轉錄基因 的英文怎麼說
中文拼音 [zhuǎnlùjīyīn]
轉錄基因
英文
open gene-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways
我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。It refers to the release of the viral genome from its protective capsid to enable the nucleic acid to be transported within the cell and transcribed to form new progeny virions
它指的是將病毒基因組從它的保護性衣殼中釋放出來，使核酸能在細胞內轉運並能轉錄以形成新的子代病毒。But without rdrp transcription were tested. so it is suggested that tm - 22 is a relative to multi - protein transcriptional coactivator. mip204 and pr - la gene expression can be induced in resistant response and do not relate to rdrp gene transcription
因此，可以認為tm一22基因的編碼蛋白與復合蛋白轉錄因子有關，在抗病反應中能夠誘導五刃滬204和屍尺一ia基因的表達，與rdrp基因的轉錄沒有直接的聯系。For functional genomics, huge est databases from multiple tissues of a number of tree species have been rapidly accumulated, and molecular analyses on secondary growth and wood formation, flowering, and cold hardiness have given some insights into the metabolic pathways of those tree - specific development processes
功能基因組學方面，已分析了主要造林樹種多種組織的轉錄組est序列，對林木次生生長與木材形成、開花和抗寒性的形成等過程開展了功能基因組學研究。Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly
結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。Compared to the prokaryotic gene expressing systems and mammalian gene expressing systems, insect gene expressing systems possess the stronger ability of the transcription and post - translation processing, which also has high production. we can anticipate it will be a kind of potent effective ectogenesis eukaryotic gene expressing system
與原核表達系統和哺乳動物細胞表達系統比較，昆蟲細胞表達系統既有較強轉錄和翻譯后加工修飾能力，又有高表達量等特點，可望成為基因產業中一種比較理想的外源真核基因表達系統。Advances in retrotransposon in animal genome
動物基因組中反轉錄轉座子的研究進展In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。Dreb - type transcription factors can accept stress signals from environment and promote the expression of stress - tolerant genes. four dreb homolog genes, gmdreba, gmdrebb, gmdrebc and osdreb4 - 2 were isolated from soybean [ glycine max ( l. ) merr. ]
Dreb類轉錄因子能接受逆境信號並啟動逆境應答基因的表達。在本研究中，以大豆和水稻為材料，共克隆了4個dreb的同源基因，分別命名為gmdreba ， gmdrebb ， gmdrebc和osdreb4 - 2 。Notch interaction with its ligands induces the cleavage of its intracellular domain ( ic ), and the notch ic translocates to the nucleus and binds to rbp - j, the mammalian homolog of su ( h ), to transactivate transcription of target genes such as e ( spl ) ( enhancer of split ), hesl ( hairy and enhancer of split ) and hes5 four notch receptors and their ligands are differentially and redundantly expressed in a variety of vertebrate tissues
它通過其識別序列（ cogtgggaa ）結合於受調控基因的啟動子區，在轉錄激活因子的驅動下調節細胞分化和個體發育相關基因的表達。在沒有n 。 tch胞內段的情況下， rbpj可與包含sm盯（ silencingmediatorforretlnoldandthyroidhormonereceptor ）和組蛋白去乙酞化酶的轉錄輔助抑制復合物結合，當notch信號被激活時； rbpj可與n 。The two single - pass transmembrane proteins, delta and serrate, have been identified as notch ligands. the transcription factor suppressor of hairless [ su ( h ) ] is the major downstream effector of notch signaling pathway. rbp - j, the mammalian homolog of su ( h ), recognizes the core sequence ( c / tgtgggaa ) of dna
Rbp - j是果蠅促神經發生基因su （ h ） （ suppressorofhairless ）在哺乳動物的同源物，它通過其識別序列（ c tgtgggaa ）結合於受調控基因的啟動子區，在轉錄激活因子的驅動下調節細胞分化和個體發育相關基因的表達。Effect of angiotensin ii on vasopressin gene transcription in the hypothalamus of rats
血管緊張素ii對大鼠下丘腦內血管升壓素基因轉錄的影響Clonal analysis in this study on the eye imaginal disc showed that in the oxt mutant clones, the ci expression was completely deleted
胚胎分析結果表明則t基因的突變會導致wg的轉錄和engrailed基因的表達受抑。This paper sumerised reserach development of plant invertase isoforms, characteristic, function and its gene expression regulatory
綜述了近年來植物轉化酶的種類、特性、功能及其基因表達在轉錄水平和翻譯水平調控機制的研究進展。Roger kornberg won for his work illuminating the process in which genetic information in cells is translated into the proteins that control cellular structure and function
羅傑孔柏格則因其研究說明細胞的基因訊息如何轉錄到控制細胞結構和功能的蛋白質的過程而得獎。Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。They continue to remove molecules until the cell stops transcribing the gene.
他們繼續除去分子，直到細胞不再轉錄基因為止。The transferred gene must also be linked to appropriate regulatory dna sequences to insure that it works in its new environment and is regulated correctly and predictably
另一方面，轉移基因必需被連接到適當的調節基因序列上，才能保證它在新環境下正常轉錄並能進行調節。分享友人