我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified
為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。2. specific mutation from a to c at - 137 bp site upstream from transcription start point had no effect on the inducibility of the promoter in response to sa and bth. 3
在轉錄起始點上游137bp定點將a突變為c后，對化學啟動子的化學誘導應答性沒有明顯的影響，但對誘導效應的向上運輸存在一定程度的阻抑作用。The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively. at same time the differentiated cell were identified by immunocytochemistry method. results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu
以pdgf 、 t _ 3對nsc進行誘導分化，並分別用rt - pcr半定量法、 elisa法檢測nsc在不同誘導因子作用下分化前後thr各亞型在轉錄與翻譯水平上的表達變化，用免疫細胞化學方法鑒定分化后的細胞類型。However, embryos conceived from brg1 - depleted eggs exhibited a zga phenotype including two - cell arrest and reduced transcription for approximately 30 % of expressed genes
然而，由brg1蛋白缺失的卵細胞受精后形成的胚胎表現出zga表型變化，包括兩細胞期的發育停止以及近30 %表達基因的轉錄水平降低了。The labeled cdnas were used to hybridized with the prepared microarray. by using the dna microarray scanner and analysed with the quantarray software package, gene expression profiles of s. cerevisiae in response to heat were studied preliminarily
最後提取熱休克前後酵母細胞的總rna ，經逆轉錄標記合成cdna后與酵母基因表達譜晶元雜交，初步研究了酵母細胞在熱應激時基因表達的變化。分享友人